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Same statistical results from different snpeff runnings. #573

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Khewahah opened this issue Dec 1, 2024 · 0 comments
Open

Same statistical results from different snpeff runnings. #573

Khewahah opened this issue Dec 1, 2024 · 0 comments

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@Khewahah
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Khewahah commented Dec 1, 2024

Describe the issue
I got very similar even same results(.html) after running snpeff with several population vcf files which are seperated from a total vcf file.

To Reproduce

  1. SnpEff version: V5.1
  2. Genome version: assembled by ourselves
  3. SnpEff full command line:
    java -jar snpEff.jar build -gff3 -v genome.ver.2.0.0 -config ~/softwares/snpEff-5.1/snpEff.config
    java -Xmx8g -Dsnpeff.parallel.threads=64 -jar snpEff.jar -v -canon genome.ver.2.0.0 -stats temp1.indel.html temp1.indel.vcf.gz | bgzip > anno_temp1.indel.vcf.gz
  4. Output:
    image
    image
    image
    i'm not sure whether you can see these pics, but they(html files) are totally same.
    And this is the seperation command:
    bcftools view --threads 64 -S sample.list --force-samples merged.indel.vcf.gz | bgzip > temp1.snp.vcf.gz

Expected behavior
So, I need you tell me what to do to get real annotation of different samples.

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