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InSilico SCReening for SC2 guides

Please refer to wiki for details of the pipeline.

Create the conda environment isscr

conda env update --name isscr -f env.yml

Compuate exact match via grep

paste - - < guides.fa  | awk '{print $2, substr($1,2)}' | xargs -Ixx -P 8 bash -c "bash grep_kmer_counts.sh xx"

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InSilico off-target screening for SC2 guides.

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