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Sam Minot edited this page Apr 28, 2022 · 5 revisions

How to Make a Genes-in-Genomes Map

The gig-map repository contains a set of tools to help understand what genes are shared across a collection of microbial genomes. This wiki is intended to provide some useful guidance for running the code contained in this software repository. It assumes a basic familiarity with the command line and UNIX shell.

Getting Started

To use this workflow for the first time, you will need to first make sure that a set of basic utilities are set up in your computational environment. The gig-map repository uses Nextflow to coordinate a collection of workflows. The software used at each step is distributed with Docker (or Singularity) images. Finally, the BASH Workbench is the preferred method for configuring the launch of each individual workflow contained within the repository.

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Tutorial

Want to get started analyzing your data? Follow this step-by-step guide to analyze your collection of genes and genomes.

Dive into the Tutorial

Scientific Background

The world of pan-genome analysis is rather complex, and gig-map only tackles a very small part of this intellectual space. We invite you to read more about the scientific background and motivation for the computational tools provided here:

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Tool Inventory

Rather than providing a single stand-alone workflow, gig-map provides a small collection of distinct tools which can be used together in different combinations. These include utilities for downloading genes and genomes, deduplicating gene sequences, performing sequence alignments, and generating interactive visualizations.

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