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version 2.1, changelog, manual
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andrewprzh committed Jan 18, 2022
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2 changes: 1 addition & 1 deletion LICENSE
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IsoQuant: A tool for isoform assignment and quantification fror long and barcoded reads
Copyright (c) 2020-2021 Saint Petersburg State University
Copyright (c) 2020-2022 Saint Petersburg State University

IsoQuant is free software; you can redistribute it and/or modify
it under the terms of the GNU General Public License, Version 2,
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10 changes: 2 additions & 8 deletions README.md
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[![GitHub Downloads](https://img.shields.io/github/downloads/ablab/IsoQuant/total.svg?style=social&logo=github&label=Download)](https://github.com/ablab/IsoQuant/releases)


# IsoQuant 2.0 manual
# IsoQuant 2.1 manual

1. [About IsoQuant](#sec1) </br>
    1.1. [Supported data types](#sec1.1)</br>
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IsoQuant is a tool for reference-based analysis of long RNA reads, such as PacBio or Oxford Nanopores. IsoQuant maps reads to the reference genome and assigns them to the annotated isoforms based on their intron and exon structure. IsoQuant is also capable of discovering various modifications, such as intron retention, alternative splice sites, skipped exons etc. IsoQuant further performs gene, isoform, exon and intron quantification. If reads are grouped (e.g. according to cell type), counts are reported according to the provided grouping. In addition, IsoQuant generates discovered transcript models, including novel ones.

IsoQuant version 2.0.0 was released under GPLv2 on October 7th, 2021 and can be downloaded from [https://github.com/ablab/IsoQuant](https://github.com/ablab/IsoQuant).
IsoQuant version 2.1.0 was released under GPLv2 on January 18th, 2022 and can be downloaded from [https://github.com/ablab/IsoQuant](https://github.com/ablab/IsoQuant).

#### IsoQuant pipeline
![Pipeline](figs/isoquant_pipeline.png)
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`--delta`
Delta for inexact splice junction comparison, chosen automatically based on data type.

`--read_assignments`
Prefix of intermediate read assignments located in `<output_dir>/<sample_dir>/aux/`.
These files are kept only when `--keep_tmp` is set.

`--keep_tmp` Do not remove temporary files.


### Examples
<a name="examples"></a>
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2 changes: 1 addition & 1 deletion VERSION
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2.0.0
2.1.0
8 changes: 8 additions & 0 deletions changelog.html
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<title>IsoQuant changelog</title>
<body>

<h3>IsoQuant 2.1.0, 19 Jan 2022</h3>

<p>Improved recall by introducing relative coverage cutoffs.</p>

<p>More careful handling of transcript terminal positions.</p>

<p>Fixed GTF to BED conversion.</p>

<h3>IsoQuant 2.0.0, 7 Oct 2021</h3>

<p>Completely new transcript discovery algorithm with significantly higher recall.</p>
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