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# RDI | ||
A Fast Algorithm for Calculating a Novel Degradation Index from RNA-seq Data | ||
## Install RDI | ||
To install RDI, download the source file RDI_0.0.0.9000.tar.gz, then run | ||
``` | ||
install.packages("RDI_0.0.0.9000.tar.gz",repo=NULL,type="source") | ||
``` | ||
|
||
## Run RDI | ||
To run RDI, first create and index the reference transcriptome for calculating RDI | ||
``` | ||
create_index(fasta_file, output_prefix) # fasta file is the reference transcriptome, for example GRCm38 for mouse | ||
``` | ||
Then calculate RDI value for each sample | ||
``` | ||
calculate_RDI(fq_file1, fq_file2, index_path, num_threads, output_dir) | ||
``` |