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# Install CAPS, either as dynamic or static binary. Then, install the # rest of the programs that AutoCoEv drives. Below are instructions for # Debian/Ubuntu, tested on Ubuntu 20.04 (LTS). For the programs that are # not available as packages, you may wish to check the corresponding web- # sites to get the latest versions. Patches are discussed in the bottom. # These are available as packages: sudo apt install muscle mafft datamash ncbi-blast+ exonerate prank squizz parallel phyml r-base # Build Guidance (http://guidance.tau.ac.il/) it needs BioPerl and co: sudo apt install bioperl sudo apt install libbio-coordinate-perl libbio-db-ace-perl libbio-eutilities-perl libbio-procedural-perl libbio-samtools-perl wget http://guidance.tau.ac.il/guidance.v2.02.tar.gz tar xvf guidance.v2.02.tar.gz cd guidance.v2.02 make cd .. # I know /usr/local/lib is better, but for now place it here: sudo cp -a guidance.v2.02 /usr/lib/guidance # Get Gblocks (http://molevol.cmima.csic.es/castresana/Gblocks.html): wget http://molevol.cmima.csic.es/castresana/Gblocks/Gblocks_Linux64_0.91b.tar.Z tar xvf Gblocks_Linux64_0.91b.tar.Z sudo cp Gblocks_0.91b/Gblocks /usr/local/bin # Get SeqKit (https://bioinf.shenwei.me/seqkit/) wget https://github.com/shenwei356/seqkit/releases/download/v2.1.0/seqkit_linux_amd64.tar.gz tar xvf seqkit_linux_amd64.tar.gz sudo cp seqkit /usr/local/bin # Build TreeBeST Ensembl mod (https://github.com/Ensembl/treebest/) sudo apt install flex bison wget https://github.com/Ensembl/treebest/archive/347fa82/treebest-347fa82a0ce1c169849053fdc9ff7d19d221f290.tar.gz tar xvf treebest-347fa82a0ce1c169849053fdc9ff7d19d221f290.tar.gz cd treebest-347fa82a0ce1c169849053fdc9ff7d19d221f290 make sudo cp treebest /usr/local/bin # AutoCoEv uses phyml executable directly, not through the wrapper # provided by Debian/Ubuntu. Therefore, make a symlink: cd /usr/local/bin ln -s ../../lib/phyml/bin/phyml . # AutoCoEv expects to find MAFFT aliases in your $PATH. Either add # /usr/lib/mafft/bin to your $PATH or create symlinks, like this: cd /usr/local/bin ln -s ../../lib/mafft/mafft-linsi . # It has come to my attention, that fastaindex produces an empty file on # Debian and Ubuntu (at least in a virtual machine). I am trying to # resolve the issue at the moment. If you have this problem, download # the indexed database 'odb10v1_all_fasta.tab.index.tar.gz' from: # https://seafile.utu.fi/d/a8de85062abf4ab68de9/ # Then extract and place in its proper location: tar xvf odb10v1_all_fasta.tab.index.tar.gz mv odb10v1_all_fasta.tab.index /var/tmp/DB10v1 # Check caps-dynamic and caps-statit for building and installing CAPS2. # You can just grab the precompiled static binary and go with it.