diff --git a/.github/CODEOWNERS b/.github/CODEOWNERS
new file mode 100644
index 0000000..77092ae
--- /dev/null
+++ b/.github/CODEOWNERS
@@ -0,0 +1 @@
+*  @nh13
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
new file mode 100644
index 0000000..0e8fdf3
--- /dev/null
+++ b/.github/workflows/ci.yml
@@ -0,0 +1,90 @@
+name: CI
+
+on: push
+env:
+  POETRY_VERSION: 1.8
+
+jobs:
+  testing:
+    runs-on: ubuntu-24.04
+    strategy:
+      matrix:
+        PYTHON_VERSION: ["3.9", "3.10", "3.11", "3.12"]
+    steps:
+    - uses: actions/checkout@v4
+    - uses: actions/checkout@v4
+      with:
+        repository: "lh3/bwa"
+        path: "bwa"
+    - name: Set up Python ${{matrix.PYTHON_VERSION}}
+      uses: actions/setup-python@v1
+      with:
+        python-version: ${{matrix.PYTHON_VERSION}}
+
+    - name: Get full Python version
+      id: full-python-version
+      shell: bash
+      run: echo ::set-output name=version::$(python -c "import sys; print('-'.join(str(v) for v in sys.version_info))")
+
+    - name: Install poetry
+      shell: bash
+      run: |
+        python -m pip install --upgrade pip
+        pip install poetry==${{env.POETRY_VERSION}}
+
+    - name: Configure poetry
+      shell: bash
+      run: poetry config virtualenvs.in-project true
+
+    - name: Set up cache
+      uses: actions/cache@v2
+      id: cache
+      with:
+        path: .venv
+        key: venv-${{ runner.os }}-${{ steps.full-python-version.outputs.version }}-${{ hashFiles('**/poetry.lock') }}
+
+    - name: Ensure cache is healthy
+      if: steps.cache.outputs.cache-hit == 'true'
+      shell: bash
+      run: poetry run pip --version >/dev/null 2>&1 || rm -rf .venv
+
+    - name: Check that the lock file is up to date
+      shell: bash
+      run: |
+        poetry lock --check
+
+    - name: Install deps
+      shell: bash
+      run: |
+        poetry install
+
+    - name: Style checking
+      shell: bash
+      run: |
+        poetry run ruff format --check bwapy tests
+
+    - name: Run lint
+      shell: bash
+      run: |
+        poetry run ruff check bwapy tests
+
+    - name: Run mypy
+      shell: bash
+      run: |
+        poetry run mypy bwapy tests --config=pyproject.toml
+
+    - name: Run pytest
+      shell: bash
+      run: |
+        poetry run python -m pytest --cov=bwapy --cov-report=xml --cov-branch 
+
+    - name: Run docs
+      shell: bash
+      run: |
+        set -euo pipefail
+        poetry run mkdocs build --strict
+
+    - name: Upload code coverage
+      uses: codecov/codecov-action@v4.5.0
+      with:
+        token: ${{ secrets.CODECOV_TOKEN }}
diff --git a/.github/workflows/readthedocs.yaml b/.github/workflows/readthedocs.yaml
new file mode 100644
index 0000000..7625296
--- /dev/null
+++ b/.github/workflows/readthedocs.yaml
@@ -0,0 +1,20 @@
+name: readthedocs/actions
+on:
+  pull_request_target:
+    types:
+      - opened
+    # Execute this action only on PRs that touch
+    # documentation files.
+    paths:
+      - "docs/**"
+
+permissions:
+  pull-requests: write
+
+jobs:
+  documentation-links:
+    runs-on: ubuntu-24.04
+    steps:
+      - uses: readthedocs/actions/preview@v1
+        with:
+          project-slug: "bwapy"
diff --git a/.readthedocs.yml b/.readthedocs.yml
new file mode 100644
index 0000000..6aa5b09
--- /dev/null
+++ b/.readthedocs.yml
@@ -0,0 +1,12 @@
+version: 2
+build:
+  os: ubuntu-24.04
+  tools:
+    python: "3.12"
+  jobs:
+    post_install:
+      - pip install poetry==1.8.3
+      - poetry config virtualenvs.create false
+      - VIRTUAL_ENV=$READTHEDOCS_VIRTUALENV_PATH poetry install
+mkdocs:
+  configuration: mkdocs.yml
diff --git a/README.md b/README.md
index 3ef54b4..08b5b9a 100644
--- a/README.md
+++ b/README.md
@@ -1 +1,30 @@
+[![Language][language-badge]][language-link]
+[![Code Style][code-style-badge]][code-style-link]
+[![Type Checked][type-checking-badge]][type-checking-link]
+[![PEP8][pep-8-badge]][pep-8-link]
+[![Code Coverage][code-coverage-badge]][code-coverage-link]
+[![License][license-badge]][license-link]
+
+
+[language-badge]:       http://img.shields.io/badge/language-python-brightgreen.svg
+[language-link]:        http://www.python.org/
+[code-style-badge]:     https://img.shields.io/badge/code%20style-black-000000.svg
+[code-style-link]:      https://black.readthedocs.io/en/stable/
+[type-checking-badge]:  http://www.mypy-lang.org/static/mypy_badge.svg
+[type-checking-link]:   http://mypy-lang.org/
+[pep-8-badge]:          https://img.shields.io/badge/code%20style-pep8-brightgreen.svg
+[pep-8-link]:           https://www.python.org/dev/peps/pep-0008/
+[code-coverage-badge]:  https://codecov.io/gh/fulcrumgenomics/bwapy/branch/main/graph/badge.svg
+[code-coverage-link]:   https://codecov.io/gh/fulcrumgenomics/bwapy
+[license-badge]:        http://img.shields.io/badge/license-MIT-blue.svg
+[license-link]:         https://github.com/fulcrumgenomics/bwapy/blob/main/LICENSE
+
 # bwapy
+
+Python language bindings for [bwa][bwa-link]
+
+See documentation on [bwapy.readthedocs.org][rtd-link].
+
+
+[rtd-link]: http://bwapy.readthedocs.org/en/stable
+[bwa-link]: https://github.com/lh3/bwa].
diff --git a/build.py b/build.py
new file mode 100644
index 0000000..4d80270
--- /dev/null
+++ b/build.py
@@ -0,0 +1,112 @@
+from setuptools import Extension, Distribution
+from typing import List
+
+from Cython.Build import cythonize
+from Cython.Distutils.build_ext import new_build_ext as cython_build_ext
+import multiprocessing
+from pathlib import Path
+
+SOURCE_DIR = Path("bwapy")
+BUILD_DIR = Path("cython_build")
+compile_args = []
+link_args = []
+include_dirs = ["bwa"]
+libraries = ['m', 'z', 'pthread']
+library_dirs=['bwa']
+extra_objects = []  #glob.glob(os.path.join('bwa', '*.o'))
+h_files = []
+c_files = []
+for root_dir in ["bwa", "bwapy"]:
+    h_files.extend(str(x) for x in Path(root_dir).rglob("*.h"))
+    c_files.extend(str(x) for x in Path(root_dir).rglob("*.c") if x.name not in ['example.c', 'main.c'])
+
+extension_module = Extension(
+    name='bwapy.libbwapy',
+    sources=['bwapy/libbwapy.pyx'] + c_files,
+    depends=h_files,
+    extra_compile_args=compile_args,
+    extra_link_args=link_args,
+    extra_objects=extra_objects,
+    include_dirs=include_dirs,
+    language='c',
+    libraries=libraries,
+    library_dirs=library_dirs,
+)
+
+
+def cythonize_helper(extension_modules: List[Extension]) -> List[Extension]:
+    """Cythonize all Python extensions"""
+
+    return cythonize(
+        module_list=extension_modules,
+
+        # Don't build in source tree (this leaves behind .c files)
+        build_dir=BUILD_DIR,
+
+        # Don't generate an .html output file. Would contain source.
+        annotate=False,
+
+        # Parallelize our build
+        nthreads=multiprocessing.cpu_count() * 2,
+
+        # Tell Cython we're using Python 3. Becomes default in Cython 3
+        compiler_directives={"language_level": "3"},
+
+        # (Optional) Always rebuild, even if files untouched
+        force=True,
+    )
+
+CLASSIFIERS = '''
+Development Status :: 4 - Beta
+Intended Audience :: Science/Research
+Intended Audience :: Developers
+License :: OSI Approved
+Programming Language :: Python
+Topic :: Software Development
+Topic :: Scientific/Engineering
+Operating System :: POSIX
+Operating System :: Unix
+Operating System :: MacOS
+'''
+
+
+def build():
+    # Collect and cythonize all files
+    extension_modules = cythonize_helper([extension_module])
+
+    # Use Setuptools to collect files
+    distribution = Distribution({
+        "name": "bwapy",
+        'version': '0.0.1',  # FIXME
+        'description': 'Todo',  # FIXME
+        'long_description': 'FIXME',
+        'long_description_content_type': 'text/x-rst',
+        'author': 'Nils Homer',
+        'author_email': 'nils@fulcrumgenomics.com',
+        'license': 'MIT',
+        'platforms': ['POSIX', 'UNIX', 'MacOS'],
+        'classifiers': [_f for _f in CLASSIFIERS.split('\n') if _f],
+        'url': 'https://github.com/fulcrumgenomics/bwapy',
+        'packages': ['bwapy'],
+        'package_dirs': {'bwapy': 'bwapy'},
+        "ext_modules": extension_modules,
+        "cmdclass": {
+            "build_ext": cython_build_ext,
+        },
+    })
+
+    # Grab the build_ext command and copy all files back to source dir.
+    # Done so Poetry grabs the files during the next step in its build.
+    build_ext_cmd = distribution.get_command_obj("build_ext")
+    build_ext_cmd.ensure_finalized()
+    # Set the value to 1 for "inplace", with the goal to build extensions
+    # in build directory, and then copy all files back to the source dir
+    # (under the hood, "copy_extensions_to_source" will be called after
+    # building the extensions). This is done so Poetry grabs the files
+    # during the next step in its build.
+    build_ext_cmd.inplace = 1
+    build_ext_cmd.run()
+
+
+if __name__ == "__main__":
+    build()
diff --git a/bwapy.yml b/bwapy.yml
new file mode 100644
index 0000000..768f735
--- /dev/null
+++ b/bwapy.yml
@@ -0,0 +1,9 @@
+name: bwapy
+channels:
+  - defaults
+  - conda-forge
+  - bioconda
+dependencies:
+  - python=3.11
+  - cython=3.0.11
+  - pysam=0.22.1
diff --git a/bwapy/__init__.py b/bwapy/__init__.py
new file mode 100644
index 0000000..6fc987c
--- /dev/null
+++ b/bwapy/__init__.py
@@ -0,0 +1,4 @@
+import bwapy.libbwapy as libbwapy
+from bwapy.libbwapy import *  # noqa: F403
+
+__all__ = libbwapy
diff --git a/bwapy/libbwapy.pxd b/bwapy/libbwapy.pxd
new file mode 100644
index 0000000..ee5e6dd
--- /dev/null
+++ b/bwapy/libbwapy.pxd
@@ -0,0 +1,119 @@
+# cython: language_level=3
+
+from libc.stdint cimport uint8_t, uint64_t, uint16_t, uint32_t, int64_t, int32_t
+from libc.stdio cimport FILE
+
+cdef extern from "libbwapy_utils.h":
+    void bwa_cal_pac_pos_with_bwt(const bntseq_t *bns, int n_seqs, bwa_seq_t *seqs, int max_mm,
+                                  float fnr, bwt_t *bwt)
+
+cdef extern from "utils.h":
+    int err_fseek(FILE *stream, long offset, int whence)
+    size_t err_fread_noeof(void *ptr, size_t size, size_t nmemb, FILE *stream)
+
+cdef extern from "bntseq.h":
+    unsigned char nst_nt4_table[256]
+    int bns_cnt_ambi(const bntseq_t *bns, int64_t pos_f, int len, int *ref_id)
+
+cdef extern from "bwa.h":
+    char * bwa_idx_infer_prefix(const char * hint)
+
+cdef extern from "bwt.h":
+    ctypedef struct bwt_t:
+        int sa_intv
+
+    bwt_t *bwt_restore_bwt(const char *fn)
+    void bwt_restore_sa(const char *fn, bwt_t *bwt);
+    void bwt_destroy(bwt_t *bwt)
+
+cdef extern from "bwtaln.h":
+    int BWA_TYPE_NO_MATCH
+    int BWA_MODE_LOGGAP
+    int BWA_MODE_GAPE
+
+    int __cigar_op(uint16_t __cigar)
+    int __cigar_len(uint16_t __cigar)
+
+    ctypedef struct gap_opt_t:
+        int trim_qual
+        int s_mm
+        int s_gapo
+        int s_gape
+        int mode # bit 24-31 are the barcode length
+        int indel_end_skip
+        int max_del_occ
+        int max_entries
+        float fnr
+        int max_diff
+        int max_gapo
+        int max_gape
+        int max_seed_diff
+        int seed_len
+        int n_threads
+        int max_top2
+        int trim_qual
+        int sam
+        char *rg_line
+        int n_occ
+        int interactive_mode
+        int with_md
+
+    gap_opt_t *gap_init_opt()
+    void gap_print_opt(const gap_opt_t *opt)
+
+    void seq_reverse(int len, unsigned char *seq, int is_comp)
+
+    ctypedef struct bwt_aln1_t:
+        pass
+
+cdef extern from "bntseq.h":
+    ctypedef  struct bntann1_t:
+        int64_t offset
+        int32_t len
+        char *name
+
+    ctypedef struct bntseq_t:
+        int64_t l_pac
+        bntann1_t *anns
+        FILE * fp_pac
+
+    bntseq_t * bns_restore(const char * prefix)
+    void bns_destroy(bntseq_t *bns)
+
+cdef extern from "kseq.h":
+    ctypedef struct kstring_t:
+        char *s
+
+cdef extern from "bwase.h":
+    void bwa_aln2seq_core(int n_aln, const bwt_aln1_t *aln, bwa_seq_t *s, int set_main, int n_multi)
+    int64_t pos_end(const bwa_seq_t *p)
+    void bwa_refine_gapped(const bntseq_t *bns, int n_seqs, bwa_seq_t *seqs, unsigned char *_pacseq)
+    char *bwa_cal_md1(int n_cigar, uint16_t *cigar, int len, uint64_t pos, unsigned char *seq, uint64_t l_pac, unsigned char *pacseq, kstring_t *str, int *_nm)
+    void bwase_initialize()
+
+cdef extern from "bwtaln.h":
+    ctypedef struct bwa_seq_t:
+
+        char *name
+        uint8_t *seq
+        uint8_t *rseq
+        uint8_t *qual
+        uint32_t len
+        uint32_t strand
+        uint32_t type
+        int mapQ
+        int clip_len
+        bwt_aln1_t *aln
+        int n_aln
+        uint16_t pos
+        uint16_t *cigar
+        int n_cigar
+        int tid
+        uint32_t full_len
+        uint32_t nm
+        char *md
+
+
+    void bwa_free_read_seq(int n_seqs, bwa_seq_t *seqs)
+
+    void bwa_cal_sa_reg_gap(int tid, bwt_t *const bwt, int n_seqs, bwa_seq_t *seqs, const gap_opt_t *opt)
diff --git a/bwapy/libbwapy.pyi b/bwapy/libbwapy.pyi
new file mode 100644
index 0000000..977e360
--- /dev/null
+++ b/bwapy/libbwapy.pyi
@@ -0,0 +1,42 @@
+from pathlib import Path
+from typing import List
+
+from pysam import AlignedSegment
+from pysam import AlignmentHeader
+from pysam import FastxRecord
+
+class BwaAlnOptions:
+    max_hits: int
+    def __init__(self, max_hits: int = 3) -> None: ...
+
+class BwaAlnOptionsBuilder:
+    _options: BwaAlnOptions
+    def __init__(self, options: BwaAlnOptions | None = None) -> None: ...
+    def build(self) -> BwaAlnOptions: ...
+    def max_mismatches(self, value: int) -> BwaAlnOptionsBuilder: ...  # -n <int>
+    # def fnr(self, value: float) -> BwaOptionsBuilder: ... # -n <float>
+    def max_gap_opens(self, value: int) -> BwaAlnOptionsBuilder: ...  # -o <int>
+    def max_gap_extensions(self, value: int) -> BwaAlnOptionsBuilder: ...  # -e <int>
+    def min_indel_to_end_distance(self, value: int) -> BwaAlnOptionsBuilder: ...  # -i <int>
+    def max_occurences_for_extending_long_deletion(
+        self, value: int
+    ) -> BwaAlnOptionsBuilder: ...  # -d <int>
+    def seed_length(self, value: int) -> BwaAlnOptionsBuilder: ...  # -l <int>
+    def max_mismatches_in_seed(self, value: int) -> BwaAlnOptionsBuilder: ...  # -k <int>
+    def mismatch_penalty(self, value: int) -> BwaAlnOptionsBuilder: ...  # -M <int>
+    def gap_open_penalty(self, value: int) -> BwaAlnOptionsBuilder: ...  # -O <int>
+    def gap_extension_penalty(self, value: int) -> BwaAlnOptionsBuilder: ...  # -E <int>
+    def stop_at_max_best_hits(self, value: int) -> BwaAlnOptionsBuilder: ...  # -R <int>
+    def max_hits(self, value: int) -> BwaAlnOptionsBuilder: ...  # bwa samse -n <int>
+    def log_scaled_gap_penalty(self, value: bool = True) -> BwaAlnOptionsBuilder: ...  # -L
+
+ERROR_HANDLER: str
+TEXT_ENCODING: str
+
+class BwaIndex:
+    header: AlignmentHeader
+    def __init__(self, prefix: str | Path) -> None: ...
+
+class BwaAln:
+    def __init__(self, prefix: str | Path | None = None, index: BwaIndex | None = None) -> None: ...
+    def align(self, opt: BwaAlnOptions, queries: List[FastxRecord]) -> List[AlignedSegment]: ...
diff --git a/bwapy/libbwapy.pyx b/bwapy/libbwapy.pyx
new file mode 100644
index 0000000..97c4862
--- /dev/null
+++ b/bwapy/libbwapy.pyx
@@ -0,0 +1,356 @@
+# cython: language_level=3
+
+from pathlib import Path
+from typing import List
+
+from cpython cimport PyBytes_Check, PyUnicode_Check
+from libc.stdint cimport uint8_t
+from libc.stdio cimport SEEK_SET
+from libc.stdlib cimport calloc, free
+from libc.string cimport strncpy, strcat
+from pysam import FastxRecord, AlignmentFile, AlignedSegment
+
+__all__ = [
+    "BwaAlnOptions",
+    "BwaAlnOptionsBuilder",
+    "BwaIndex",
+    "BwaAln",
+]
+
+cdef class BwaAlnOptions:
+    """The container for options for [`BwaAln`][bwapy.BwaAln].
+
+    Use [`BwaAlnOptionsBuilder`][bwapy.BwaAlnOptionsBuilder] to use and set custom options.
+    """
+    cdef gap_opt_t * _delegate
+    _max_hits: int
+
+    def __init__(self, max_hits: int = 3):
+        self._max_hits = max_hits
+        self._cinit()
+
+    cdef _cinit(self):
+        self._delegate = gap_init_opt()
+
+    def __dealloc__(self):
+        free(self._delegate)
+
+
+cdef class BwaAlnOptionsBuilder:
+    """Builder for options for [`BwaAln`][bwapy.BwaAln]"""
+    _options: BwaAlnOptions
+
+    def __init__(self, options: BwaAlnOptions | None = None) -> None:
+        self._options: BwaAlnOptions = BwaAlnOptions() if options is None else options
+
+    def build(self) -> BwaAlnOptions:
+        return self._options
+
+    cdef max_mismatches(self, value: int):
+        """bwa aln -n <int>"""
+        self._options._delegate.s_mm = value
+        return self
+    #cdef fnr(self, value: float) -> BwaOptionsBuilder: ... # -n <float>
+    cdef max_gap_opens(self, value: int):
+        """bwa aln -o <int>"""
+        self._options._delegate.max_gapo = value
+        return self
+
+    cdef max_gap_extensions(self, value: int):
+        """bwa aln -e <int>"""
+        self._options._delegate.max_gape = value
+        if self._delegate.max_gape > 0:
+            self._delegate.mode &= ~BWA_MODE_GAPE
+        return self
+
+    cdef min_indel_to_end_distance(self, value: int):
+        """bwa aln -i <int>"""
+        self._options._delegate.indel_end_skip = value
+        return self
+
+    def max_occurences_for_extending_long_deletion(self, value: int):
+        """bwa aln -d <int>"""
+        self._options._delegate.max_del_occ = value
+        return self
+
+    def seed_length(self, value: int):
+        """bwa aln -l <int>"""
+        self._options._delegate.seed_len = value
+        return self
+
+    def max_mismatches_in_seed(self, value: int):
+        """bwa aln -k <int>"""
+        self._options._delegate.max_seed_diff = value
+        return self
+
+    def mismatch_penalty(self, value: int):
+        """bwa aln -M <int>"""
+        self._options._delegate.s_mm = value
+        return self
+
+    def gap_open_penalty(self, value: int):
+        """bwa aln -O <int>"""
+        self._options._delegate.s_gapo = value
+        return self
+
+    def gap_extension_penalty(self, value: int):
+        """bwa aln -E <int>"""
+        self._options._delegate.s_gape = value
+        return self
+
+    def stop_at_max_best_hits(self, value: int):
+        """bwa aln -R <int>"""
+        self._options._delegate.max_top2 = value
+        return self
+
+    def max_hits(self, value: int):
+        """bwa samse -n <int>"""
+        self._options._max_hits = value
+        return self
+
+    def log_scaled_gap_penalty(self, value: bool = True):
+        """bwa aln -L"""
+        if value:
+            self._options._delegate.mode |= BWA_MODE_LOGGAP
+        else:
+            self._options._delegate.mode &= ~BWA_MODE_LOGGAP
+        return self
+
+
+cdef str ERROR_HANDLER = 'strict'
+cdef str TEXT_ENCODING = 'utf-8'
+
+
+cdef bytes force_bytes(object s, encoding=None, errors=None):
+    """convert string or unicode object to bytes, assuming
+    utf8 encoding.
+    """
+    if s is None:
+        return None
+    elif PyBytes_Check(s):
+        return s
+    elif PyUnicode_Check(s):
+        return s.encode(encoding or TEXT_ENCODING, errors or ERROR_HANDLER)
+    else:
+        raise TypeError("Argument must be string, bytes or unicode.")
+
+
+
+cdef class BwaIndex:
+    """Contains the index and nucleotide sequence for Bwa"""
+    cdef bwt_t *_bwt
+    cdef bntseq_t *_bns
+    cdef public object header
+
+    def __init__(self, prefix: str | Path):
+        self._cinit(f"{prefix}")
+  
+    cdef _cinit(self, prefix):
+        cdef char *local_prefix
+
+        prefix = bwa_idx_infer_prefix(force_bytes(prefix))
+        if not prefix:
+            # FIXME: better error message
+            raise Exception("Could not find the index")
+
+        # the SAM header from the sequence dictionary
+        seq_dict = Path(prefix.decode("utf-8")).with_suffix(".dict")
+        # TODO: error message when seq_dict is missing?
+        with seq_dict.open("r") as fh:
+            with AlignmentFile(fh) as reader:
+                self.header = reader.header
+
+        # from bwaaln.c
+        local_prefix = <char*>calloc(sizeof(char), len(prefix) + 10)
+
+        # load BWT
+        strncpy(local_prefix, prefix, len(prefix))
+        strcat(local_prefix, force_bytes(".bwt"))
+        self._bwt = bwt_restore_bwt(local_prefix)
+
+        # load SA
+        local_prefix[len(prefix)] = b'\0'  # trim off .bwt first
+        strcat(local_prefix, force_bytes(".sa"))
+        bwt_restore_sa(local_prefix, self._bwt)
+
+        # load BNS
+        self._bns = bns_restore(prefix)
+
+    def __dealloc__(self):
+        bwt_destroy(self._bwt)
+        self._bwt = NULL
+        bns_destroy(self._bns)
+        self._bns = NULL
+
+
+cdef class BwaAln:
+    """The class to align reads with `bwa aln`."""
+
+    cdef BwaIndex _index
+    cdef unsigned char* _pacseq
+
+    def __init__(self, prefix: str | Path | None = None, index: BwaIndex | None = None):
+        if prefix is not None:
+            assert Path(prefix).exists()
+            self._index = BwaIndex(prefix=prefix)
+        elif index is not None:
+            self._index = index
+        else:
+            raise Exception("Either prefix or index must be given")
+
+        bwase_initialize()
+
+        # initialize the packed binary reference sequence
+        self._pacseq = <unsigned char*>calloc(self._index._bns.l_pac//4+1, 1)
+        err_fseek(self._index._bns.fp_pac, 0, SEEK_SET)
+        err_fread_noeof(self._pacseq, 1, self._index._bns.l_pac//4+1, self._index._bns.fp_pac)
+
+    # TODO: a list of records...
+    def align(self, opt: BwaAlnOptions, queries: List[FastxRecord]) -> List[AlignedSegment]:
+        """Align one or more queries with `bwa aln`.
+
+        Args:
+            opt: the alignment options
+            queries: the queries to align
+
+        Returns:
+            one alignment per query
+        """
+        return self._calign(opt,  queries)
+
+    cdef _copy_seq(self, q: FastxRecord, bwa_seq_t* s):
+        seq_len = len(q.sequence)
+        s.len = seq_len
+        s.clip_len = seq_len
+        s.full_len = seq_len
+        s.seq = <uint8_t *> calloc(sizeof(uint8_t), seq_len + 1)
+        s.rseq = <uint8_t *> calloc(sizeof(uint8_t), seq_len + 1)
+        s.qual = <uint8_t *> calloc(sizeof(uint8_t), seq_len + 1)
+
+        # use seq_reverse from bwaseqio.c
+        for i, base in enumerate(q.sequence):
+            s.seq[i] = nst_nt4_table[ord(base)]
+            s.qual[i] = 40  # FIXME: parameterize
+        s.seq[seq_len] = b'\0'
+        s.qual[seq_len] = b'\0'
+        seq_reverse(seq_len, s.seq,
+                    0)  #  // *IMPORTANT*: will be reversed back in bwa_refine_gapped()
+        seq_reverse(seq_len, s.rseq, 0)
+
+        s.name = <char *> calloc(sizeof(char), len(q.name) + 1)
+        strncpy(s.name, force_bytes(q.name), len(q.name))
+        s.name[len(q.name)] = b'\0'
+
+    cdef _build_alignment(self, query: FastxRecord, bwa_seq_t *seq, kstring_t *kstr):
+        cdef int reference_id
+        cdef int nm
+
+        # make a default, unmapped, empty record
+        rec = AlignedSegment(header=self._index.header)
+        rec.query_name = query.name
+        rec.reference_id = -1
+        rec.reference_start = -1
+        rec.mapping_quality = 0
+        rec.is_paired = False
+        rec.is_read1 = True
+        rec.is_read2 = False
+        rec.is_qcfail = False
+        rec.is_duplicate = False
+        rec.is_secondary = False
+        rec.is_supplementary = False
+        rec.is_unmapped = True
+        rec.query_sequence = query.sequence
+        rec.query_qualities = query.quality
+
+        if seq.type == BWA_TYPE_NO_MATCH:  # unmapped read
+            # TODO: custom bwa tags: RG, BC, XC
+            return rec
+
+        ref_len_in_alignment = pos_end(seq) - seq.pos
+
+        # if on the reverse strand, reverse the query sequence and qualities
+        rec.query_sequence = query.sequence[::-1]
+        if query.quality is not None:
+            rec.query_qualities = query.quality[::-1]
+
+        # reference id
+        nn = bns_cnt_ambi(self._index._bns, seq.pos, ref_len_in_alignment, &reference_id)
+        rec.reference_id = reference_id
+
+        # make this unmapped if we map off the end of the contig
+        rec.is_unmapped = seq.pos + ref_len_in_alignment - self._index._bns.anns[
+            rec.reference_id].offset > self._index._bns.anns[rec.reference_id].len
+
+        # strand, reference start, and mapping quality
+        if seq.strand:
+            rec.is_reverse = True
+        rec.reference_start = seq.pos - self._index._bns.anns[rec.reference_id].offset + 1
+        rec.mapping_quality = seq.mapQ
+
+        # cigar
+        cigar = ""
+        if seq.cigar:
+            for j in range(seq.n_cigar):
+                cigar_len = __cigar_len(seq.cigar[j])
+                cigar_op = "MIDS"[__cigar_op(seq.cigar[j])]
+                cigar = f"{cigar}{cigar_len}{cigar_op}"
+        elif seq.type != BWA_TYPE_NO_MATCH:
+            cigar = f"{seq.len}M"
+        rec.cigarstring = cigar
+
+        # # tags
+        if seq.type != BWA_TYPE_NO_MATCH:
+            attrs = dict()
+            attrs["MD"] = f"{seq.md}"
+            attrs["NM"] = f"{seq.nm}"
+            rec.set_tags(list(attrs.items()))
+        # # TODO:the custom bwa tags: XT, NM, XN, SM, AM, X0, X1, XM, XO, XG, XA, HN
+
+        return rec
+
+    cdef _calign(self, opt: BwaAlnOptions, queries: List[FastxRecord]):
+        cdef bwa_seq_t* seqs
+        cdef bwa_seq_t* s
+        cdef char* s_char
+        cdef kstring_t* kstr
+
+        kstr = <kstring_t*>calloc(sizeof(kstring_t), 1)
+
+        # copy FastqProxy into bwa_seq_t
+        num_seqs = len(queries)
+        seqs = <bwa_seq_t*>calloc(sizeof(bwa_seq_t), num_seqs)
+        for i in range(num_seqs):
+            self._copy_seq(queries[i], &seqs[i])
+            seqs[i].tid = -1
+
+        # this is `bwa aln`, and the rest is `bwa samse`
+        bwa_cal_sa_reg_gap(0, self._index._bwt, num_seqs, seqs, opt._delegate)
+
+        # create the full alignment
+        for i in range(num_seqs):
+            s = &seqs[i]
+            # bwa_cal_sa_reg_gap frees name, seq, rseq, and qual, so add them back in again
+            self._copy_seq(queries[i], s)
+            bwa_aln2seq_core(s.n_aln, s.aln, s, 1, opt._max_hits)
+
+        # calculate the genomic position given the suffix array offsite
+        bwa_cal_pac_pos_with_bwt(self._index._bns, num_seqs, seqs, opt._delegate.max_diff, opt._delegate.fnr, self._index._bwt)
+
+        # TODO: can `_pacseq` be passed here?
+        # refine gapped alignment
+        bwa_refine_gapped(self._index._bns, num_seqs, seqs, NULL)
+
+        # create the AlignedSegment from FastxRecrod and bwa_seq_t.
+        recs = [
+            self._build_alignment(query=queries[i], seq=&seqs[i], kstr=kstr)
+            for i in range(num_seqs)
+        ]
+
+        bwa_free_read_seq(num_seqs, seqs)
+        free(kstr.s)
+        free(kstr)
+
+        return recs
+
+    def __dealloc__(self) -> None:
+        free(self._pacseq)
\ No newline at end of file
diff --git a/bwapy/libbwapy_utils.c b/bwapy/libbwapy_utils.c
new file mode 100644
index 0000000..b3c2d27
--- /dev/null
+++ b/bwapy/libbwapy_utils.c
@@ -0,0 +1,25 @@
+#include "bntseq.h"
+#include "bwt.h"
+#include "bwtaln.h"
+#include "kstring.h"
+#include "libbwapy_utils.h"
+#include "bwase.h"
+
+
+void bwa_cal_pac_pos_with_bwt(const bntseq_t *bns, int n_seqs, bwa_seq_t *seqs, int max_mm, float fnr, bwt_t *bwt)
+{
+    int i, j, strand, n_multi;
+    char str[1024];
+    for (i = 0; i != n_seqs; ++i) {
+        bwa_seq_t *p = &seqs[i];
+        bwa_cal_pac_pos_core(bns, bwt, p, max_mm, fnr);
+        for (j = n_multi = 0; j < p->n_multi; ++j) {
+            bwt_multi1_t *q = p->multi + j;
+            q->pos = bwa_sa2pos(bns, bwt, q->pos, p->len + q->ref_shift, &strand);
+            q->strand = strand;
+            if (q->pos != p->pos && q->pos != (bwtint_t)-1)
+                p->multi[n_multi++] = *q;
+        }
+        p->n_multi = n_multi;
+    }
+}
\ No newline at end of file
diff --git a/bwapy/libbwapy_utils.h b/bwapy/libbwapy_utils.h
new file mode 100644
index 0000000..cfd6769
--- /dev/null
+++ b/bwapy/libbwapy_utils.h
@@ -0,0 +1,8 @@
+#include "bntseq.h"
+#include "bwt.h"
+#include "bwtaln.h"
+#include "kstring.h"
+
+
+// Version of "bwa_cal_pac_pos" bwase.c that can use an already loaded forward suffix array (BWT)
+void bwa_cal_pac_pos_with_bwt(const bntseq_t *bns, int n_seqs, bwa_seq_t *seqs, int max_mm, float fnr, bwt_t *bwt);
diff --git a/bwapy/py.typed b/bwapy/py.typed
new file mode 100644
index 0000000..e69de29
diff --git a/codecov.yml b/codecov.yml
new file mode 100644
index 0000000..a901cc2
--- /dev/null
+++ b/codecov.yml
@@ -0,0 +1,2 @@
+ignore:
+  - "**/test_*.py"       # don't compute coverage of tests
diff --git a/docs/index.md b/docs/index.md
new file mode 100644
index 0000000..01ca5f5
--- /dev/null
+++ b/docs/index.md
@@ -0,0 +1,9 @@
+# bwapy
+
+Python bindings for BWA
+
+## Documentation Contents
+
+* [Installation](installation-and-developers-documentation.md)
+* [API](reference/bwapy/index.md)
+
diff --git a/docs/installation-and-developers-documentation.md b/docs/installation-and-developers-documentation.md
new file mode 100644
index 0000000..b69465a
--- /dev/null
+++ b/docs/installation-and-developers-documentation.md
@@ -0,0 +1,76 @@
+# Installation and Developer's Documentation
+
+## Recommended Installation
+
+The package `bwapy` requires cloning [`bwa`](https://github.com/lh3/bwa) into the source directory.
+
+```console
+git clone git@github.com:lh3/bwa.git
+```
+
+## Installation for Development and Release
+
+Install the Python build tool [`poetry`](https://python-poetry.org/docs/#installing-with-the-official-installer)
+
+Configure `poetry` to install into pre-existing virtual environments:
+
+```console
+poetry config virtualenvs.create false
+```
+
+```console
+poetry install
+```
+
+## Checking the Build
+
+Use `poetry` to test your code.
+
+```console
+poetry run pytest
+```
+
+Note that `poetry run pytest` will run `mypy` checks, `ruff` checks, `pytest` unit tests, and will provide a unit test coverage report.
+However, `pytest` will neither run the ruff formatter nor apply `ruff`'s automatic lint fixes, which can be done by calling `ruff` directly. 
+
+```console
+poetry run ruff format && poetry run ruff check --fix
+```
+
+## Building the Documentation
+
+Use `mkdocs` to build and serve the documentation.
+
+```console
+poetry run mkdocs build && poetry run mkdocs serve
+```
+
+## Creating a Release on PyPi
+
+1. Clone the repository recursively and ensure you are on the `main` (un-dirty) branch
+2. Checkout a new branch to prepare the library for release
+3. Bump the version of the library to the desired SemVer with `poetry version #.#.#`
+4. Commit the version bump changes with a Git commit message like `chore(release): bump to #.#.#`
+5. Push the commit to the upstream remote, open a PR, ensure tests pass, and seek reviews
+6. Squash merge the PR
+7. Tag the new commit on the main branch of the origin repository with the new SemVer
+
+> [!NOTE]
+> This project follows [Semantic Versioning](https://semver.org/).
+> In brief:
+> 
+> * `MAJOR` version when you make incompatible API changes
+> * `MINOR` version when you add functionality in a backwards compatible manner
+> * `PATCH` version when you make backwards compatible bug fixes
+
+GitHub Actions will take care of the remainder of the deployment and release process with:
+
+1. Unit tests will be run for safety-sake
+2. A source distribution will be built
+3. Multi-arch multi-Python binary distributions will be built
+4. Assets will be deployed to PyPi with the new SemVer
+5. A [Conventional Commit](https://www.conventionalcommits.org/en/v1.0.0/)-aware changelog will be drafted
+6. A GitHub release will be created with the new SemVer and the drafted changelog
+
+> [!IMPORTANT]
+> Consider editing the changelog if there are any errors or necessary enhancements.
diff --git a/docs/scripts/gen_ref_pages.py b/docs/scripts/gen_ref_pages.py
new file mode 100644
index 0000000..97b1411
--- /dev/null
+++ b/docs/scripts/gen_ref_pages.py
@@ -0,0 +1,49 @@
+"""Generate the code reference pages and navigation."""
+
+# Adapted from https://mkdocstrings.github.io/recipes/#bind-pages-to-sections-themselves
+
+from pathlib import Path
+
+import mkdocs_gen_files
+
+nav = mkdocs_gen_files.Nav()
+
+library_name = "bwapy"
+root = Path(__file__).parent.parent.parent
+src = root
+module_root = root / library_name
+
+assert module_root.exists(), (
+  f"module root does not exist: {module_root}\n"
+  f"Are you sure the library name '{library_name}' is correct?"
+)
+
+for path in sorted(module_root.rglob("*.py")):
+    module_path = path.relative_to(src).with_suffix("")
+    doc_path = path.relative_to(src).with_suffix(".md")
+    full_doc_path = Path("reference", doc_path)
+
+    parts = tuple(module_path.parts)
+
+    # ignore tests
+    if "tests" in parts:
+        continue
+
+    if parts[-1] == "__init__":
+        parts = parts[:-1]
+        doc_path = doc_path.with_name("index.md")
+        full_doc_path = full_doc_path.with_name("index.md")
+    elif parts[-1] == "__main__":
+        continue
+
+    nav[parts] = doc_path.as_posix()
+
+    with mkdocs_gen_files.open(full_doc_path, "w") as fd:
+        ident = ".".join(parts)
+        fd.write(f"::: {ident}")
+
+    #mkdocs_gen_files.set_edit_path(full_doc_path, Path("../") / path.relative_to(root))
+    mkdocs_gen_files.set_edit_path(full_doc_path, path.relative_to(root))
+
+with mkdocs_gen_files.open("reference/SUMMARY.md", "w") as nav_file:
+    nav_file.writelines(nav.build_literate_nav())
diff --git a/mkdocs.yml b/mkdocs.yml
new file mode 100644
index 0000000..33a05b0
--- /dev/null
+++ b/mkdocs.yml
@@ -0,0 +1,52 @@
+site_name: bwapy
+site_url: https://bwapy.readthedocs.io/en/latest/
+use_directory_urls: false
+theme:
+  name: material
+  highlightjs: true
+  hljs_languages:
+    - python
+  palette:
+    primary: teal
+  navstyle: dark
+  include_sidebar: true
+  collapse_navigation: false
+repo_url: https://github.com/fulcrumgenomics/bwapy
+plugins:
+  - autorefs:
+      resolve_closest: true
+  - include-markdown
+  - search
+  - gen-files:
+      scripts:
+        - docs/scripts/gen_ref_pages.py
+  - literate-nav:
+      nav_file: SUMMARY.md
+  - section-index
+  - mkdocstrings:
+      handlers:
+        python:
+          options:
+            docstring_section_style: table
+            docstring_style: google
+            group_by_category: true
+            separate_signature: true
+            show_category_heading: true
+            show_if_no_docstring: false
+            show_root_toc_entry: true
+            show_signature_annotations: true
+            signature_crossrefs: true
+            show_submodules: true
+  - table-reader
+markdown_extensions:
+  - pymdownx.highlight:
+      anchor_linenums: true
+      line_spans: __span
+      pygments_lang_class: true
+  - pymdownx.inlinehilite
+  - pymdownx.snippets
+  - pymdownx.superfences
+  - toc:
+      permalink: true
+exclude_docs: |
+  test_*.py
diff --git a/poetry.lock b/poetry.lock
new file mode 100644
index 0000000..2f27909
--- /dev/null
+++ b/poetry.lock
@@ -0,0 +1,1648 @@
+# This file is automatically @generated by Poetry 1.4.0 and should not be changed by hand.
+
+[[package]]
+name = "babel"
+version = "2.16.0"
+description = "Internationalization utilities"
+category = "dev"
+optional = false
+python-versions = ">=3.8"
+files = [
+    {file = "babel-2.16.0-py3-none-any.whl", hash = "sha256:368b5b98b37c06b7daf6696391c3240c938b37767d4584413e8438c5c435fa8b"},
+    {file = "babel-2.16.0.tar.gz", hash = "sha256:d1f3554ca26605fe173f3de0c65f750f5a42f924499bf134de6423582298e316"},
+]
+
+[package.extras]
+dev = ["freezegun (>=1.0,<2.0)", "pytest (>=6.0)", "pytest-cov"]
+
+[[package]]
+name = "bracex"
+version = "2.5.post1"
+description = "Bash style brace expander."
+category = "dev"
+optional = false
+python-versions = ">=3.8"
+files = [
+    {file = "bracex-2.5.post1-py3-none-any.whl", hash = "sha256:13e5732fec27828d6af308628285ad358047cec36801598368cb28bc631dbaf6"},
+    {file = "bracex-2.5.post1.tar.gz", hash = "sha256:12c50952415bfa773d2d9ccb8e79651b8cdb1f31a42f6091b804f6ba2b4a66b6"},
+]
+
+[[package]]
+name = "certifi"
+version = "2024.8.30"
+description = "Python package for providing Mozilla's CA Bundle."
+category = "dev"
+optional = false
+python-versions = ">=3.6"
+files = [
+    {file = "certifi-2024.8.30-py3-none-any.whl", hash = "sha256:922820b53db7a7257ffbda3f597266d435245903d80737e34f8a45ff3e3230d8"},
+    {file = "certifi-2024.8.30.tar.gz", hash = "sha256:bec941d2aa8195e248a60b31ff9f0558284cf01a52591ceda73ea9afffd69fd9"},
+]
+
+[[package]]
+name = "charset-normalizer"
+version = "3.4.0"
+description = "The Real First Universal Charset Detector. Open, modern and actively maintained alternative to Chardet."
+category = "dev"
+optional = false
+python-versions = ">=3.7.0"
+files = [
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diff --git a/pyproject.toml b/pyproject.toml
new file mode 100644
index 0000000..897a4b4
--- /dev/null
+++ b/pyproject.toml
@@ -0,0 +1,94 @@
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+readme = "README.md"
+homepage = "https://github.com/fulcrumgenomics/bwapy"
+repository = "https://github.com/fulcrumgenomics/bwapy"
+keywords = ["bioinformatics"]
+packages = [{ include = "bwapy" }]
+classifiers = [
+	"Development Status :: 3 - Alpha",
+	"Environment :: Console",
+	"Intended Audience :: Developers",
+	"Intended Audience :: Science/Research",
+	"License :: OSI Approved :: MIT License",
+	"Operating System :: OS Independent",
+	"Programming Language :: Python :: 3",
+	"Topic :: Scientific/Engineering :: Bio-Informatics",
+	"Topic :: Software Development :: Documentation",
+	"Topic :: Software Development :: Libraries :: Python Modules",
+]
+include = [
+	"LICENSE",
+	{ path = "bwapy/**/*.so", format = "wheel" },
+	{ path = "**/*.so", format = "wheel" },
+	{ path = "*.so", format = "wheel" },
+	#{ path = "*.so", format = "wheel" }
+]
+
+[tool.poetry.build]
+generate-setup-file = false
+script = "build.py"
+
+[tool.poetry.dependencies]
+python = ">=3.9.0,<4.0"
+pysam = ">=0.22.1"
+typing_extensions = { version = ">=3.7.4", python = "<3.12" }
+
+[tool.poetry.group.dev.dependencies]
+# dependencies for linting, style checking, and unit testing
+mypy = ">=1.7.0"
+pytest = ">=7.4.0"
+pytest-cov = ">=2.8.1"
+ruff = "0.4.8"
+setuptools = ">=68.0.0"
+
+[tool.poetry.group.docs.dependencies]
+# dependencies for building docs 
+mkdocs-autorefs = { version = ">=0.5.0" }
+mkdocs-include-markdown-plugin = { version = ">=6.0.1" }
+mkdocs-material = { version = ">=9.2.8" }
+mkdocs-table-reader-plugin = { version = ">=2.0.1" }
+mkdocs = { version = ">=1.5.2" }
+mkdocs-gen-files = { version = ">=0.5.0" }
+mkdocs-literate-nav = { version = ">=0.6.1" }
+mkdocs-section-index = { version = ">=0.3.9" }
+mkdocstrings-python = { version = ">=1.6.2" }
+mkdocstrings = { version = ">=0.23.0" }
+
+[build-system]
+requires = ["poetry-core>=1.0.0", "cython>=3.0.11", "setuptools>=75.1.0"]
+build-backend = "poetry.core.masonry.api"
+
+[tool.ruff]
+line-length    = 100
+target-version = "py38"
+output-format  = "full"
+
+[tool.ruff.lint]
+select    = ["C901", "B", "E", "F", "I", "W", "Q"]
+ignore    = ["E203", "E701"]
+unfixable = ["B"]
+
+[tool.ruff.lint.isort]
+force-single-line = true
+
+[tool.mypy]
+strict_optional             = false
+check_untyped_defs          = true
+disallow_incomplete_defs    = true
+disallow_untyped_calls      = true
+disallow_untyped_decorators = true
+disallow_untyped_defs       = true
+no_implicit_optional        = true
+warn_no_return              = true
+warn_redundant_casts        = true
+warn_return_any             = true
+warn_unreachable            = true
+warn_unused_configs         = true
+warn_unused_ignores         = true
+enable_error_code           = "ignore-without-code"
+exclude                     = ["site/", "docs/"]
diff --git a/tests/data/e_coli_k12.dict b/tests/data/e_coli_k12.dict
new file mode 100755
index 0000000..21006ce
--- /dev/null
+++ b/tests/data/e_coli_k12.dict
@@ -0,0 +1,2 @@
+@HD	VN:1.4	SO:unsorted
+@SQ	SN:U00096.2	LN:4639675	AS:e_coli_k12	M5:05dc7a37701cdc6bcf154344a227983d
diff --git a/tests/data/e_coli_k12.fasta b/tests/data/e_coli_k12.fasta
new file mode 100755
index 0000000..3147c84
--- /dev/null
+++ b/tests/data/e_coli_k12.fasta
@@ -0,0 +1,57997 @@
+>U00096.2  Escherichia coli K-12 MG1655 complete genome (4639675 bp)
+agcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactg
+gttacctgccgtgagtaaattaaaattttattgacttaggtcactaaatactttaaccaatataggcatagcgcacagac
+agataaaaattacagagtacacaacatccatgaaacgcattagcaccaccattaccaccaccatcaccattaccacaggt
+aacggtgcgggctgacgcgtacaggaaacacagaaaaaagcccgcacctgacagtgcgggctttttttttcgaccaaagg
+taacgaggtaacaaccatgcgagtgttgaagttcggcggtacatcagtggcaaatgcagaacgttttctgcgtgttgccg
+atattctggaaagcaatgccaggcaggggcaggtggccaccgtcctctctgcccccgccaaaatcaccaaccacctggtg
+gcgatgattgaaaaaaccattagcggccaggatgctttacccaatatcagcgatgccgaacgtatttttgccgaactttt
+gacgggactcgccgccgcccagccggggttcccgctggcgcaattgaaaactttcgtcgatcaggaatttgcccaaataa
+aacatgtcctgcatggcattagtttgttggggcagtgcccggatagcatcaacgctgcgctgatttgccgtggcgagaaa
+atgtcgatcgccattatggccggcgtattagaagcgcgcggtcacaacgttactgttatcgatccggtcgaaaaactgct
+ggcagtggggcattacctcgaatctaccgtcgatattgctgagtccacccgccgtattgcggcaagccgcattccggctg
+atcacatggtgctgatggcaggtttcaccgccggtaatgaaaaaggcgaactggtggtgcttggacgcaacggttccgac
+tactctgctgcggtgctggctgcctgtttacgcgccgattgttgcgagatttggacggacgttgacggggtctatacctg
+cgacccgcgtcaggtgcccgatgcgaggttgttgaagtcgatgtcctaccaggaagcgatggagctttcctacttcggcg
+ctaaagttcttcacccccgcaccattacccccatcgcccagttccagatcccttgcctgattaaaaataccggaaatcct
+caagcaccaggtacgctcattggtgccagccgtgatgaagacgaattaccggtcaagggcatttccaatctgaataacat
+ggcaatgttcagcgtttctggtccggggatgaaagggatggtcggcatggcggcgcgcgtctttgcagcgatgtcacgcg
+cccgtatttccgtggtgctgattacgcaatcatcttccgaatacagcatcagtttctgcgttccacaaagcgactgtgtg
+cgagctgaacgggcaatgcaggaagagttctacctggaactgaaagaaggcttactggagccgctggcagtgacggaacg
+gctggccattatctcggtggtaggtgatggtatgcgcaccttgcgtgggatctcggcgaaattctttgccgcactggccc
+gcgccaatatcaacattgtcgccattgctcagggatcttctgaacgctcaatctctgtcgtggtaaataacgatgatgcg
+accactggcgtgcgcgttactcatcagatgctgttcaataccgatcaggttatcgaagtgtttgtgattggcgtcggtgg
+cgttggcggtgcgctgctggagcaactgaagcgtcagcaaagctggctgaagaataaacatatcgacttacgtgtctgcg
+gtgttgccaactcgaaggctctgctcaccaatgtacatggccttaatctggaaaactggcaggaagaactggcgcaagcc
+aaagagccgtttaatctcgggcgcttaattcgcctcgtgaaagaatatcatctgctgaacccggtcattgttgactgcac
+ttccagccaggcagtggcggatcaatatgccgacttcctgcgcgaaggtttccacgttgtcacgccgaacaaaaaggcca
+acacctcgtcgatggattactaccatcagttgcgttatgcggcggaaaaatcgcggcgtaaattcctctatgacaccaac
+gttggggctggattaccggttattgagaacctgcaaaatctgctcaatgcaggtgatgaattgatgaagttctccggcat
+tctttctggttcgctttcttatatcttcggcaagttagacgaaggcatgagtttctccgaggcgaccacgctggcgcggg
+aaatgggttataccgaaccggacccgcgagatgatctttctggtatggatgtggcgcgtaaactattgattctcgctcgt
+gaaacgggacgtgaactggagctggcggatattgaaattgaacctgtgctgcccgcagagtttaacgccgagggtgatgt
+tgccgcttttatggcgaatctgtcacaactcgacgatctctttgccgcgcgcgtggcgaaggcccgtgatgaaggaaaag
+ttttgcgctatgttggcaatattgatgaagatggcgtctgccgcgtgaagattgccgaagtggatggtaatgatccgctg
+ttcaaagtgaaaaatggcgaaaacgccctggccttctatagccactattatcagccgctgccgttggtactgcgcggata
+tggtgcgggcaatgacgttacagctgccggtgtctttgctgatctgctacgtaccctctcatggaagttaggagtctgac
+atggttaaagtttatgccccggcttccagtgccaatatgagcgtcgggtttgatgtgctcggggcggcggtgacacctgt
+tgatggtgcattgctcggagatgtagtcacggttgaggcggcagagacattcagtctcaacaacctcggacgctttgccg
+ataagctgccgtcagaaccacgggaaaatatcgtttatcagtgctgggagcgtttttgccaggaactgggtaagcaaatt
+ccagtggcgatgaccctggaaaagaatatgccgatcggttcgggcttaggctccagtgcctgttcggtggtcgcggcgct
+gatggcgatgaatgaacactgcggcaagccgcttaatgacactcgtttgctggctttgatgggcgagctggaaggccgta
+tctccggcagcattcattacgacaacgtggcaccgtgttttctcggtggtatgcagttgatgatcgaagaaaacgacatc
+atcagccagcaagtgccagggtttgatgagtggctgtgggtgctggcgtatccggggattaaagtctcgacggcagaagc
+cagggctattttaccggcgcagtatcgccgccaggattgcattgcgcacgggcgacatctggcaggcttcattcacgcct
+gctattcccgtcagcctgagcttgccgcgaagctgatgaaagatgttatcgctgaaccctaccgtgaacggttactgcca
+ggcttccggcaggcgcggcaggcggtcgcggaaatcggcgcggtagcgagcggtatctccggctccggcccgaccttgtt
+cgctctgtgtgacaagccggaaaccgcccagcgcgttgccgactggttgggtaagaactacctgcaaaatcaggaaggtt
+ttgttcatatttgccggctggatacggcgggcgcacgagtactggaaaactaaatgaaactctacaatctgaaagatcac
+aacgagcaggtcagctttgcgcaagccgtaacccaggggttgggcaaaaatcaggggctgttttttccgcacgacctgcc
+ggaattcagcctgactgaaattgatgagatgctgaagctggattttgtcacccgcagtgcgaagatcctctcggcgttta
+ttggtgatgaaatcccacaggaaatcctggaagagcgcgtgcgcgcggcgtttgccttcccggctccggtcgccaatgtt
+gaaagcgatgtcggttgtctggaattgttccacgggccaacgctggcatttaaagatttcggcggtcgctttatggcaca
+aatgctgacccatattgcgggtgataagccagtgaccattctgaccgcgacctccggtgataccggagcggcagtggctc
+atgctttctacggtttaccgaatgtgaaagtggttatcctctatccacgaggcaaaatcagtccactgcaagaaaaactg
+ttctgtacattgggcggcaatatcgaaactgttgccatcgacggcgatttcgatgcctgtcaggcgctggtgaagcaggc
+gtttgatgatgaagaactgaaagtggcgctagggttaaactcggctaactcgattaacatcagccgtttgctggcgcaga
+tttgctactactttgaagctgttgcgcagctgccgcaggagacgcgcaaccagctggttgtctcggtgccaagcggaaac
+ttcggcgatttgacggcgggtctgctggcgaagtcactcggtctgccggtgaaacgttttattgctgcgaccaacgtgaa
+cgataccgtgccacgtttcctgcacgacggtcagtggtcacccaaagcgactcaggcgacgttatccaacgcgatggacg
+tgagtcagccgaacaactggccgcgtgtggaagagttgttccgccgcaaaatctggcaactgaaagagctgggttatgca
+gccgtggatgatgaaaccacgcaacagacaatgcgtgagttaaaagaactgggctacacttcggagccgcacgctgccgt
+agcttatcgtgcgctgcgtgatcagttgaatccaggcgaatatggcttgttcctcggcaccgcgcatccggcgaaattta
+aagagagcgtggaagcgattctcggtgaaacgttggatctgccaaaagagctggcagaacgtgctgatttacccttgctt
+tcacataatctgcccgccgattttgctgcgttgcgtaaattgatgatgaatcatcagtaaaatctattcattatctcaat
+caggccgggtttgcttttatgcagcccggcttttttatgaagaaattatggagaaaaatgacagggaaaaaggagaaatt
+ctcaataaatgcggtaacttagagattaggattgcggagaataacaaccgccgttctcatcgagtaatctccggatatcg
+acccataacgggcaatgataaaaggagtaacctgtgaaaaagatgcaatctatcgtactcgcactttccctggttctggt
+cgctcccatggcagcacaggctgcggaaattacgttagtcccgtcagtaaaattacagataggcgatcgtgataatcgtg
+gctattactgggatggaggtcactggcgcgaccacggctggtggaaacaacattatgaatggcgaggcaatcgctggcac
+ctacacggaccgccgccaccgccgcgccaccataagaaagctcctcatgatcatcacggcggtcatggtccaggcaaaca
+tcaccgctaaatgacaaatgccgggtaacaatccggcattcagcgcctgatgcgacgctggcgcgtcttatcaggcctac
+gttaattctgcaatatattgaatctgcatgcttttgtaggcaggataaggcgttcacgccgcatccggcattgactgcaa
+acttaacgctgctcgtagcgtttaaacaccagttcgccattgctggaggaatcttcatcaaagaagtaaccttcgctatt
+aaaaccagtcagttgctctggtttggtcagccgattttcaataatgaaacgactcatcagaccgcgtgctttcttagcgt
+agaagctgatgatcttaaatttgccgttcttctcatcgaggaacaccggcttgataatctcggcattcaatttcttcggc
+ttcaccgatttaaaatactcatctgacgccagattaatcaccacattatcgccttgtgctgcgagcgcctcgttcagctt
+gttggtgatgatatctccccagaattgatacagatctttccctcgggcattctcaagacggatccccatttccagacgat
+aaggctgcattaaatcgagcgggcggagtacgccatacaagccggaaagcattcgcaaatgctgttgggcaaaatcgaaa
+tcgtcttcgctgaaggtttcggcctgcaagccggtgtagacatcacctttaaacgccagaatcgcctggcgggcattcgc
+cggcgtgaaatctggctgccagtcatgaaagcgagcggcgttgatacccgccagtttgtcgctgatgcgcatcagcgtgc
+taatctgcggaggcgtcagtttccgcgcctcatggatcaactgctgggaattgtctaacagctccggcagcgtatagcgc
+gtggtggtcaacgggctttggtaatcaagcgttttcgcaggtgaaataagaatcagcatatccagtccttgcaggaaatt
+tatgccgactttagcaaaaaatgagaatgagttgatcgatagttgtgattactcctgcgaaacatcatcccacgcgtccg
+gagaaagctggcgaccgatatccggataacgcaatggatcaaacaccgggcgcacgccgagtttacgctggcgtagataa
+tcactggcaatggtatgaaccacaggcgagagcagtaaaatggcggtcaaattggtaatagccatgcaggccattatgat
+atctgccagttgccacatcagcggaaggcttagcaaggtgccgccgatgaccgttgcgaaggtgcagatccgcaaacacc
+agatcgctttagggttgttcaggcgtaaaaagaagagattgttttcggcataaatgtagttggcaacgatggagctgaag
+gcaaacagaataaccacaagggtaacaaactcagcaccccaggaacccattagcacccgcatcgccttctggataagctg
+aataccttccagcggcatgtaggttgtgccgttacccgccagtaatatcagcatggcgcttgccgtacagatgaccaggg
+tgtcgataaaaatgccaatcatctggacaatcccttgcgctgccggatgcggaggccaggacgccgctgccgctgccgcg
+tttggcgtcgaacccattcccgcctcattggaaaacatactgcgctgaaaaccgttagtaatcgcctggcttaaggtata
+tcccgccgcgccgcctgccgcttcctgccagccaaaagcactctcaaaaatagaccaaatgacgtggggaagttgcccga
+tattcattacgcaaattaccaggctggtcagtacccagattatcgccatcaacgggacaaagccctgcatgagccgggcg
+acgccatgaagaccgcgagtgattgccagcagagtaaagacagcgagaataatgcctgtcaccagcgggggaaaatcaaa
+agaaaaactcagggcgcgggcaacggcgttcgcttgaactccgctgaaaattatgccataggcgatgagcaaaaagacgg
+cgaacagaacgcccatccagcgcatccccagcccgcgcgccatataccatgccggtccgccacgaaactgcccattgacg
+tcacgttctttataaagttgtgccagagaacattcggcaaacgaggtcgccatgccgataaacgcggcaacccacatcca
+aaagacggctccaggtccaccggcggtaatagccagcgcaacgccggccaggttgccgctacccacgcgcgccgcaagac
+tggtacacaatgactgaaatgaggttaaaccgcctggctgtggatgaatgctatttttaagacttttgccaaactggcgg
+atgtagcgaaactgcacaaatccggtgcgaaaagtgaaccaacaacctgcgccgaagagcaggtaaatcattaccgatcc
+ccaaaggacgctgttaatgaaggagaaaaaatctggcatgcatatccctcttattgccggtcgcgatgactttcctgtgt
+aaacgttaccaattgtttaagaagtatatacgctacgaggtacttgataacttctgcgtagcatacatgaggttttgtat
+aaaaatggcgggcgatatcaacgcagtgtcagaaatccgaaacagtctcgcctggcgataaccgtcttgtcggcggttgc
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+acggacaaattgacctcccttcgtcagtacaccaccgtagtggccgacactggggacatcgcggcaatgaagctgtatca
+accgcaggatgccacaaccaacccttctctcattcttaacgcagcgcagattccggaataccgtaagttgattgatgatg
+ctgtcgcctgggcgaaacagcagagcaacgatcgcgcgcagcagatcgtggacgcgaccgacaaactggcagtaaatatt
+ggtctggaaatcctgaaactggttccgggccgtatctcaactgaagttgatgcgcgtctttcctatgacaccgaagcgtc
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+catcgatttaaacagttcgttgcgcatcagtacgctggagaggcgagttttgcctttttcattatgggtgagcaatcggg
+cgaaatttgccaactgttcctcactacaatgctgaagaaaatccagatctgaatcattcaggtaattaacattcattttt
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+aaaaggtgatttttgagacgcagcgtttattgtcgttatcgctgttaatgttgatccagtcagtggtttgcccttctttt
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+gcggcattgcaatggcgccgaggagtttatggtcgtttgcctgcgccgtgcagcacagcatcaggctaatcgccaggctg
+gcggaaatcgtaaaaacggatttcataaggattctcttagtgggaagaggtagggggatgaatacccactagtttactgc
+tgataaagagaagattcaggcacgtaatcttttctttttattacaattttttgatgaatgccttggctgcgattcattct
+ttatatgaataaaattgctgtcaattttacgtcttgtcctgccatatcgcgaaatttctgcgcaaaagcacaaaaaattt
+ttgcatctcccccttgatgacgtggtttacgaccccatttagtagtcaaccgcagtgagtgagtctgcaaaaaaatgaaa
+ttgggcagttgaaaccagacgtttcgcccctattacagactcacaaccacatgatgaccgaatatatagtggagacgttt
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+tagtgaattcacccaggcgtagcggcgggcgaataccaaaggcgctccattcaggagaacggcggtaaagtgcaggagtc
+tgggcaaactgcttcttgaatgcgcgggtaaatgtctgttgagagtcgaagcggtattgcagcgcgatgtccagaatcgg
+acgcgcagtcaggcgtagtgcgaccgccgatttcgacaaacgacgagcacgaatatacgcgccaatagcatggccagtga
+catctttaaacattctctgtaagtgccacttggaataacctgctttcgccgctacattgtcgagcgacaggggctgatcc
+agatgaccttccagccagattaaaaggtcgcgaataatgccggcctgatccataaaatatcctcatcctttcaacaacga
+gcacctgacatcaggtaattggataatagcattttttgctgttttagcattcagtgtttttttcttagtagagtatgttt
+tagggcttgacggaaataaaagtattgagattttgttcttaatcaatatgttatttaccgtgacgaactaattgctcgtg
+taatagataaaaatggtaacaatatgaaatacaagcatttgatcctgtctttaagcctgataatgctggggccattggct
+catgcagaagagattggttcggtcgacaccgtatttaaaatgatcggcccggatcacaaaattgttgtggaagcctttga
+tgatcccgatgtgaaaaatgtcacctgttatgtgagccgggcgaaaaccggtggtattaaagggggattgggtctggcgg
+aagatacctccgatgcggccatttcttgtcagcaagtcgggccgattgaactgtcggatcgtattaaaaacggcaaagct
+cagggcgaggtagtattcaaaaaacgcacgtccctggtctttaagtcgttacaggtcgtgcgcttttatgatgccaaacg
+caacgcgctcgcttatctggcttactccgacaaagttgtagaaggttcgccgaaaaacgcgattagcgcggttcctgtca
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+acgctggtctacatgttgcagcaggaaggttttgccgtcgaggtctttgagcgaggcttgccggtgctggataaagctcg
+caagcaggtacccgacgtcatgattctcgatgttggtctgccggatattagcggctttgaattgtgccgccagttactgg
+cgctccatccggcgttacctgtactgttcctgacggcccgaagtgaagaggtcgatcgcctgcttgggctggaaattggt
+gctgacgactacgtggctaaaccgttttcaccccgcgaagtgtgcgccagggtgcgcaccttactgcgtcgggtgaagaa
+gttctcgacgccgtctcccgtcatccgtattggacattttgaattgaatgaacccgcggcgcagatcagctggtttgaca
+cgccattagcgctgactcggtatgagtttttattgttgaagacgttactcaagtcaccgggccgcgtctggtcccgccag
+caactgatggatagcgtatgggaagatgcgcaggacacctacgatcgcaccgtcgatacccacattaaaacgctgcgtgc
+caagctgcgcgccatcaaccccgatctttcaccgattaatactcatcgcggcatgggatatagcctgaggggcctgtaat
+gcgtatcggcatgcggttgttgctgggctattttttactggtggcggtggcagcctggttcgtactggccatttttgtca
+aagaagttaaaccgggcgtgcgaagagcaacggaggggacgttgatcgacaccgcaacgttgctggcggagctggcgcgt
+cccgatttgctctctggggacccaacgcatgggcaactggcgcaggcgtttaatcagctacaacatcgcccgtttcgcgc
+caatatcggtggcattaacaaagtgcgcaatgaatatcatgtctatatgaccgatgcgcagggcaaagtattgttcgatt
+cggcaaataaagccgttggacaggattattcgcgctggaatgacgtctggctaacgttgcgtggtcagtatggtgcgcgc
+agcacgttgcaaaatcctgccgatcccgaaagttctgtgatgtatgttgccgcaccgattatggacggctcgcggcttat
+tggcgttttgagcgtaggcaaaccgaacgcggcgatggctccggtcattaagcgtagcgagcggcgaattttatgggcca
+gcgccattttgttggggattgcactggtgattggcgcaggcatggtttggtggatcaaccgctctattgcccggctcact
+cgctatgctgattccgtcactgacaataagcccgttcctctccccgatctcggtagtagcgagttgcgtaaactcgcgca
+ggcgctggaaagtatgcgcgtgaagctggaagggaaaaactatattgagcagtatgtttacgcattaactcatgagctaa
+aaagcccactggcggcgattcgtggagcggcggaaattttacgcgaaggtccgccgccggaagtggtggctcgttttact
+gacaacattctgacgcaaaatgcgcgtatgcaggcattggtagaaacgttactacgccaggcaagactggagaatcgtca
+ggaagtcgttctgactgctgttgatgtggcggcattattccgccgcgtcagcgaagcgcgcaccgtgcagttggcagaaa
+aaaaaatcactctgcatgttacgcccaccgaggttaacgttgctgctgaaccggcgttactggagcaggcgctgggaaat
+ttactggataacgccatcgattttacccccgagagcggttgcatcacgctaagcgccgaagtggatcaggaacacgtcac
+gcttaaggtgctggataccggtagtggtattcctgactacgcgctttcacgtatttttgaacgcttttactctttgcctc
+gtgcaaatgggcaaaaaagcagcggtctggggttggcgttcgtcagtgaggtcgcccgtttgtttaacggcgaagtcacg
+ctgcgcaacgtgcaggaaggtggcgtgctggcctcgcttcgacttcaccgtcacttcacatagcttcaaattcttcccac
+atagtcttcgtatcctgctgccattgcaaaggagaagactatgttgaaatcccccctgttctggaaaatgactagcctgt
+ttggtgcagtattgctgttgttgattccgataatgctgattcggcaggtgattgtcgaacgtgctgattaccgtagcgat
+gtggaagatgcgattcgccaaagtaccagcgggccgcaaaaactcgttgggccgctcatcgctattcctgtgaccgagct
+ttatacggtgcaggaagaggataaaaccgtggagcggaaacgaagttttatccatttttggttacctgagtcattgatgg
+ttgatggcaatcagaacgtggaagaacgcaagatagggatttataccggtcaggtctggcacagtgatttaacgttaaaa
+gccgatttcgatgtttcgcgtcttagcgaactcaacgcgccaaatatcaccttaggcaagccatttattgtgattagcgt
+cggggatgcgcgtggtattggtgtggtgaaagcgcctgaagttaacggaacggcgctgaccattgaacccggcaccgggt
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+acaacaaccggcgaaaagtgatgcaacggcagaccaacatcaactgcaagctttacgcgaacgagccatgacattgctga
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diff --git a/tests/data/e_coli_k12.fasta.amb b/tests/data/e_coli_k12.fasta.amb
new file mode 100755
index 0000000..e0164bf
--- /dev/null
+++ b/tests/data/e_coli_k12.fasta.amb
@@ -0,0 +1 @@
+4639675 1 0
diff --git a/tests/data/e_coli_k12.fasta.ann b/tests/data/e_coli_k12.fasta.ann
new file mode 100755
index 0000000..fdca4ee
--- /dev/null
+++ b/tests/data/e_coli_k12.fasta.ann
@@ -0,0 +1,3 @@
+4639675 1 11
+0 U00096.2  Escherichia coli K-12 MG1655 complete genome (4639675 bp)
+0 4639675 0
diff --git a/tests/data/e_coli_k12.fasta.bwt b/tests/data/e_coli_k12.fasta.bwt
new file mode 100755
index 0000000..b4e3804
Binary files /dev/null and b/tests/data/e_coli_k12.fasta.bwt differ
diff --git a/tests/data/e_coli_k12.fasta.fai b/tests/data/e_coli_k12.fasta.fai
new file mode 100755
index 0000000..c56e4bb
--- /dev/null
+++ b/tests/data/e_coli_k12.fasta.fai
@@ -0,0 +1 @@
+U00096.2	4639675	69	80	81
diff --git a/tests/data/e_coli_k12.fasta.pac b/tests/data/e_coli_k12.fasta.pac
new file mode 100755
index 0000000..38ba186
Binary files /dev/null and b/tests/data/e_coli_k12.fasta.pac differ
diff --git a/tests/data/e_coli_k12.fasta.sa b/tests/data/e_coli_k12.fasta.sa
new file mode 100755
index 0000000..5eebcde
Binary files /dev/null and b/tests/data/e_coli_k12.fasta.sa differ
diff --git a/tests/test_bwapy.py b/tests/test_bwapy.py
new file mode 100644
index 0000000..4e703ab
--- /dev/null
+++ b/tests/test_bwapy.py
@@ -0,0 +1,44 @@
+from pathlib import Path
+
+import pytest
+from pysam import FastxRecord
+
+from bwapy import BwaAln
+from bwapy import BwaAlnOptions
+from bwapy import BwaAlnOptionsBuilder
+from bwapy import BwaIndex
+
+
+@pytest.fixture()
+def ref_fasta() -> Path:
+    cur_dir = Path(__file__).parent
+    fasta: Path = cur_dir / "data" / "e_coli_k12.fasta"
+    return fasta
+
+
+def test_bwapy_options() -> None:
+    BwaAlnOptions()
+
+
+def test_bwapy_options_builder() -> None:
+    builder = BwaAlnOptionsBuilder()
+    builder.build()
+    # TODO: test setting individual options...
+
+
+def test_bwapy_index(ref_fasta: Path) -> None:
+    BwaIndex(prefix=ref_fasta)
+
+
+def test_bwapy(ref_fasta: Path) -> None:
+    opt = BwaAlnOptions()
+    bwa = BwaAln(prefix=ref_fasta)
+    sequence = "gttacctgccgtgagtaaattaaaattttattgacttaggtcactaaatactttaaccaatataggcatagcgcacagac"
+    fastqs = [FastxRecord(name="test", sequence=sequence)]
+
+    recs = bwa.align(opt=opt, queries=fastqs)
+    assert len(recs) == 1
+    rec = recs[0]
+    assert rec.query_name == "test"
+    assert rec.reference_start == 81
+    assert rec.cigarstring == "80M"