ERROR:ont_fast5_api.conversion_tools.multi_to_single_fast5:Could not convert Multi->Single for file type 'single-read' with path

[akk01]

I am getting this error when I run multi_to_single_fast5 with Fast5:

ERROR:ont_fast5_api.conversion_tools.multi_to_single_fast5:Could not convert Multi->Single for file type 'single-read' with path

How to check if the files are multi-fast5? What about the case with mixture of both single- and multi-read fast5.

[fbrennen]

Hi @akk01 -- we don't currently have a good solution for when you end up with a folder of mixed single- and multi-read fast5 files, but we can put one together. You've definitely got both types in your folder?

[akk01]

I am not sure if the folder contains both single and multi-read fast5.

[akk01]

I came across a possible solution of the above problem:
https://bioinformatics.stackexchange.com/questions/7034/questions-regarding-nanopore-sequencing-analysis

[fbrennen]

A good answer there -- we'll update our scripts to work on both file types anyways.