tumorevo

Simulate tumor evolution under different spatial constraints. This package aims to be as awesome as demon. tumorevo simulates tumor growth and and produces a Muller plot, a 2D slice of the tumor, and a clone tree.

Installation

$ pip install tumorevo

Usage

tumorevo contains two command line utilities: tumorsim and tumorfig.

Simulating tumor evolution

tumorsim can be used to simulate the evolution of a tumor according to a specified spatial structure.

$ tumorsim --mode 1 --steps 2000 --genes 20 --carrying-capacity 5 --grid-size 20 --division-rate 0.2 --dispersal-rate 0.1
100%|████████████████████| 1999/1999 [00:07<00:00, 251.69it/s]

This will create a folder containing:

• parents.csv: file indicating each clones's parent;
• trace_counts.csv: file indicating the number of cells of each clone at each time step;
• genotypes.csv: file containing the genotypes of each clone;
• grid.csv: file containing the regular grid of genotypes if mode > 0.

Full overview:

$ tumorsim --help
Usage: tumorsim [OPTIONS]

  Simulate tumor evolution under different spatial constraints.

Options:
  -m, --mode INTEGER              Spatial structure.
  -k, --carrying-capacity INTEGER
                                  Deme carrying capacity.
  -g, --genes INTEGER             Number of genes.
  -s, --steps INTEGER             Number of steps in simulation.
  --grid-size INTEGER             Grid size.
  --division-rate FLOAT           Divison rate.
  --mutation-rate FLOAT           Mutation rate.
  --dispersal-rate FLOAT          Dispersal rate.
  -r, --random_seed INTEGER       Random seed for the pseudo random number
                                  generator.
  --log INTEGER                   Logging level. 0 for no logging, 1 for info,
                                  2 for debug.
  -o, --output-path TEXT          Output directory
  --help                          Show this message and exit.

Plotting tumor evolution

tumorfig can be used to create a Muller plot of the tumor's evolution, the 2D spatial organization of the tumor cells, and a clone tree.

$ tumorfig out/trace_counts.csv out/parents.csv --plot --grid-file out/grid.csv --normalize --remove

This will open a figure like this:

Full overview:

$ tumorfig --help
Usage: tumorfig [OPTIONS] GENOTYPE_COUNTS GENOTYPE_PARENTS

  Plot the evolution of a tumor.

Options:
  -c, --cells INTEGER           Number of cells in slice plot.
  -r, --average-radius INTEGER  Average radius of circles in slice plot.
  --grid-file TEXT              Path to grid file.
  --colormap TEXT               Colormap for genotypes.
  --dpi INTEGER                 DPI for figures.
  --plot                        Plot all the figures.
  --do-muller                   Make a Muller plot.
  --do-slice                    Make a slice plot.
  --do-tree                     Make a clone tree plot.
  --normalize                   Normalize the abundances in the Muller plot.
  --labels                      Annotate the clone tree plot.
  --remove                      Remove empty clones in the clone tree plot.
  -o, --output-path TEXT        Directory to write figures into.
  --help                        Show this message and exit.