Panel Tracing
The panel under the 
The set of toggle buttons defines, what type of ROI will be added to the ROI manager upon point addition.
For the description of ROI interface please refer to Creating ROIs page.
A general ROI render settings and semi-auto tracing parameters are configured in settings 
This subpanel contains an interactive list of ROIs. A selected ROI is usually rendered with different colors (they can be defined in settings).
A name for a new ROI is generated automatically and can be changed using Rename button.
If a ROI is a member of Groups, its name will contain a prefix in square brackets (first three letters of the group name).
Delete and Deselect buttons will do exactly what one would expect them to do.
This dialog under the
The first two parameters specify colors of active (selected) ROI.
# sectors line render parameter defines how many sectors are created for the visualization of pipe (cylinder) around PolyLine3D and LineTrace3D ROIs. It affects only visualization, the intensity measurements will always happen around a circle (up to pixelization rounding). Here is an example of the same ROI render with sectors number 4, 10 and 20:
ROI Shape interpolation affects both appearance and measurements of ROIs. In Voxel mode shapes are considered as a groups of voxels, while in Subvoxel they are smoothened with Trace smoothing window parameter below or directly interpolated. Both parameters are synchronized with corresponding Measure settings values. For more details on ROI shapes, see ROI shape interpolation page.
Curve thickness along axes parameters specify the approximate thickness of curves of interest in each dimension (in pixels!). A good estimate for these values can be made by plotting intensity profile perpendicular to a curve in each dimension and fitting it with Gaussian function. SD of this Gaussian is the value that needed.
Basically it is a SD of Gaussian Derivative convolution filters for each dimension used to calculate Hessian matrix.
Trace box size (px) specifies dimension of TraceBox. Basically, it is length of the edge of the cube/box.
Trace box screen fraction (0-1): How much plugin should zoom in to the TraceBox. In case of 1 TraceBox will occupy the whole viewer window.
Trace box advance [0-center..1-edge] initial TraceBox always has the initial point in the middle of the box. But after advancing it (with T) shortcut, this option specifies, where TraceBox will be positioned with respect to the last added point. The plugin calculates the last direction of the curve and positions the last point along this direction either at the edge (value 1) or center of the box (value 0).
Trace only cropped volume checkbox decides, in case of crop applied to a dataset, whether the tracing will continue outside the cropped volume.