diff --git a/mocks/app/routes/directory/_error.tsx b/mocks/app/routes/directory/_error.tsx
new file mode 100644
index 0000000..11fd6fd
--- /dev/null
+++ b/mocks/app/routes/directory/_error.tsx
@@ -0,0 +1,7 @@
+import type { ErrorHandler } from 'hono'
+
+const handler: ErrorHandler = (error, c) => {
+  return c.render(<h1>Custom Error in /directory: {error.message}</h1>)
+}
+
+export default handler
diff --git a/mocks/app/routes/directory/sub/throw_error.tsx b/mocks/app/routes/directory/sub/throw_error.tsx
new file mode 100644
index 0000000..dc125fb
--- /dev/null
+++ b/mocks/app/routes/directory/sub/throw_error.tsx
@@ -0,0 +1,5 @@
+import { createRoute } from '../../../../../src/factory'
+
+export default createRoute(() => {
+  throw new Error('Foo')
+})
diff --git a/mocks/app/routes/directory/throw_error.tsx b/mocks/app/routes/directory/throw_error.tsx
new file mode 100644
index 0000000..bdf2fb1
--- /dev/null
+++ b/mocks/app/routes/directory/throw_error.tsx
@@ -0,0 +1,5 @@
+import { createRoute } from '../../../../src/factory'
+
+export default createRoute(() => {
+  throw new Error('Foo')
+})
diff --git a/src/server/server.ts b/src/server/server.ts
index ceefcba..7121836 100644
--- a/src/server/server.ts
+++ b/src/server/server.ts
@@ -111,6 +111,8 @@ export const createApp = <E extends Env>(options: BaseServerOptions<E>): Hono<E>
     return paths
   }
 
+  const errorHandlerMap: Record<string, ErrorHandler> = {}
+
   for (const map of routesMap) {
     for (const [dir, content] of Object.entries(map)) {
       const subApp = new Hono<{
@@ -186,8 +188,19 @@ export const createApp = <E extends Env>(options: BaseServerOptions<E>): Hono<E>
         }
       }
 
-      // Error
-      applyError(subApp, dir, errorMap)
+      // Get an error handler
+      const errorHandler = getErrorHandler(dir, errorMap)
+      if (errorHandler) {
+        errorHandlerMap[dir] = errorHandler
+      }
+
+      // Apply an error handler
+      for (const [path, errorHandler] of Object.entries(errorHandlerMap)) {
+        const regExp = new RegExp(`^${path}`)
+        if (regExp.test(dir) && errorHandler) {
+          subApp.onError(errorHandler)
+        }
+      }
 
       let rootPath = getRootPath(dir)
       if (trailingSlash) {
@@ -241,24 +254,23 @@ function applyNotFound(
   }
 }
 
-function applyError(
-  app: Hono<{ Variables: Variables }>,
-  dir: string,
-  map: Record<string, Record<string, ErrorFile>>
-) {
+function getErrorHandler(dir: string, map: Record<string, Record<string, ErrorFile>>) {
   for (const [mapDir, content] of Object.entries(map)) {
     if (dir === mapDir) {
       const errorFile = content[ERROR_FILENAME]
       if (errorFile) {
-        const errorHandler = errorFile.default
-        app.onError(async (error, c) => {
-          const importingIslands = errorFile[IMPORTING_ISLANDS_ID]
-          if (importingIslands) {
-            c.set(IMPORTING_ISLANDS_ID, importingIslands)
+        const matchedErrorHandler = errorFile.default
+        if (matchedErrorHandler) {
+          const errorHandler: ErrorHandler = async (error, c) => {
+            const importingIslands = errorFile[IMPORTING_ISLANDS_ID]
+            if (importingIslands) {
+              c.set(IMPORTING_ISLANDS_ID, importingIslands)
+            }
+            c.status(500)
+            return matchedErrorHandler(error, c)
           }
-          c.status(500)
-          return errorHandler(error, c)
-        })
+          return errorHandler
+        }
       }
     }
   }
diff --git a/test-integration/apps.test.ts b/test-integration/apps.test.ts
index 938e193..01d7f13 100644
--- a/test-integration/apps.test.ts
+++ b/test-integration/apps.test.ts
@@ -158,6 +158,26 @@ describe('Basic', () => {
         method: 'GET',
         handler: expect.any(Function),
       },
+      {
+        path: '/directory/throw_error',
+        method: 'GET',
+        handler: expect.any(Function),
+      },
+      {
+        path: '/directory/throw_error',
+        method: 'GET',
+        handler: expect.any(Function),
+      },
+      {
+        path: '/directory/sub/throw_error',
+        method: 'GET',
+        handler: expect.any(Function),
+      },
+      {
+        path: '/directory/sub/throw_error',
+        method: 'GET',
+        handler: expect.any(Function),
+      },
       {
         path: '/fc',
         method: 'GET',
@@ -264,7 +284,7 @@ describe('With preserved', () => {
     eager: true,
   })
 
-  const ERROR = import.meta.glob('../mocks/app/routes/_error.tsx', {
+  const ERROR = import.meta.glob('../mocks/app/routes/**/_error.tsx', {
     eager: true,
   })
 
@@ -378,6 +398,22 @@ describe('With preserved', () => {
       '<!DOCTYPE html><html><head><title>Internal Server Error</title></head><body><h1>Custom Error Message: Foo</h1></body></html>'
     )
   })
+
+  it('Should return 500 response - /directory/throw_error', async () => {
+    const res = await app.request('/directory/throw_error')
+    expect(res.status).toBe(500)
+    expect(await res.text()).toBe(
+      '<!DOCTYPE html><html><head><title></title></head><body><h1>Custom Error in /directory: Foo</h1></body></html>'
+    )
+  })
+
+  it('Should return 500 response - /directory/sub/throw_error', async () => {
+    const res = await app.request('/directory/sub/throw_error')
+    expect(res.status).toBe(500)
+    expect(await res.text()).toBe(
+      '<!DOCTYPE html><html><head><title></title></head><body><h1>Custom Error in /directory: Foo</h1></body></html>'
+    )
+  })
 })
 
 describe('API', () => {