Spot size

[AlbertoFabbri93]

I am using CosMx for my analysis and it is not entirely clear to me what is the spot size. The documentation states:

tol: the tolerance factor to increase the robustness when comparing the center-to-center distance against the interaction.range. This can be the half value of cell/spot size in the unit of um. If the cell/spot size is not known, we provide a function computeCellDistance to compute the cell center-to-center distance. tol can be the the half value of the minimum center-to-center distance. Of note, CellChat does not need an accurate tolerance factor, which is used for determining whether considering the cell-pair as spatially proximal if their distance is greater than interaction.range but smaller than “interaction.range + tol”.

What is the spot size exactly? CosMx, in the metadata, provides the width and height of every cell, can these be used to decide the tolerance value? E.g. tol = min(height, width)/2