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Update README.md, CHANGELOG.md, duplex_tools/__init__.py
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![Oxford Nanopore Technologies logo](https://github.com/nanoporetech/medaka/raw/master/images/ONT_logo_590x106.png) | ||
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> **Deprecation notice** | ||
> Please note that the functionality in this repository is superseded by integrated pairing in [Dorado](https://github.com/nanoporetech/dorado/tree/master/dorado). | ||
> This means it's no longer necessary to use pairing or dorado_stereo.sh in order to perform end-to-end duplex calling. | ||
# Duplex Tools | ||
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Duplex Tools contains a set of utilities for dealing with Duplex sequencing | ||
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This will create an (unmapped) .sam file which has a mapping between the signal and bases. | ||
`--emit-moves` allows for additional pairs to be found in step 2b. | ||
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$ dorado basecaller [email protected] pod5s/ --emit-moves > unmapped_reads_with_moves.sam | ||
$ dorado basecaller [email protected] pod5s/ --emit-moves > unmapped_reads_with_moves.bam | ||
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### 2a) Find duplex pairs for Dorado stereo/basespace basecalling | ||
This will detect the majority of pairs and put them in the `pairs_from_bam` directory. | ||
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