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add links to relevant sections of materials
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tavareshugo committed Jan 25, 2024
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17 changes: 14 additions & 3 deletions materials/03-case_studies/03-eqa.md
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Expand Up @@ -201,8 +201,9 @@ To make sure no date information is lost due to _Excel_'s behaviour, it's a good
At this point we are ready to start our analysis with the first step: generating a consensus genome for our samples.
We will use a standardised pipeline called _viralrecon_, which automates most of this process for us, helping us be more efficient and reproducible in our analysis.

:::{.callout-note}
If you need to revise how the `nf-core/viralrecon` pipeline works, please consult the [Consensus Assembly](../02-isolates/01-consensus.md) section of the materials.

:::

### Samplesheet

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## Consensus Quality

Once your workflow is complete, it's time to assess the quality of the initial assembly.
Once your workflow is complete, it's time to assess the quality of the assembly.

:::{.callout-note}
If you need to revise how to interpret the quality control report, please consult the [Quality Control](../02-isolates/02-qc.md) section of the materials.
:::

### Coverage

At this stage we want to identify issues such as:

- Any samples which have critically low coverage. There is no defined threshold, but samples with less than 85% coverage should be considered carefully.
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Make a note of any samples that you think are problematic.
You can discuss with your colleagues and compare your results/conclusions to see if you reach similar conclusions.

:::


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- **Clustering:** assess how many clusters of sequences we have, based on a phylogenetic analysis.
- **Integration & Visualisation:** cross-reference different results tables and produce visualisations of how variants changed over time.

:::{.callout-note}
If you need to revise these topics, please consult the [Lineage Assignment](../02-isolates/03-lineages.md) and [Phylogenetics](../02-isolates/04-phylogeny.md) sections of the materials.
:::

### Lineage Assignment

Although the _Viralrecon_ pipeline can run _Pangolin_ and _Nextclade_, it does not use the latest version of these programs (because lineages evolve so fast, the nomenclature constantly changes).
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