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added python 3 version, renamed files, tested with Python 3.5.1 :: An…
…aconda 4.0.0 (x86_64)
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#!/usr/bin/env python | ||
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# python 3 | ||
# Author: Stephen Newhouse <[email protected]> | ||
# Date: June 2016 | ||
# see: http://amp.pharm.mssm.edu/Enrichr/help#api for API docs | ||
# | ||
# Usage: python query_enrichr_v0.1.py <genelist> <listdesrciption> <enrichr_library> <enrichr_results> | ||
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import json | ||
import requests | ||
import sys | ||
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print("Enrichr API : Start") | ||
print("Enrichr API : Reading Command Options") | ||
## get command line args | ||
genelist = sys.argv[1] | ||
listdesrciption = sys.argv[2] | ||
enrichr_library = sys.argv[3] | ||
enrichr_results = sys.argv[4] | ||
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# testing | ||
# genelist = "gene_list.txt" | ||
# enrichr_library = "KEGG_2016" | ||
# listdesrciption = "TEST" | ||
# enrichr_results = "example_enrichment_KEGG_2016" | ||
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## Print options | ||
print('Enrichr API : Input file is:', genelist) | ||
print('Enrichr API : Analysis name: ', listdesrciption) | ||
print('Enrichr API : Enrichr Library: ', enrichr_library) | ||
print('Enrichr API : Enrichr Results File: ', enrichr_results) | ||
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# get gene lits | ||
print("Enrichr API : Reading:",genelist) | ||
f = open(genelist) | ||
genes = f.read() | ||
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## enrichr url | ||
ENRICHR_URL = 'http://amp.pharm.mssm.edu/Enrichr/addList' | ||
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# stick gene list here | ||
genes_str = str(genes) | ||
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# name of analysis or list | ||
description = str(listdesrciption) | ||
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# payload | ||
payload = { | ||
'list': (None, genes_str), | ||
'description': (None, description) | ||
} | ||
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# response | ||
print("Enrichr API : requests.post") | ||
response = requests.post(ENRICHR_URL, files=payload) | ||
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if not response.ok: | ||
raise Exception('Error analyzing gene list') | ||
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job_id = json.loads(response.text) | ||
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print('Enrichr API : Job ID:', job_id) | ||
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################################################################################ | ||
# View added gene list | ||
# | ||
ENRICHR_URL_A = 'http://amp.pharm.mssm.edu/Enrichr/view?userListId=%s' | ||
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user_list_id = job_id['userListId'] | ||
#print(user_list_id) | ||
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response_gene_list = requests.get(ENRICHR_URL_A % str(user_list_id)) | ||
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if not response_gene_list.ok: | ||
raise Exception('Error getting gene list') | ||
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print('Enrichr API : View added gene list:', job_id) | ||
added_gene_list = json.loads(response_gene_list.text) | ||
print(added_gene_list) | ||
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################################################################################ | ||
# Get enrichment results | ||
# | ||
ENRICHR_URL = 'http://amp.pharm.mssm.edu/Enrichr/enrich' | ||
query_string = '?userListId=%s&backgroundType=%s' | ||
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## get id data | ||
user_list_id = job_id['userListId'] | ||
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## Libraray | ||
gene_set_library = str(enrichr_library) | ||
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response = requests.get( | ||
ENRICHR_URL + query_string % (str(user_list_id), gene_set_library) | ||
) | ||
if not response.ok: | ||
raise Exception('Error fetching enrichment results') | ||
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print('Enrichr API : Get enrichment results: Job Id:', job_id) | ||
data = json.loads(response.text) | ||
print(data) | ||
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################################################################################ | ||
## Download file of enrichment results | ||
# | ||
ENRICHR_URL = 'http://amp.pharm.mssm.edu/Enrichr/export' | ||
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query_string = '?userListId=%s&filename=%s&backgroundType=%s' | ||
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user_list_id = str(job_id['userListId']) | ||
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filename = enrichr_results | ||
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gene_set_library = str(enrichr_library) | ||
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url = ENRICHR_URL + query_string % (user_list_id, filename, gene_set_library) | ||
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response = requests.get(url, stream=True) | ||
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print('Enrichr API : Downloading file of enrichment results: Job Id:', job_id) | ||
with open(filename + '.txt', 'wb') as f: | ||
for chunk in response.iter_content(chunk_size=1024): | ||
if chunk: | ||
f.write(chunk) | ||
################################################ | ||
print('Enrichr API : Results written to:', enrichr_results + ".txt") | ||
print("Enrichr API : Done") |