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CDS ID tag incompatibility - 'ID=CDS:ENSB:O-Ndl8aNBRf4G1w' needs to be 'ID=ENSB_O-Ndl8aNBRf4G1w' #623

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NickJD opened this issue Aug 9, 2024 · 0 comments

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@NickJD
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NickJD commented Aug 9, 2024

Hi,
Just posting this if anyone else has a similar problem.
As the title shows, to get Roary to work correctly (It ran as if all was fine but in the final step it reported only 1 gene as present), we need to modify the CDS lines in the GFF file as below:
'ID=CDS:ENSB:O-Ndl8aNBRf4G1w' needs to be 'ID=ENSB_O-Ndl8aNBRf4G1w'
I guess this is because the first ':' is treated as a separator and thus 'all' genes are named 'CDS', hence only one gene is reported in the end.

Hope this helps someone.

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