diff --git a/bindings/pyroot/pythonizations/CMakeLists.txt b/bindings/pyroot/pythonizations/CMakeLists.txt
index 6521c568b496f..eecd7efd3d058 100644
--- a/bindings/pyroot/pythonizations/CMakeLists.txt
+++ b/bindings/pyroot/pythonizations/CMakeLists.txt
@@ -114,7 +114,6 @@ set(py_sources
 
 set(cpp_sources
     src/PyROOTModule.cxx
-    src/PyROOTWrapper.cxx
     src/RPyROOTApplication.cxx
     src/GenericPyz.cxx
     src/TClassPyz.cxx
diff --git a/bindings/pyroot/pythonizations/src/PyROOTModule.cxx b/bindings/pyroot/pythonizations/src/PyROOTModule.cxx
index 7ccd1d6d86d69..13873dfe2623f 100644
--- a/bindings/pyroot/pythonizations/src/PyROOTModule.cxx
+++ b/bindings/pyroot/pythonizations/src/PyROOTModule.cxx
@@ -11,8 +11,8 @@
 
 // Bindings
 #include "PyROOTPythonize.h"
-#include "PyROOTWrapper.h"
 #include "RPyROOTApplication.h"
+#include "TMemoryRegulator.h"
 
 // Cppyy
 #include "CPyCppyy/API.h"
@@ -34,7 +34,7 @@
 
 namespace PyROOT {
 
-PyObject *gRootModule = nullptr;
+R__EXTERN PyObject *gRootModule = nullptr;
 
 PyObject *RegisterConverterAlias(PyObject * /*self*/, PyObject *args)
 {
@@ -62,6 +62,20 @@ PyObject *RegisterExecutorAlias(PyObject * /*self*/, PyObject *args)
 
 } // namespace PyROOT
 
+namespace {
+
+PyROOT::RegulatorCleanup &GetRegulatorCleanup()
+{
+   // The object is thread-local because it can happen that we call into
+   // C++ code (from the PyROOT CPython extension, from CPyCppyy or from cling)
+   // from different Python threads. A notable example is within a distributed
+   // RDataFrame application running on Dask.
+   thread_local PyROOT::RegulatorCleanup m;
+   return m;
+}
+
+} // namespace
+
 // Methods offered by the interface
 static PyMethodDef gPyROOTMethods[] = {
    {(char *)"AddCPPInstancePickling", (PyCFunction)PyROOT::AddCPPInstancePickling, METH_VARARGS,
@@ -144,8 +158,13 @@ extern "C" PyObject *PyInit_libROOTPythonizations()
    // keep gRootModule, but do not increase its reference count even as it is borrowed,
    // or a self-referencing cycle would be created
 
-   // setup PyROOT
-   PyROOT::Init();
+   // Initialize and acquire the GIL to allow for threading in ROOT
+#if PY_VERSION_HEX < 0x03090000
+   PyEval_InitThreads();
+#endif
+
+   // Memory management
+   gROOT->GetListOfCleanups()->Add(&GetRegulatorCleanup());
 
    // signal policy: don't abort interpreter in interactive mode
    CallContext::SetGlobalSignalPolicy(!gROOT->IsBatch());
diff --git a/bindings/pyroot/pythonizations/src/PyROOTWrapper.cxx b/bindings/pyroot/pythonizations/src/PyROOTWrapper.cxx
deleted file mode 100644
index 26cd81d9d9ee3..0000000000000
--- a/bindings/pyroot/pythonizations/src/PyROOTWrapper.cxx
+++ /dev/null
@@ -1,42 +0,0 @@
-// Author: Enric Tejedor CERN  06/2018
-// Original PyROOT code by Wim Lavrijsen, LBL
-
-/*************************************************************************
- * Copyright (C) 1995-2018, Rene Brun and Fons Rademakers.               *
- * All rights reserved.                                                  *
- *                                                                       *
- * For the licensing terms see $ROOTSYS/LICENSE.                         *
- * For the list of contributors see $ROOTSYS/README/CREDITS.             *
- *************************************************************************/
-
-// Bindings
-#include "PyROOTWrapper.h"
-#include "TMemoryRegulator.h"
-
-// ROOT
-#include "TROOT.h"
-
-namespace PyROOT {
-R__EXTERN PyObject *gRootModule;
-}
-
-PyROOT::RegulatorCleanup &GetRegulatorCleanup()
-{
-   // The object is thread-local because it can happen that we call into
-   // C++ code (from the PyROOT CPython extension, from CPyCppyy or from cling)
-   // from different Python threads. A notable example is within a distributed
-   // RDataFrame application running on Dask.
-   thread_local PyROOT::RegulatorCleanup m;
-   return m;
-}
-
-void PyROOT::Init()
-{
-   // Initialize and acquire the GIL to allow for threading in ROOT
-#if PY_VERSION_HEX < 0x03090000
-   PyEval_InitThreads();
-#endif
-
-   // Memory management
-   gROOT->GetListOfCleanups()->Add(&GetRegulatorCleanup());
-}
diff --git a/bindings/pyroot/pythonizations/src/PyROOTWrapper.h b/bindings/pyroot/pythonizations/src/PyROOTWrapper.h
deleted file mode 100644
index 2847bf6f404ad..0000000000000
--- a/bindings/pyroot/pythonizations/src/PyROOTWrapper.h
+++ /dev/null
@@ -1,24 +0,0 @@
-// Author: Enric Tejedor CERN  06/2018
-// Original PyROOT code by Wim Lavrijsen, LBL
-
-/*************************************************************************
- * Copyright (C) 1995-2018, Rene Brun and Fons Rademakers.               *
- * All rights reserved.                                                  *
- *                                                                       *
- * For the licensing terms see $ROOTSYS/LICENSE.                         *
- * For the list of contributors see $ROOTSYS/README/CREDITS.             *
- *************************************************************************/
-
-#ifndef PYROOT_ROOTWRAPPER_H
-#define PYROOT_ROOTWRAPPER_H
-
-#include "Python.h"
-
-namespace PyROOT {
-
-// initialize ROOT
-void Init();
-
-} // namespace PyROOT
-
-#endif // !PYROOT_ROOTWRAPPER_H