diff --git a/samples/drivers/led_ws2812/README.rst b/samples/drivers/led_ws2812/README.rst
index 5529604b0a8cae..183875520f2d27 100644
--- a/samples/drivers/led_ws2812/README.rst
+++ b/samples/drivers/led_ws2812/README.rst
@@ -102,6 +102,8 @@ This sample uses different drivers depending on the selected board:
 I2S driver:
 
 - thingy52_nrf52832
+- nrf5340dk_nrf5340 (3.3V logic level, a logic level shifter may be required)
+    - should work for other boards featuring an nRF5340 host processor
 
 SPI driver:
 
diff --git a/samples/drivers/led_ws2812/boards/nrf5340dk_nrf5340.conf b/samples/drivers/led_ws2812/boards/nrf5340dk_nrf5340.conf
new file mode 100644
index 00000000000000..f5d64aaf5da02e
--- /dev/null
+++ b/samples/drivers/led_ws2812/boards/nrf5340dk_nrf5340.conf
@@ -0,0 +1,5 @@
+CONFIG_SPI=n
+
+CONFIG_I2S=y
+CONFIG_WS2812_STRIP=y
+CONFIG_WS2812_STRIP_I2S=y
diff --git a/samples/drivers/led_ws2812/boards/nrf5340dk_nrf5340.overlay b/samples/drivers/led_ws2812/boards/nrf5340dk_nrf5340.overlay
new file mode 100644
index 00000000000000..738e79115326fe
--- /dev/null
+++ b/samples/drivers/led_ws2812/boards/nrf5340dk_nrf5340.overlay
@@ -0,0 +1,36 @@
+#include <zephyr/dt-bindings/led/led.h>
+
+&pinctrl {
+	i2s0_default_alt: i2s0_default_alt {
+		group1 {
+			/* Default I2S config for the nRF5340, P1.13 is the output */
+			psels = <NRF_PSEL(I2S_SCK_M, 1, 15)>,
+				<NRF_PSEL(I2S_LRCK_M, 1, 12)>,
+				<NRF_PSEL(I2S_SDOUT, 1, 13)>,
+				<NRF_PSEL(I2S_SDIN, 1, 14)>;
+		};
+	};
+};
+
+i2s_led: &i2s0 {
+	status = "okay";
+	pinctrl-0 = <&i2s0_default_alt>;
+	pinctrl-names = "default";
+};
+
+/ {
+	led_strip: ws2812 {
+		compatible = "worldsemi,ws2812-i2s";
+
+		i2s-dev = <&i2s_led>;
+		chain-length = <42>; /* arbitrary; change at will */
+		color-mapping = <LED_COLOR_ID_GREEN
+					LED_COLOR_ID_RED
+					LED_COLOR_ID_BLUE>;
+		reset-delay = <500>;
+	};
+
+	aliases {
+		led-strip = &led_strip;
+	};
+};