From 3ea67d5df8d06ef510953925ac113e99a055d17c Mon Sep 17 00:00:00 2001 From: kkobylin Date: Wed, 1 Jun 2022 13:21:12 +0200 Subject: [PATCH 1/2] Add feature_counts function to Spark SQL --- data/NA12878.slice.bam | Bin 0 -> 364043 bytes data/NA12878.slice.bam.bai | Bin 0 -> 504 bytes data/NA12878.slice.fasta | 2 + data/test.bed | 4 + .../sequila/apps/FeatureCounts.scala | 11 +- .../sequila/apps/FeatureCountsFunc.scala | 44 ++++++ .../IntervalTree/FeatureCountsPlan.scala | 147 ++++++++++++++++++ .../IntervalTreeJoinStrategyOptim.scala | 21 ++- .../optimizer/JoinOptimizerChromosome.scala | 29 +--- .../biodatageeks/sequila/utils/Columns.scala | 1 + .../sequila/utvf/FeatureCountsTemplate.scala | 32 ++++ .../utvf/ResolveTableValuedFunctionsSeq.scala | 15 +- 12 files changed, 267 insertions(+), 39 deletions(-) create mode 100644 data/NA12878.slice.bam create mode 100644 data/NA12878.slice.bam.bai create mode 100644 data/NA12878.slice.fasta create mode 100644 data/test.bed create mode 100644 src/main/scala/org/biodatageeks/sequila/apps/FeatureCountsFunc.scala create mode 100644 src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/FeatureCountsPlan.scala create mode 100644 src/main/scala/org/biodatageeks/sequila/utvf/FeatureCountsTemplate.scala diff --git a/data/NA12878.slice.bam b/data/NA12878.slice.bam new file mode 100644 index 0000000000000000000000000000000000000000..51c0c3969cebfac8299b3930e70a53247ae67fa2 GIT binary patch literal 364043 zcmZ77Q*b6c+W_F&w(V|h+qP}nw!O8zwQbw>Q*3R!|Gx8`{h7(pl}YZ&Npc5-F;HMY z|CJvIAYl;@pfJR%9JyFrE&YDJlnpcP2jb_^dtx@C0fOY`kMGl@(lggaDVt<@%AKaA z4Mxqn-H5H&tNY(qrdeXNwUMYO5$Ht2`hZ}tO<2oxR7~~Yo*H#yE(FRFK1X0^vf(~k zjW{2P+PeZYdL8sq3ICr;{~LpwpF5wQtk-}Gz<^BOTuX}gG%lzz4#7BrH zz(bz!dW%zD*TZo1`+ciC!CgM#LE*zQ@xf#-63?Rda zo;go+6OC%z9LAW08m0LcIGrYXDJ~_Oaj2z5oH3LQ8L3bxIXKLHMKBCCeJWDv714;g z(=V_GG>Kv3TS4p`C>-Llb{a)RS@hqMR<9z`4+8*n;caIaCXiK>_@J4%Vpc#Tt#&~{ z4$ZjAo>^#WX@O~|^%UI^1`aYhQV|U5IjaUzb}QA)pNe2vqzN?Ync;N_DDZ?D$b<)B 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CGATCCCTGACCCAGCACCGGGCACTGATGAGACAGCGGCTGTTTGAGGAGCCACCTCCCAGCCACCTCGGGGCCAGGGCCAGGGTGTGCAGCAccactgtacaatggggaaactggcccagagaggtgaggcagcttgcctggggtcacagagcaaggcaaaagcagcgctgggtacaagctcaAAACCATAGTGCCCAGGGCACTGCCGCTGCAGGCGCAGGCATCGCATCACACCAGTGTCTGCGTTCACAGCAGGCATCATCAGTAGCCTCCAGAGGCCTCAGGTCCAGTCTCTAAAAATATCTCAGGAGGCTGCAGTGGCTGACCATTGCCTTGGACCGCTCTTGGCAGTCGAAGAAGATTCTCCTGTCAGTTTGAGCTGGGTGAGCTTAGAGAGGAAAGCTCCACTATGGCTCCCAAACCAGGAAGGAGCCATAGCCCAGGCAGGAGGGCTGAGGACCTCTGGTGGCGGCCCAGGGCTTCCAGCATGTGCCCTAGGGGAAGCAGGGGCCAGCTGGCAAGAGCAGGGGGTGGGCAGAAAGCACCCGGTGGACTCAGGGCTGGAGGGGAGGAGGCGATCTTGCCCAAGGCCCTCCGACTGCAAGCTCCAGGGCCCGCTCACCTTGCTCCTGCTCCTTCTGCTGCTGCTTCTCCAGCTTTCGCTCCTTCATGCTGCGCAGCTTGGCCTTGCCGATGCCCCCAGCTTGGCGGATGGACTCTAGCAGAGTGGCCAGCCACCGGAGGGGTCAACCACTTCCCTGGGAGCTCCCTGGACTGGAGCCGGGAGGTGGGGAACAGGGCAAGGAGGAAAGGCTGCTCAGGCAGGGCTGGGGAAGCTTACTGTGTCCAAGAGCCTGCTGGGAGGGAAGTCACCTCCCCTCAAACGAGGAGCCCTGCGCTGGGGAGGCCGGACCTTTGGAGACTGTGTGTGGGGGCCTGGGCACTGACTTCTGCAACCACCTGAGCGCGGGCATCCTGTGTGCAGATACTCCCTGCTTCCTCTCTAGCCCCCACCCTGCAGAGCTGGACCCCTGAGCTAGCCATGCTCTGACAGTCTCAGTTGCACACACGAGCCAGCAGAGGGGTTTTGTGCCACTTCTGGATGCTAGGGTTACACTGGGAGACACAGCAGTGAAGCTGAAATGAAAAATGTGTTGCTGTAGTTTGTTATTAGACCCCTTCTTTCCATTGGTTTAATTAGGAATGGGGAACCCAGAGCCTCACTTGTTCAGGCTCCCTCTGCCCTAGAAGTGAGAAGTCCAGAGCTCTACAGTTTGAAAACCACTATTTTATGAACCAAGTAGAACAAGATATTTGAAATGGAAACTATTCAAAAAATTGAGAATTTCTGACCACTTAACAAACCCACAGAAAATCCACCCGAGTGCACTGAGCACGCCAGAAATCAGGTGGCCTCAAAGAGCTGCTCCCACCTGAAGGAGACGCGCTGCTGCTGCTGTCGTCCTGCCTGGCGCCTTGGCCTACAGGGGCCGCGGTTGAGGGTGGGAGTGGGGGTGCACTGGCCAGCACCTCAGGAGCtgggggtggtggtgggggcggtgggggtggtgttagtACCCCATCTTGTAGGTCTGAAACACAAAGTGTGGGGTGTCTAGGGAAGAAGGTGTGTGACCAGGGAGGTCCCCGGCCCAGCTCCCATCCCAGAACCCAGCTCACCTACCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCAATGTACATGAGGTCGTTGGCAATGCCGGGCAGGTCAGGCAGGTAGGATGGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCAAGATTAGGCAGGGACATGTGAGAGGTGACAGGGACCTGCAGGGGCAGCCAACAAGACCTTGTGTGCACCTCCCATGGGTGGAATAAGGGGCCCAACAGCCTTGACTGGAGAGGAGCTCTGGCAAGGCCCTGGGCCACTGCACCTGTCTCCACCTCTGTCCCACCCCTCCCACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACCTACAGAGGCGACATGGGGGTCAGGCAAGCTGACACCCGCTGTCCTGAGCCCATGTTCCTCTCCCACATCATCAGGGGCACAGCGTGCACTGTGGGGTCCCAGGCCTCCCGAGCCGAGCCACCCGTCACCCCCTGGCTCCTGGCCTATGTGCTGTACCTGTGTCTGATGCCCTGGGTCCCCACTAAGCCAGGCCGGGCCTCCCGCCCACACCCCTCGGCCCTGCCCTCTGGCCATACAGGTTCTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGCTGATGTTGCTGGGAAGACCCCCAAGTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGAGAGGGCCAGGATGGCCAAGGGATGGTGAATATTTGGTGCTGGGCCTAATCAGCTGCCATCCCATCCCAGTCAGCCTCCTCTGGGGGACAGAACCCTATGGTGGCCCCGGCTCCTCCCCAGTATCCAGTCCTCCTGGTGTGTGACAGGCTATATGCGCGGCCAGCAGACCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCTGCAGGCCAGGGTCCTGGGCGCCCGTGAAGATGGAGCCATATTCCTGCAGGCGCCCTGGAGCAGGGTACTTGGCACTGGAGAACACCTGTGGACACAGGGACAAGTCTGAGGGGGCCCCAAGAGGCTCAGAGGGCTAGGATTGCTTGGCAGGAGAGGGTGGAGTTGGAAGCCTGGGCGAGAAGAAAGCTCAAGGTACAGGTGGGCAGCAGGGCAGAGACTGGGCAGCCTCAGAGGCACGGGGAAATGGAGGGACTGCCCAGTAGCCTCAGGACACAGGGGTATGGGGACTACCTTGATGGCCTTCTTGCTGCCCTTGATCTTCTCAATCTTGGCCTGGGCCAAGGAGACCTTCTCTCCAATGGCCTGCACCTGGCTCCGGCTCTGCTCTACCTGCTGGGAGATCCTGCCATGGAGAAGATCACAGAGGCTGGGCTGCTCCCCACCCTCTGCACACCTCCTGCTTCTAACAGCAGAGCTGCCAGGCCAGGCCCTCAGGCAAGGGCTCTGAAGTCAGGGTCACCTACTTGCCAGGGCCGATCTTGGTGCCATCCAGGGGGCCTCTACAAGGATAATCTGACCTGCAGGGTCGAGGAGTTGACGGTGCTGAGTTCCCTGCACTCTCAGTAGGGACAGGCCCTATGCTGCCACCTGTACATGCTATCTGAAGGACAGCCTCCAGGGCACACAGAGGATGGTATTTACACATGCACACATGGCTACTGATGGGGCAAGCACTTCACAACCCCTCATGATCACGTGCAGCAGACAATGTGGCCTCTGCAGAGGGGGAACGGAGACCGGAGGCTGAGACTGGCAAGGCTGGACCTGAGTGTCGTCACCTAAATTCAGACGGGGAACTGCCCCTGCACATACTGAACGGCTCACTGAGCAAACCCCGAGTCCCGACCACCGCCTCAGTGTGGTCTAGCTcctcacctgcttccatcctccctggtgcggggtgggcccagtgatatcagctgcctgctgttccccagatgtgccaagtgcattcttgtgtgcttgcatctcatggaacgccatttccccagacatccctgtggctggctcctGATGCCCGAGGCCCAAGTGTCTGATGCTTTAAGGCACATCACCCCACTCATGCTTTTCCATGTTCTTTGGCCGCAGCAAGGCCGCTCTCACTGCAAAGTTAACTCTGATGCGTGTGTAACACAACATCCTCCTCCCAGTCGCCCCTGTAGCTCCCCTACCTCCAAGAGCCCAGCCCTTGCCCACAGGGCCACACTCCACGTGCAGAGCAGCCTCAGCACTCACCGGGCACGAGCGAGCCTGTGTGGTGCGCAGGGATGAGAAGGCAGAGGCGCGACTGGGGTTCATGAGGAAGGGCAGGAGGAGGGTGTGGGATGGTGGAGGGGTTTGAGAAGGCAGAGGCGCGACTGGGGTTCATGAGGAAAGGGAGGGGGAGGATGTGGGATGGTGGAGGGGCTGCAGACTCTGGGCTAGGGAAAGCTGGGATGTCTCTAAAGGTTGGAATGAATGGCCTAGAATCCGACCCAATAAGCCAAAGCCACTTCCACCAACGTTAGAAGGCCTTGGCCCCCAGAGAGCCAATTTCACAATCCAGAAGTCCCCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCAGGGGGCTCAGGCATGGCAGGGCTGGGAGTACCAGCAGGCACTCAAGCGGCTTAAGTGTTCCATGACAGACTGGTATGAAGGTGGCCACAATTCAGAAAGAAAAAAGAAGAGCACCATCTCCTTCCAGTGAGGAAGCGGGACCACCACCCAGCGTGTGCTCCATCTTTTCTGGCTGGGGAGAGGCCTTCATCTGCTGTAAAGGGTCCTCCAGCACAAGCTGTCTTAATTGACCCTAGTTCCCAGGGCAGCCTCGTTCTGCCTTGGGTGCTGACACGACCTTCGGTAGGTGCATAAGCTCTGCATTCGAGGTCcacaggggcagtgggagggaactgagactggggagggacaaaggctgctctgtcctggtgctcccacaaaggagaagggctgatcactcaaagttgcgaacaccaagctcaacaatgagccctggaaaatttctggaatggattattaaacagagagtctgtaagcacttagaaaaggccgcggtgagtcccaggggccagcactgctcgaaatgtacagcatttctctttgtaacaggattattagcctgctgtgcccggggaaaacatgcagcacagtgcatctcgagtcagcaggattttgacggcttctaacaaaatcttgtagacaagatggagctatgggggttggaggagagaacatataggaaaaatcagagccaaatgaaccacagccccaaagggcacagttgaacaatggactgattccagccttgcacggagggatctggcagagtccatccagttcattcaacacctggttagaaaactggggccagcacacaggggaagggtaagctggtttcatgatcgaatcaaggctcagacaatttttaaaggccagagggtagactgcaatcaccaagatgaaatttacaaggaacaaatgtgaagcccaacatttaggttttaaaaatcaagcgtataaatacagaaggtggagggaacttgctttagacacagttcaggtgaagaaagacctggaaacttctgttaactataagctcagtaggggctaaaagcatgttaatcggcataaaaaggcaatgagatcttaggGCACACAGCTCCCCGCCCCTCTTCTGCCCTTCATCCTTCTTTCAATCAGCAGGGACCGTGCACTCTCTTGGAGCCACCACAGAAAACAGAGGTGCATCCAGCACCACAGAAAACAGAGCCACCACAGAAAACAGAGGGTGACTGTCATCCCCTCCAGTCTCTGCACACTCCCAGCTGCAGCAGAGCAGGAGGAGAGAGCACAGCCTGCAATGCTAATTTGCCAGGAGCTCACCTGCCTGCGTCACTGGGCACAGACGCCAGTGAGGCCAGAGGCCGGGCTGTGCTGGGGCCTGAGCCGGGTGGTGGGGAGAGAGTCTCTCCCCTGCCCCTGTCTCTTCCGTGCAGGAGGAGCATGTTTAAGGGGACGGGTTCAAAGCTGGTCACATCCCCACCGAAAAAGCCCATGGACAACGAAAAGCCCACTAGCTTGTCCAGTGCCACAGGAGGGGCAAGTGGAGGAGGAGAGGTGGCGGTGCTCCCCACTCCACTGCCAGTCGTCACTGGCTCTCCCTTCCCTTCATCCTCGTTCCCTATCTGTCACCATTTCCTGTCGTCGTTTCCTCTGAATGTCTCACCCTGCCCTCCCTGCTTGCAAGTCCCCTGTCTGTAGCCTCACCCCTGTCGTATCCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATCCACTCTCTCTCCCTTCTTGTCCCTTCCGTGACGGATGCCTGAGGAACCTTCCCCAAACTCTTCTGTCCCATCCCTGCCCTGCTCAAAATCCAATCACAGCTCCCTAACACGCCTGAATCAACTTGAAGTCCTGTCTTGAGTAATCCGTGGGCCCTAACTCACTCATCCCAACTCTTCACTCACTGCCCTGCCCCACACCCTGCCAGGGAGCCTCCCGTGGCACCGTGGGGACACAAAGGAACCAGGGCAAAGCTCCCTCAGCCCCATTCAAAGAGGCCTGGCCCACAGGCTCACGGAAAGTCAGCCTCTCATGCCCCGAGAGCTGAGTGCAAGGGAGAGGCAGCGCTGTCTGTGCTTCCCATGCAGAAGCACCCCCCTCCCACCCCTGTGCAGGCCGGCCTTCGCGGCAGACCACCATACACCACGTTCCAAGCCACACTGAGGCCTCCCTCCAAGCCTGCAGCCCCCATTTCCAGACCCTGCCAGGGCAACCTGCATATCCACCTCCCTACCCTGCCCCCCTCTTCCAGGAGTCTGCCCTATGTGGAGTAAGCACgtggttttcctcttcagcaactatttcctttttactcaagcaatggccccatttcccttggggaatccatctctctcgcaggcttagtcccagagcttcaggtggggctgcccacagagctcctcagtctaagccaagtggtgtgtcatagtcccctggccccattaatggattctgggatagacatgaggaccaagccaggTGGGATGAGTGAGTGTGGCTTCTGGAGGAAGTGGGGACACAGGACAGCATTCTTTCCTGCTGGACCTGACCCTGTGTCATGTCACCTTGCTACCACGAGAGCATGGCCTGTCTGGGAATGCAGCCAGACCCAAAGAAGCAAACTGACATGGAAGGAAAGCAAAACCAGGCCCTGAGGACATCATTTTAGCCCTTACTCCGAAGGCTGCTCTACTGATTGGTTAATTTTTGCTTAGCTTGGTCTGGGGAGTTCTGACAGGCGTGCCACCAATTCTTACCGATTTCTCTCCACTCTAGACCCTGAGAAGCCCACGCGGTTCATGCTAGCAATTAACAATCAATCTCGCCCTATGTGTTCCCATTCCAGCCTCTAGGACACAGTGGCAGCCACATAATTGGTATCTCTTAAGGTCCAGCACGAGGTGGAGCACATGGTGGAGAGACAGATGCAGTGACCTGGAACCCAGGAGTGAGGGAGCCAGGACTCAGGCCCAAGGCTCCTGAGAGGCATCTGGCCCTCCCTGCGCTGTGCCAGCAGCTTGGAGAACCCACACTCAATGAACGCAGCACTCCACTACCCAGGAAATGCCTTCCTGCCCTCTCCTCATCCCATCCCTGGGCAGGGGACATGCAACTGTCTACAAGGTGCCAAGTACCAGGACAGGAAAGGAAAGACGCCAAAAATCCAGCGCTGCCCTCAGAGAAGGGCAACCACGCAGTCCCCATCTTGGCAAGGAAACACAATTTCCGAGGGAATGGTTTTGGCCTCCATTCTAAGTGCTGGACATGGGGTGGCCATAATCTGGAGCTGATGGCTCTTAAAGACCTGCATCCTCTTCCCTAGGTGTCCCTCGGGCACATTTAGCACAAAGATAAGCACAAAAGGTGCATCCAGCACTTTGTTACTATTGGTGGCAGGTTTATGAATGGCAACCAAAGGCAGTGTACGGGTCAAGATTATCAACAGGGAAGAGATagcatttcctgaaggcttcctaggtgccaggcactgttccattcctttgcatgttttgattaatttaatatttaaaataattctaccaggaagctaccattattaccacaacttcacaaatgagaacaccgaggcttagaggggttgggttgcccaaggttacagaggaagaaaacaggggagctggatctgagccaaggcatcaactccaaggtAACCCCTCAGTCACTTCACTGTGTGTCCCCTGGTTACTGGGACATTCTTGACAAACTCGGGGCAAGCCGGTGAGTCAGTGGGGGAGGACTTTCAGGAAGAGGTGGGTTCCCAGTTGGTGACAGAAGAGGAGGCTGCAAAGTGAAGGAGCAGGGGCTCCAGGTCTGGCGACAACCAGGGAAGGGACAGGGCAGGGATGGCTTGGACCACGAGAGGCACCTGAGTCAGGCAGTCACATACTTCCCACTGGGGTCTACCATGTGAGGCATGGTGTGGGATCCTGGGAAGGAGACCAAGCCTCATTTCAGTTTGCTTATGGCCAAAGACAGGACCTGTGTACCCGACAACCCCTGGGACCTTTACCAAAAAAAGAGCAAACACCATTCACTCACTCATGTTAGATAAACACTGAGTGAAGTCACTGGAGCCCAAGGACTGTGCGAGGTCAGCACTGCCAATACAAGAAGCTGCAGCCCTCCAGCTCGCctccctcaatggccactccgtgctccagccatgctggcttccttttaggtcctccacctccaggctgtagttcatgtgcttctttctggaatgttcttcccaacctacccactcaaccctcagactttaccataaatgtcatttcctcacgtctgccttccctgacctgagaccaagccaggCTTCCCATGACGAGCCTCACAGTACCCCATCTCCCCTGAACAGATGCAGTAATAACCTACATAACccggggccatgatctatggctttgaatcctggctctgtcactaggccaggtctctcagcccttctgtgcctcagtttcctcatctataaaatgagatgacggcagtgcctgctcatgaagtgtgagttaatgcactcaaatcaatggttgtgcacggtttatatgaatattagtgattaCAAAATATTATCAatagaccttgtcacaactgttattgaagaactaatcatctattgcttatttaggtctttctctcctgccagaatgtgcgctccaggtggagaggtatgttgccttatccgtggctggatatatagagattcccacactgccttgcacacgagcactgctgggtaaatatttgttggctgcaggaaAACGTGAAGGAATAGGCCCTCCAATGGGAGGAAAAGCATGAGTTGTGAGAGCAGAGCCACCACAGGAAACCAGGAGGCTAAGTGGGGTGGAAGGGAGTGAGCTCTCGGACTCCCAGGAGTAAAAGCTTCCAAGTTGGGCTCTCACTTCAGCCCCTCCCACACAGGGAAGCCAGATGGGTTCCCCAGGACCGGGATTCCCCAAGGGGGCTGCTCCCAGAGGGTGTGTTGCTGGGATTGCCCAGGACAGGGATGGCCCTCTCATCAGGTGGGGGTGAGTGGCAGCACCCACCTGCTGAAGATGTCTCCAGAGACCTTCTGCAGGTACTGCAGGGCATCCGCCATCTGCTGGACGGCCTCCTCTCGCCGCAGGTCTGGCTGGATGAAGGGCACGGCATAGGTCTGACCTGCCAGGGAGTGCTGCATCCTCACAGGAGTCATGGTGCCTGTGGGTCGGAGCCGGAGCGTCAGAGCCACCCACGACCACCGGCACGCCCCCACCACAGGGCAGCGTGGTGTTGAGACAACACAGCCCTCATCCCAACTATGCACATAGCTTCAGCCTGCACAGATAGGGGAGTAGGGGACAGAGCATTTGCTGAGAGGCCAGGAGCGCATAGATGGGACTCTGCTGATGCCTGCTGAGTGAATGAGGGAAAGGGCAGGGCCCGGGACTGGGGAATCTGTAGGGTCAATGGAGGAGTTCAGAGAAGGTGCAACATTTCTGACCCCCTACAAGGTGCTTGCTACCTGCCAGGCACCCTTTCCATACCTTGTCTCAGTTCAGCTCCCCACCTTGGATAAACAAGAAACCTTGGTTGCAGAGGAAAAAAGAGGCTGGAAACAAAGGGGTAGAAATGGGGTAGCAGGGGAGATTGCCTGATCAACTGCCAAATGGTACACAGTTCTGGAAAAGCACAAAAAATGTGCACACACGGGTTCTTCCCACTTTAACCCCTGAGGAATCTGAGGCCTGCTCCTGAAACAGACTGGGCAGTGGCTAGTGACTCTAGGTATAGGAGTATCCAGCCCTGCTCACCCAGGCTAGAGCTTAGGGGGACAAGAGGAAAGAGGTGCCTGTGGGGGTGGAGGACAGGAAGGAAAAACACTCCTGGAATTGCAAAGTGAGGGCAGAGTCTATTTATATTGGGTTTAATTAACTCCTCTCCCTGGTGCCACTAAAGCAGCAATCACACTGCAGACAGCACTGATTTGATTGGCAAGAGATGCACCAGGCAGAATATTAAGGGACCAGGCCCCTATAAATAGGCCTAATCACAGCCCCTCACTGGAAAATGGTAAGGAAGACATTAATCAGGCCTGGCACTGTGCCCTAGACCTGCTCCCCTAGGCACTACAGTGGGGCCCTTGGTTGCAACACAAGTAGGTAGGGATGGATGAGTGTGGCATGAAGGGCCTAGGAGATTTCACTTGGGTTTAAAATGCTGTGACCTTGAGTAAGTTGCCGTCTCTGAATCTGATCCTTTCGATTTCCCATTCTCCAAACTGAGAACTAGCACTGCTGAGACGTGGTTATTTCCAATAATAATTTGTATATTTTACATAACGCACCACACCAACATCTTCACCCAGTTGGAGCCTACTCCTTTGCTCCCGCTGCTGGCTTCCCCAGCCCTCCCTTCTGCCCTCCTCAGGCCAGCACTTTTCAGTGAGTTCCTCCTTTGCATACAGGCTTTCCAGATCTGTACTTGCCTTGAATACTCATCAGAGCCCAGGAGTTACTCCTCACCTCCCACTTATTTTTCCTCCCATCAAATAACTAAAGCATGGCCAGCTGATGCCCAGCCAACTGAGAAACCCAACCCTCTGAGACCAGCACACCCCTTTCAAGCATGTTCCTCCCTCCCCTTCTTTGTATTTATACTGATGCAAGTTTGCTGGCTGTcctaacttatttctgtgcctcagttctcccatatgtaagatcacaaagggggtaaagaTGCAAGATATTTCCTGTGCACATCTTCAGATGAATTTCTTGTTAGTGTGTGTGTGTTTGCTCACACATATGCGTGAAAGAAGAGTACATACACAGATCTCCTCAAAAAGGAGGCAGCAAGCCCGTTCAAGAATGGGACTGAATACACCTGATGAGTGGTTTACTTTCTGTCTGCAAACATCTACTGATCATCTGTTAGGTGCAGGCCATGATCACAACAAAGACGAATAAGACACTACACTAGCCAGGGAGAGTCTCAAAAACAACTAAACTCAAATTAAATTCATTCTACTCCAGTCATGGGTACAAAGCTAAGGAGTGACAAATCCCTCTTGGAGTTAGGGGAGTCAGGAAAAAGCTCTTAGCAGAATGTGTGCCTCTCggccgggcgcagcggctcacgcctgtaatcccagcactttgggaggcgaaggcaggcagatcacctgaggtcgggagttcgagaccagtctgaccaacatggtgaaactccatctctactaaaaatacaaaattagccaggcgtggtggtgcatgcctgtaatccccgctactcgggaggctgaggaaggagaatcacttgaaccaggaaggtggaggttgcagtgtgccaagatcgcgccatggcactccagcctaggcaacgagggtgaaCCAGGTCCAGGAAGAAGGTGCAAAGACAGCATTCCAGGTAAAAGAAACAGCTTGAACAAAAAGTGTGTAGGGGAACCGCAAGCGGTCTTGAGTGCTGAGGGTACAATCATCCTTGGGGAAGTACTAGAAGAAAGAATGATAAACAGAGGCCAGTTTGTTAAAAACACTCAAAATTAAAGCTAGGAGTTTGGACTTGTGGCAGGAATgaaatccttagacctgtgctgtccaatatggtagccaccaggcacatgcagccactgagcacttgaaatgtggatagtctgaattgagatgtgccataagtgtaaaatatgcaccaaatttcaaaggctagaaaaaaagaatgtaaaatatcttattattttatattgattacgtgctaaaataaccatatttgggatatactggattttaaaaatatatcactaatttcatctgtttctttttacttttAGAAATCACATATGTGACTTAAATATTTCTTTTCTTTTTCTTTCCTCTCACTCAGCGTCCTGTGATTCCAAAGAAATGAGTCTCTGCTGTTTTTGGGCAGCAGATATCCTAGAATGGACTCTGACCTAAGCATCAAAATTAATCATCATAACGTTATCATTTTATGGCCCCTTCTTCCTATATCTGGTAGCTTTTAAATGATGACCATGTAGATAATCTTTATTGTCCCTCTTTCAGCAGACGGTATTTTCTTATGCTACAGTATGACTGCTAATAATACCTACACATGTTAGAACCATTCTGACTCCTCAAGAatctcatttaactcttattatcagtgaatttatcatcatcccctattttacataaggaaatggggttagaaagaccaaataacattttttcaacatcaaaacactagcttgagatcaagcccagacttggatctgtcgtctgaattccaagctttttgttatttattgatatgttttgttgtTTTCATGCAATAATGCAAATCTTAGCCCAAACATTTTGTTAGTAGTACCAACTGTAAGTCACCTTATCTTCATACTTTGTCTTTATGTAAACCTAAATTAGATCTGTTTTTGATACTGAGGGAAAAACAAGGGAATctaacactaaccagcccgtagtgtgtggtcaacactttcgttactttagtatacatcaccccaattgtttgtcttcaccacacactttggagttaggtagtagtatctatttttacaaataagaaaacccaggcacaaaggggttgattagcAATTATCTTTTGAAAAGCCTGTAGTTGCTCATCTGAAGAAGTGACGGACCACCTCTTATTTAGTGGACAGACAGTAACTAGTTGAGAAGACAGGGGATTTTGTTGGCGGAAAAAAAAATTTATCAAAAGTCGTCTTCTATCAGGGAGTTTTATGAGAAACCCTAGCTCCTCAGTTCCACAGTGGGTAACTGTAATTCATTCTAGGTCTGCGATATTTCCTGCCTATCCATTTTGTTAACTCTTCAATGCATTCCACAAATACCTAAGTATTCTTTAATAATGGTGGTTTTTTTTTTTTTTTGCATCTATGAAGTTTTTTCAAATTCTTTTTAAGTGACAAAACTTGTACATGTGTATCGCTCAATATTTCTAGTCGACAGCACTGCTTTCGAGAATGTAAACCGTGCACTCCCAGGAAAATGCAGACACAGCACGCCTCTTTGGGACCGCGGTTTATACTTTCGAAGTGCTCGGAGCCCTTCCTCCAGACCGTTCTCCCACACCCCGCTCCAGGGTCTCTCCCGGAGTTACAAGCCTCGCTGTAGGCCCCGGGAACCCAACGCGGTGTCAGAGAAGTGGGGTCCCCTACGAGGGACCAGGAGCTCCGGGCGGGCAGCAGCTGCGGAAGAGCCGCGCGAGGCTTCCCAGAACCCGGCAGGGGCGGGAAGACGCAGGAGTGGGGAGGCGGAACCGGGACCCCGCAGAGCCCGGGTCCCTGCGCCCCACAAGCCTTGGCTTCCCTGCTAGGGCCGGGCAAGGCCGGGTGCAGGGCGCGGCTCCAGGGAGGAAGCTCCGGGGCGAGCCCAAGACGCCTCCCGGGCGGTCGGGGCCCAGCGGCGGCGTTCGCAGTGGAGCCGGGCACCGGGCAGCGGCCGCGGAACACCAGCTTGGCGCAGGCTTCTCGGTCAGGAACGGTCCCGGGCCTCCCGCCCGCCTCCCTCCAGCCCCTCCGGGTCCCCTACTTCGCCCCGCCAGGCCCCCACGACCCTACTTCCCGCGGCCCCGGACGCCTCCTCACCTGCGAGCCGCCCTCCCGGAAGCTCCCGCCGCCGCTTCCGCTCTGCCGGAGCCGCTGGGTCCTAGCCCCGCCGCCCCCAGTCCGCCCGCGCCTCCGGGTCCTAACGCCGCCGCTCGCCCTCCACTGCGCCCTCCCCGAGCGCGGCTCCAGGACCCCGTCGACCCGGAGCGCTGTCCTGTCGGGCCGAGTCGCGGGCCTGGGCACGGAACTCACGCTCACTCCGAGCTCCCGACGTGCACACGGCTCCCATGCGTTGTCTTCCGAGCGTCAGGCCGCCCCTACCCGTGCTTTCTGCTCTGCAGACCCTCTTCCTAGACCTCCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCTCAGGGCCAAGCTGTCCGCCAACCTCGGCTCCTCCGGGCAGCCCTCGCCCGGGGTGCGCCCCGGGGCAGGACCCCCAGCCCACGCCCAGGGCCCGCCCCTGCCCTCCAGCCCTACGCCTTGACCCGCTTTCCTGCGTCTCTCAGCCTACCTGACCTTGTCTTTACCTCTGTGGGCAGCTCCCTTGTGATCTGCTTAGTTCCCACCCCCCTTTAAGAATTCAATAGAGaagccagacgcaaaactacagatatcgtatgagtccagttttgtgaagtgcctagaatagtcaaaattcacagagacagaagcagtggtcgccaggaatggggaagcaaggcggagttgggcagctcgtgttcaatgggtagagtttcaggctggggtgatggaagggtgctggaaatgagtggtagtgatggcggcacaacagtgtgaatctacttaatcccactgaactgtatgctgaaaaatggtttagacggtgaattttaggttatgtatgttttaccacaatttttaaaaaGCTAGTGAAAAGCTGGTAAAAAGAAAGAAAAGAGGCTTTTTTAAAAAGTTAAATATATAAAAAGAGCATCATCAGTCCAAAGTCCAGCAGTTGTCCCTCCTGGAATCCGTTGGCTTGCCTCCGGCATTTTTGGCCCTTGCCTTTtagggttgccagattaaaagacaggatgcccagctagtttgaattttagataaacaacgaataatttcgtagcataaatatgtcccaagcttagtttgggacatacttatgctaaaaaacattattggttgtttatctgagattcagaattaagcattttatattttatttgctgcctctggccaccctaCTCTCTTCCTAACACTCTCTCCCTCTCCCAGTTTTGTCCGCCTTCCCTGCCTCCTCTTCTGGGGGAGTTAGATCGAGTTGTAACAAGAACATGCCACTGTCTCGCTGGCTGCAGCGTGTGGTCCCCTTACCAGAGGTAAAGAAGAGATGGATCTCCACTCAtgttgtagacagaatgtttatgtcctctccaaatgcttatgttgaaaccctaacccctaatgtgatggtatgtggagatgggcctttggtaggtaattacggttagatgaggtcatggggtggggccctcattatagatctggtaagaaaagagaGCATTGtctctgtgtctccctctctctctctctctctctctctcatttctctctatctcatttctctctctctcgctatctcatttttctctctctctctttctctcctctgtcttttcccaccaagtgaggatgcgaagagaaggtggctgtctgcaaaccaggaagagagccctcaccgggaacccgtccagctgccaccttgaacttggacttccaagcctccagaactgtgagggataaatgtatgattttaaagtcgcccagtgtgtggtattttgttttgactaatacaaCCTGAAAACATTTTCCCCTCACTCCACCTGAGCAATATCTGAGTGGCTTAAGGTACTCAGGACACAACAAAGGAGAAATGTCCCATGCACAAGGTGCACCCATGCCTGGGTAAAGCAGCCTGGCACAGAGGGAAGCACACAGGCTCAGggatctgctattcattctttgtgtgaccctgggcaagccatgaatggagcttcagtcaccccatttgtaatgggatttaattgtgcttgccctgcctccttttgagggctgtagagaaaagatgtcaaagtattttgtaATCTggctgggcgtggtggctcatgcctgtaatcctagcactttggtaggctgacgcgagaggactgcttgagcccaagagtttgagatcagcctgggcaatattgtgagattccatctctacaaaaataaaataaaatagccagtcatggtgtcacacacctgtagtcccagctacatgggaggctgaggcgggaggatcacttgagcttgggagatcgaggctgcagtgagctatgattgtaccactgcactccaggctgggcgacagagagagaccctgtctcagaaaaaaaaaaaaaaGTACTTTGTAATCTGTAAGGTTTATTTCAACACACACAAAAAAAGTGTATATGCTCCACGATGCCTGTGAATATACACACACACCACATCATATACCAAGCCTGGCTGTGTCTTCTCACAAATGCACTGCTAGGCACCACCCCCAGTTCTAGAATCACACCAGCCAGTTCACCCTCCAGATGGTTCACCCTCAACTTCATAAAAGTTCCCTACCTAATCTACTGACAGGCTCATCCCCGACCTAATTTTAAAGATTTCCTAGGAGCTGCAGTGGGAATCCTGGACCTCAGCCTGGACAAAGAACAGCTGCAGGTCATTCTCATGTGTGGACACAGAAGCTCTGCCTGCCTTTGCTGGCCAGCTGGGCTGAGCGGGCCTGGGAATTAAGGCTGCAGGGTTGGTCCCAGGCAGTCTTGCTGAAGCTTGCCACATCCCCCAGCCTCCTGGATTTGCCAGGATCCAAGAGCATGGACTTTAGGAATTCCTGGTGGAGGAGTGAAGAAAATGTGACAGGGTGTCCTAAGCCCCGATCTACAGGAAGAAAACTGGAAATAAGACTGAGGACTTAGTTTAAGATGTTCCTACTCAGCCTCTAGCTTTTGTGCTACAGTTCTGGGAACAGACTCCTCTCTCCTGAAAACCACTTCCCTCCGCAGCATTAGATTTCACCAAGATGTCTTGCTTGTGGGAAAGACTTCCAAGGATGCCTGGAGAGAGGAGGATGGAAATGTCCTGCTCTCTAAACAGATAGACAGATGCAGCCAGACAGAAAATAGTTTATCTTGCTGAGGTTTCTAATGTATTTGAAAGAGGCCTGGGTCTAGAAGTCTACCCAGAGGGCTCTGTGTTGTGCACGCAAAGATAAGAACCTTCCCTGTGGGAGTTCCAGAGCCAGTTTTCATAAACACCCATCGGTGACTGTGTTCAGAGTGAGTTCACACCATCCTGACCTGCCCTGAGTTAGACCTTACATGGTCTTCCTCCTCTAGGAAGCCTCTGCAGCCCAGGAACCTCCCCTTATCGGAAATGAACAGCATTTGAAGCTTCACCAGACAGACCAGACAGCTTAGCCCTCGTGTTGTGCCATGTGGGTTGTTCTCTGAGAGGcaggagagcatagtggttactaggaagggaaggactttgggactagactgcctcggctggagtcctctttctgcttcatagccacgtgatcctaggcatgttacctgtgcctcagttttcactctgtcaatatgtaataactgaatctgtctttgtggtgaggattcagtgagttaacatatttgaagtgcttaaaaATGAGGCTTGtgtccatagattaatgagtgaatacacaaatggtgatatggacatacagtggagtattagtcataaaaaggaaggcagagctgatccatggcaccatgtgacagaacctcaaaagcattaggttaagtggaagaagccagacacaggtcacctattgtgtaattccatttataggaaatatacagaatatgtaaatccgtggagaaagaaagccgatttccaggggctaaggggaggggagaatgggaagtggctgcttcatgggtacaaggtttcattttgagctgatgaaaatgttttggaactacatagagatagtgttggcacaacatggtgaatgtactgaatgccactgattgttcaatttaaaatggtcaaacttatatgaatttcacctccattaaaaaaaAAAAAAAAGgaccagatgtggttgctcacacccataatcccaacactttggaaAAAGGTGAAAGTTTTTTTTtctttttttttttatatacttaagttctagggtacatgtgcataatgtgcaggttggatacatagatatgcgtgtgccatgttggtttgctgcacccatcaacttgtcatttacattaggtatttcttctaatgctatccctcccccagccccccacccactgacaggccccagtgtatgatgttctctgccccatgtccaagcgttctcattgttcaattcccacctgtgagtgagaacatgcagtgtttggttttctgtctttgtgatagtttgctcagaatgatggtttccagcttcatccatgtccctgcaaaggacatgaactcatcctttttaatggctgcatagtatcccatggtatatatgtgccacattctcttaatccagtctgtcattgatggacatttgggttggttcaaagtctttgctattgtgaatactgccacaataaacatacatgtgcatgtgtctttatagtagcacgatttataatcctttgggtatatacccTAAGACctgggacgcatttaaagcagtgtgtaaagagacatttatagcactaaatgcccacaagagaCCTCTGCCTGAGAACGTGGGTTTCAGCCTAAGAGTTGTAATATGTGTGCCCATTCACAGGTGCTGCATCAGAGTCCCAGGTGGGAAGAAGGCAAGCATACACAAAAATGGTAAaaggcagaaaggagcccagtctcgttctttttaagaagttttcctaagaatctccacccagcgacttgctctcacatcttcttggccagcactggaccacacaactccttctagatacagaggagTCCTAGGATTCTATGAGAAAGAAGGGGAGGGTGGGCAAAGGGCAGCCAGCTGTGCAGCATCTGCTGGAGACACCTAACCCTTGGTGGAGGGGTTGTGGTGCTGGgagaaggctttctggacggtgtgacagcagagataaacttaaaggccaagtaggagttaccctggtgaagcagggcagggttacaagcattccagcaacatgaagcagcaGGAGtgttttaattaaaagaaggcagttgctgtaaccaactataaacaaataaaggcttaaacacaatggaagtttatttctcactaagggaacatccaaatccatgatactttaagtcagggacccaggttcctcccatctatggttctgccatcactaatctgggtcttccacaattgccgtgctccttggaggtgggaagagcaggcggaggacacgtgggaggttttagggacaagcctggaggcagcatgcgtcactcccatgcagagtccattggccaatgctggctccgatggccacatctcactgcaggggcagctgggaaatacagtctggctgtctacccaggaggaagagcagccagtttctgctgCTGATGATCAGGAGGTGGAGAAAATGTTCAGTCAGGCAGGGAGTGGGAATAGACAAGACCACAAGCAGCTTGGTGCCTCTGAAAGGGAGAGGGGTGGAGGGGAGACTAGAGAGGTGGGTAGGAATACTGGATTCCACTGACCACGTGCTGGATGTCACGCTTAGCCCTCCTGCTCTGTGCCGGGTTAGGCACCTGGTGTTTTACGTACATAATCTCAATTCTGTGAGGGCATCCGACCTGTGGGAAAAGAGCTGTTTGTTTCAAATGCCAGTCCTGCTTCCTAACAAGTGTTTAGAGCTTAATCGTGTTCAAAAtacatatacaatgtttaatacttacaagaatttggtggggaaaatattaccatctttcccttttgtgattggagaaaaatgaggctttgaagggtttaagaacttgcccaaggtcggccaggtgcagtggctcatgtctataatcccaacactttgggaggctgaggtgggaggatcgcttgaggccaggagttcaagaccagcctgagcaacatagtgagactttgtctctataaaaaataaataaataaataaaaaCAACTTGTCCAAGGTCAGACAGGCAGCCTCTTAGTAAGCACACATATCCTCTATATTATACTACCTCTCATGGAGGATCTCCTGTGTTCTACAAATAGTCTGGACTTGAGCCAGAATGTGTTATAATCCTGGGATCACGGCCAGTGGGCTTAGAAGAAGCCATCTCTTTCTCATGCCAAGATGAGGCTCCCCCAGATTTGCTCAGACTTACCTATAGTCAGCAGCATCGGGGGTCAGGAAAGACTTCACGAAGCCATAAATGCATCCTTCTCGGGGCAGCACCTGGCTCTCCCAGGTGAGAGAGGACTCCATTTTCACAGGCAGGCGTGGGAGCTTCAGCACCCATCTCTGGGCCCAGAATGACCCACTGGAGACCTTACAGCTCTCCTGTCACCCCCAATTCCTGCCCCCTCTGCAGCCTTGGAGGAGAATGGAGCTGAAGGGCCTGCCCTCTGTAGGGTGAGAAAGGGAGGCTAAAGCCTGGTGCCCACTGCCCTGGCTGCTCCGCATTGCAGGAGCTGCGCCCTTCCTTTCCTGGCACAGGGTCCACAGCCCCGAAACCCCGTTGTGTGGGAGCTGGGCACAGGGCAGCAGGACTAATCCTTGGAACAGCTCAGGGAGGATTATCCCAGCCACTGTCAGCAGCGGTGCAGCTGGCTCATTCCCATATAGGGGGAGGCCAGAGCCAGGGGCCTGCCACAAGTTGGAAGGCTGGGGAAGGGGAGGCCAGCAGAGGTGTCCTGGCTGTGGGTGGCTCTGAGGGGGCTCTCAGGGGTGGGGCTAAATCTCAGGGGCAGGATTATGTAAATCAAACCAATTCTAGCCACAGATTTAAAGTTTGGAAAAAAAAAAAAACCCAGCCTGGCGGAAAGAATTTAAATTATAAAAACTTAGAAGTATGGAATGTGAAATCATCCTGTAGGTGCTTATTTAACAACGAAATCATCCCGACACAATGAGCCATATGTGAAAAGTCATCCTTCCCCAACACATCCCCCAACAGGCACTCCTCAAGCCTCTCCCACCCAAGTGCTGGCATCCTCCCTGTCCTGCTTCACCTGAGACACCCCTTGTCTCATTAGACATGCAACTACGGGAGGGGTGACAGGAAGACAAGACACTATTTCCTCAGGCCCAGTTTGGTGTGGGGAGAAAGCCTCCTGATCCTGAAAGCAAGAATTTGACCAGAGCAGAAGTAATCAGTATGCAGATTGATTCTGTGGTATGTTAATGTTTATGCATAGATTATGAGGACCAGGTGAAAAGTGGGCCAGGGGAGCCAGATGTGTGTGTGAGTCATGGGTGGCTGAGATGAGGACAGGAGGGAAACTGGTTTGGAGGGTGCTGGCGATGGGGTGGGGGTGCCAGGAGGAAGGGAGGCTAGTTGTTTGAATGTCTGCATGAAAAAGCGGACGACAGCGGGGTCTGGGTGAATTCGGGCAACCATTTGGACCGTGGAGAAAACTGCCTGCGTGCGGCTGAGGACCTGCACTATTAATTTGTTTTTTAGCTAAGGCAAAGATAAATATAAAAACtgatactccacccagttaccagaaaacatttaggtatgtgtgagacaacttgggtatgtgaacctaccttttcaatgtaaattcagtgaaatctaagtgcagatcccatatttccaataaaaaggtaacatccaaactcagatgtcctatgagtataaaatacacaaagatcttctggacttagtatgaaaagggattttttttttgtcaggtacctcactagttatttttaaaataggattgcatgttgaaatgataatcttttggatatattgggttaaataaatttattattaaagttaatttcacttaaaaatgtttaatgtagctactagaaattttaaaattaagcatgttgctcaccttatgtttctattggacggctctCTCTAGATACAAAGGCTGCCAAGAGGGACCTCACTCTAGCTTCAGGGAGAAGAGAGGAATTAGCAAGGCCAAGCAGAGGCTCCTGAGGGCAGGGCCAAGGGCGGCTTGGTGGGGTGGGGATGGGATGCACAGAGATAACTCCAACCCTTAAGAAGGTGTTTCCTAGAGCAGGCTGTGACCTGTCAGTTTATATACTGAGGCTTAGGAGCCTCTTGGATGCCCCCAGATCTGCACCCCTGAATTGCCCTGTGCCCCTGCCGTCTTTGTTCCTGTGCTGGCATAGTGGTCTCACCTCCGGCAGtatcaccaccactgggcacaagcttctccagcacagcaactgtgtcttatttctccttgtactcccagtgttcacaccatgctgcactcacagaagactcttcgttgatattttgtggacagagagaatGCCTGTGAGAGTGGGCTGAAGTGTGCGTTGGGCTCCAGAGACCTTAAGGAGGGGAGACCAGGTCCTGAGTAAAGTTGAAGGGGAGGGGCTGAGTCCTGCTAGCCAGGAGTCTCATCCCCTGGGGAAGTTCCAGGGACCCCTCAGAAGTGCAAGGGGACGGTGTTAGTGTTAGTCCAGTAACACAGCCCAGAGCCTGCcttccacgtgggtttgacaggagcctcctaactgctcttctgcttccatttttgccccttcagtctattctcaacagggaagccagaggcatccttaaccatgtcagatcatgtggctcctcagctcaaagccTCATCTCAGAGGAAAGCTCTGGTCCCTTAGAAATGGCCCAAGTGGTGACAGACAGACTCTAAGGtgagcagactgttgctagatatctgggctcggaggactcgccactgctcaaaggcagtgaggattttcgcactagaagctggaggacagggatccttgttaggtaggagcagaaagcttagaaaagtggtctcctgcagttacgtggcaaacacatcatgtaagtgataaattgggtatgcagttgaggagatttccaagtaaaatgttgaggatgctgcctggtttcttcttactgcttataatatagtgtgagagaagagagataaattgagaaagagactggtttttaaactgttaaaattgaatcaggacttgatgattttgaaaattgtcagtctccccacatggaaaaagatgctgaaattaacaaatggcttctgagcatgtggcatagggtgtaactgtacagtcttttgtgattatgcataaagatcaaaggatgggagtagcaatgagtcacacagaggtctgttgcaagagattacaagggtgtaccatgcagaacctctccaccaaaccttagggcccttgggaagcttcagtgagttaccctgggggccatcttggcaggagctgaaggtagaaaggtagagtttatctctaaaagattcatgggtatggctcttgacaaatcgactatgagccccaccgaaacccacagaggacaggcaaagggtttgggaaagctgtttcacccacagtgctggcagattggtctgtaggggacagagtgcaaaatgaaagaagactgtcagagaccccaaactctgctgtcaagaagaaggctgataaaactacttggctgcaaacacgtggatctttcgtgagaaaagaaggatgacccagaggcagaagcccagaaggcagagccaagagacatggaatcttcccacatcttaaaacctgtttagggaacaccagcatctgtccagctggatttcagaaccaccattccttcatccttcccctgctgcctctttctgaacagcaatgtctcaagctttacccaccattgtgtgttgcatatgtagggggcagatagcttgtatctttagttttccagatcagaggaacatccaaagaaatctgttctacacctaaacccgatttagatgagattcgggactgtgagcatgaagggatctcaagaggggtgaatgtgttttgcatgcacaagggacaggagtcttggggacagaggacaggctgtggtggcagatactaaggtgacccccacaacccccacctctgccattcacacccttgaataatccccttctctggttgtaagcagaacctgtggcttgcttatgaaggaggcggtatatatgtgattcatgtactgatcatattgtataagatcactggctggatgcagtggctcgtgcctgtaatcccaacactttgggaggctgaggcgggtggatcacctgaggtcaggagttcgagaccaggctggccaacatggcaaaaccccgcctctactaaaaatacaaaaattagccaggcatagtggtgcacgcctgtaatcacagctactcaagaggctgaagcaggagaattgcttgaactcaggaggtggaggtggcagtgagccaagatcgtgccactgcactccagcctcagtgacagagcgagactctgtctcaaaaaataaataaataaaatgttaagatcataacctgtctttctggggactctctcttgacgcctttgaagaagcaggctgccatgttgcaagctgcctcatggaggggatcagctgcgaggagctaagagccccctccagtcgatgctcaccaggaagctgaggtcttgtgtccagcaccctgcatagaactgaatgctgccatgtgagcttggaagca diff --git a/data/test.bed b/data/test.bed new file mode 100644 index 00000000..7abe8ede --- /dev/null +++ b/data/test.bed @@ -0,0 +1,4 @@ +GeneId Chr Start End Strand +1 chr1 11 99 sss +2 chr1 222 257 a +3 chr1 800 2200 test \ No newline at end of file diff --git a/src/main/scala/org/biodatageeks/sequila/apps/FeatureCounts.scala b/src/main/scala/org/biodatageeks/sequila/apps/FeatureCounts.scala index 5f642d1a..f2a57549 100644 --- a/src/main/scala/org/biodatageeks/sequila/apps/FeatureCounts.scala +++ b/src/main/scala/org/biodatageeks/sequila/apps/FeatureCounts.scala @@ -6,8 +6,8 @@ import org.apache.spark.sql.SparkSession import org.biodatageeks.sequila.rangejoins.IntervalTree.IntervalTreeJoinStrategyOptim import org.biodatageeks.sequila.utils.{Columns, InternalParams} import org.rogach.scallop.ScallopConf -import org.seqdoop.hadoop_bam.{BAMInputFormat, SAMRecordWritable} import org.seqdoop.hadoop_bam.util.SAMHeaderReader +import org.seqdoop.hadoop_bam.{BAMInputFormat, SAMRecordWritable} object FeatureCounts { case class Region(contig:String, pos_start:Int, pos_end:Int) @@ -25,6 +25,7 @@ object FeatureCounts { val spark = SparkSession .builder() .appName("SeQuiLa-FC") + .config("spark.master", "local[4]") .getOrCreate() spark.sqlContext.setConf(InternalParams.useJoinOrder,"true") @@ -41,11 +42,11 @@ object FeatureCounts { count(*) AS Counts FROM reads JOIN targets |ON ( - | targets.Chr=reads.contigName + | targets.Chr=reads.contig | AND - | reads.end >= CAST(targets.Start AS INTEGER) + | reads.pos_end >= CAST(targets.Start AS INTEGER) | AND - | reads.start <= CAST(targets.End AS INTEGER) + | reads.pos_start <= CAST(targets.End AS INTEGER) |) |GROUP BY targets.GeneId,targets.Chr,targets.Start,targets.End,targets.Strand""".stripMargin spark @@ -70,7 +71,7 @@ object FeatureCounts { .option("delimiter", "\t") .csv(runConf.annotations()) targets - .withColumnRenamed("contigName", Columns.CONTIG) + .withColumnRenamed("contig", Columns.CONTIG) .createOrReplaceTempView("targets") spark.sql(query) diff --git a/src/main/scala/org/biodatageeks/sequila/apps/FeatureCountsFunc.scala b/src/main/scala/org/biodatageeks/sequila/apps/FeatureCountsFunc.scala new file mode 100644 index 00000000..9c218321 --- /dev/null +++ b/src/main/scala/org/biodatageeks/sequila/apps/FeatureCountsFunc.scala @@ -0,0 +1,44 @@ +package org.biodatageeks.sequila.apps + +import htsjdk.samtools.ValidationStringency +import org.apache.spark.sql.{SequilaSession, SparkSession} +import org.rogach.scallop.ScallopConf +import org.seqdoop.hadoop_bam.util.SAMHeaderReader + +object FeatureCountsFunc { + + class RunConf(args:Array[String]) extends ScallopConf(args){ + + val output = opt[String](required = true) + val annotations = opt[String](required = true) + val readsFile = trailArg[String](required = true) + verify() + } + + def main(args: Array[String]): Unit = { + val runConf = new RunConf(args) + val spark = SparkSession + .builder() + .appName("SeQuiLa-DoC") + .config("spark.master", "local[4]") + .getOrCreate() + + spark + .sparkContext + .setLogLevel("ERROR") + + spark + .sparkContext + .hadoopConfiguration.set(SAMHeaderReader.VALIDATION_STRINGENCY_PROPERTY, ValidationStringency.SILENT.toString) + + val ss = SequilaSession(spark) + + val query = s"Select fc.*, count(*) AS Counts " + + s" FROM feature_counts('${runConf.readsFile()}', '${runConf.annotations()}') fc " + + s" GROUP BY fc.sample_id, fc.contig, fc.pos_start, fc.pos_end, fc.strand, fc.length" + + ss.sql(query) + .coalesce(1) + .show() + } +} diff --git a/src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/FeatureCountsPlan.scala b/src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/FeatureCountsPlan.scala new file mode 100644 index 00000000..4f867028 --- /dev/null +++ b/src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/FeatureCountsPlan.scala @@ -0,0 +1,147 @@ +package org.biodatageeks.sequila.rangejoins.methods.IntervalTree + +import org.apache.spark.rdd.RDD +import org.apache.spark.sql.SequilaSession.logger +import org.apache.spark.sql.catalyst.InternalRow +import org.apache.spark.sql.catalyst.expressions.Attribute +import org.apache.spark.sql.execution.SparkPlan +import org.apache.spark.sql.{Row, SparkSession} +import org.apache.spark.unsafe.types.UTF8String +import org.apache.spark.util.SizeEstimator +import org.biodatageeks.sequila.datasources.BAM.{BAMFileReader, BDGAlignFileReaderWriter} +import org.biodatageeks.sequila.rangejoins.IntervalTree.Interval +import org.biodatageeks.sequila.rangejoins.optimizer.{JoinOptimizerChromosome, RangeJoinMethod} +import org.openjdk.jol.info.GraphLayout +import org.seqdoop.hadoop_bam.BAMBDGInputFormat + +import scala.collection.JavaConversions._ + +case class FeatureCountsPlan(spark: SparkSession, + readsPath: String, + genesPath: String, + output: Seq[Attribute], + minOverlap: Int, + maxGap: Int, + intervalHolderClassName: String) + extends SparkPlan with Serializable with BDGAlignFileReaderWriter[BAMBDGInputFormat] { + + override protected def doExecute(): RDD[InternalRow] = { + val reads = new BAMFileReader[BAMBDGInputFormat](spark, readsPath, None).readFile + val genesRdd = spark + .read + .option("header", "true") + .option("delimiter", "\t") + .csv(genesPath) + .rdd + + val optimizer = new JoinOptimizerChromosome(spark, genesRdd, genesRdd.count()) + logger.info(optimizer.debugInfo) + + if (optimizer.getRangeJoinMethod == RangeJoinMethod.JoinWithRowBroadcast) { + val localIntervals = { + if (maxGap != 0) { + genesRdd + .map(r => (r.getString(1), toInterval(r, maxGap), toInternalRow(r))) + } else { + genesRdd + .map(r => (r.getString(1), toInterval(r), toInternalRow(r))) + } + } + .collect() + + val intervalTree = { + val tree = new IntervalHolderChromosome[InternalRow](localIntervals, intervalHolderClassName) + try { + val treeSize = GraphLayout.parseInstance(tree).totalSize() + logger.info(s"Real broadcast size of the interval structure is ${treeSize} bytes") + } + catch { + case e@(_: NoClassDefFoundError | _: ExceptionInInitializerError) => + logger.error("Cannot get broadcast size, method ObjectSizeCalculator.getObjectSize not available falling back to Spark method") + val treeSize = SizeEstimator.estimate(tree) + logger.info(s"Real broadcast size of the interval structure is ${treeSize} bytes") + + } + spark.sparkContext.broadcast(tree) + } + + val v3 = reads.mapPartitions(readIterator => { + readIterator.map(read => { + intervalTree.value.getIntervalTreeByChromosome(read.getContig) match { + case Some(t) => { + val record = t.overlappers(read.getStart, read.getEnd) + if (minOverlap != 1) { + record + .filter(r => calcOverlap(read.getStart, read.getEnd, r.getStart, r.getEnd) >= minOverlap) + .flatMap(k => k.getValue) + } else { + record.flatMap(k => k.getValue) + } + } + case _ => Iterator.empty + } + }) + }) + .flatMap(r => r) + v3 + } else { + val genesRddWithIndex = genesRdd.zipWithIndex() + val localIntervals = genesRddWithIndex + .map(r=>(r._1.getString(1), toInterval(r._1) ,r._2)) + .collect() + + val intervalTree = { + val tree = new IntervalHolderChromosome[Long](localIntervals, intervalHolderClassName) + spark.sparkContext.broadcast(tree) + } + + val v3 = reads.mapPartitions(readIterator => { + readIterator.map(read => { + intervalTree.value.getIntervalTreeByChromosome(read.getContig) match { + case Some(t) => { + val record = t.overlappers(read.getStart, read.getEnd) + if (minOverlap != 1) { + record + .filter(r => calcOverlap(read.getStart, read.getEnd, r.getStart, r.getEnd) >= minOverlap) + .flatMap(k => k.getValue.map(s => (s, s))) + } else { + record.flatMap(k => k.getValue.map(s => (s, s))) + } + } + case _ => Iterator.empty + } + }) + }) + .flatMap(r => r) + + val intGenesRdd = genesRddWithIndex.map(r => (r._2, toInternalRow(r._1))) + val result = v3. + join(intGenesRdd) + .map(l => l._2._2) + result + } + } + + private def toInternalRow(r: Row): InternalRow = { + InternalRow.fromSeq(Seq( + UTF8String.fromString(r.getString(0)), //Sample + UTF8String.fromString(r.getString(1)), //Contig + r.getString(2).toInt, //Start + r.getString(3).toInt, //End + UTF8String.fromString(r.getString(4)), //Strand + r.getString(3).toInt - r.getString(2).toInt //Length + )) + } + + private def calcOverlap(start1: Int, end1: Int, start2: Int, end2: Int) = (math.min(end1, end2) - math.max(start1, start2) + 1) + + private def toInterval(r: Row) : Interval[Int] = { + Interval(r.getString(2).toInt, r.getString(3).toInt) + } + + private def toInterval(r: Row, maxGap: Int) : Interval[Int] = { + Interval(r.getString(2).toInt - maxGap, r.getString(3).toInt + maxGap) + } + + override def children: Seq[SparkPlan] = Nil +} diff --git a/src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/IntervalTreeJoinStrategyOptim.scala b/src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/IntervalTreeJoinStrategyOptim.scala index 4ee29e05..b071e986 100644 --- a/src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/IntervalTreeJoinStrategyOptim.scala +++ b/src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/IntervalTreeJoinStrategyOptim.scala @@ -1,15 +1,13 @@ package org.biodatageeks.sequila.rangejoins.IntervalTree -import org.biodatageeks.sequila.rangejoins.common.{ExtractRangeJoinKeys, ExtractRangeJoinKeysWithEquality} -import org.biodatageeks.sequila.rangejoins.methods.IntervalTree.IntervalTreeJoinOptimChromosome import org.apache.spark.sql.catalyst.expressions.PredicateHelper +import org.apache.spark.sql.catalyst.plans.logical.LogicalPlan import org.apache.spark.sql.execution.SparkPlan -import org.apache.spark.sql.{SparkSession, Strategy} -import org.apache.spark.sql.catalyst.plans.logical.{Filter, LogicalPlan} +import org.apache.spark.sql.{FeatureCountsTemplate, SparkSession, Strategy} +import org.biodatageeks.sequila.rangejoins.common.{ExtractRangeJoinKeys, ExtractRangeJoinKeysWithEquality} +import org.biodatageeks.sequila.rangejoins.methods.IntervalTree.{FeatureCountsPlan, IntervalTreeJoinOptimChromosome} import org.biodatageeks.sequila.utils.InternalParams -import scala.annotation.tailrec - /** * Created by marek on 27/01/2018. */ @@ -42,6 +40,17 @@ class IntervalTreeJoinStrategyOptim(spark: SparkSession) extends Strategy with S useJoinOrder.toBoolean, intervalHolderClassName) :: Nil } + case FeatureCountsTemplate(reads, genes, output) => + val minOverlap = spark.sqlContext.getConf(InternalParams.minOverlap,"1") + val maxGap = spark.sqlContext.getConf(InternalParams.maxGap,"0") + FeatureCountsPlan( + spark, + reads, + genes, + output, + minOverlap.toInt, + maxGap.toInt, + intervalHolderClassName) :: Nil case _ => Nil } diff --git a/src/main/scala/org/biodatageeks/sequila/rangejoins/optimizer/JoinOptimizerChromosome.scala b/src/main/scala/org/biodatageeks/sequila/rangejoins/optimizer/JoinOptimizerChromosome.scala index 665bc804..44998c77 100644 --- a/src/main/scala/org/biodatageeks/sequila/rangejoins/optimizer/JoinOptimizerChromosome.scala +++ b/src/main/scala/org/biodatageeks/sequila/rangejoins/optimizer/JoinOptimizerChromosome.scala @@ -1,29 +1,24 @@ package org.biodatageeks.sequila.rangejoins.optimizer import org.apache.log4j.Logger -import org.apache.spark.SparkContext import org.apache.spark.rdd.RDD import org.apache.spark.sql.SparkSession -import org.apache.spark.sql.catalyst.InternalRow -import org.apache.spark.sql.catalyst.expressions.GenericInternalRow import org.apache.spark.util.SizeEstimator -import org.biodatageeks.sequila.rangejoins.IntervalTree.{Interval, IntervalWithRow} import org.biodatageeks.sequila.rangejoins.optimizer.RangeJoinMethod.RangeJoinMethod import org.biodatageeks.sequila.utils.InternalParams import org.openjdk.jol.info.GraphLayout -class JoinOptimizerChromosome(spark: SparkSession, rdd: RDD[(String,Interval[Int],InternalRow)], rddCount : Long) { +class JoinOptimizerChromosome [T <: Object] (spark: SparkSession, rdd: RDD[T], rddCount : Long) { - val logger = Logger.getLogger(this.getClass.getCanonicalName) - val maxBroadcastSize = spark.sqlContext + val logger: Logger = Logger.getLogger(this.getClass.getCanonicalName) + val maxBroadcastSize: Double = spark.sqlContext .getConf(InternalParams.maxBroadCastSize,"0") match { case "0" => 0.1*scala.math.max((spark.sparkContext.getConf.getSizeAsBytes("spark.driver.memory","0")),1024*(1024*1024)) //defaults 128MB or 0.1 * Spark Driver's memory case _ => spark.sqlContext.getConf(InternalParams.maxBroadCastSize).toLong } - val estBroadcastSize = estimateBroadcastSize(rdd,rddCount) + val estBroadcastSize: Long = estimateBroadcastSize(rdd,rddCount) - - private def estimateBroadcastSize(rdd: RDD[(String,Interval[Int],InternalRow)], rddCount: Long): Long = { + private def estimateBroadcastSize(rdd: RDD[T], rddCount: Long): Long = { try{ (GraphLayout.parseInstance(rdd.first()).totalSize() * rddCount) } @@ -36,7 +31,7 @@ class JoinOptimizerChromosome(spark: SparkSession, rdd: RDD[(String,Interval[Int //FIXME: Do not know why the size ~10x the actual size is- Spark row representation or getObject size in bits??? } - def debugInfo = { + def debugInfo: String = { s""" |Estimated broadcast structure size is ~ ${math.rint(100*estBroadcastSize/1024.0)/100} kb |${InternalParams.maxBroadCastSize} is set to ${(maxBroadcastSize/1024).toInt} kb" @@ -44,24 +39,16 @@ class JoinOptimizerChromosome(spark: SparkSession, rdd: RDD[(String,Interval[Int """.stripMargin } - private def estimateRDDSizeSpark(rdd: RDD[(String,Interval[Int],InternalRow)]): Long = { - math.round(SizeEstimator.estimate(rdd)/1024.0) - } - /** * Choose range join method to use basic on estimated size of the underlying data struct for broadcast * @param rdd * @return */ - def getRangeJoinMethod : RangeJoinMethod ={ - - if (estimateBroadcastSize(rdd, rddCount) <= maxBroadcastSize) + def getRangeJoinMethod : RangeJoinMethod = { + if (estBroadcastSize <= maxBroadcastSize) RangeJoinMethod.JoinWithRowBroadcast else RangeJoinMethod.TwoPhaseJoin - } - - } diff --git a/src/main/scala/org/biodatageeks/sequila/utils/Columns.scala b/src/main/scala/org/biodatageeks/sequila/utils/Columns.scala index 699db2ca..95c00ab7 100644 --- a/src/main/scala/org/biodatageeks/sequila/utils/Columns.scala +++ b/src/main/scala/org/biodatageeks/sequila/utils/Columns.scala @@ -32,6 +32,7 @@ object Columns { final val COUNT_REF="countRef" final val COUNT_NONREF="countNonRef" final val QUALS="quals" + final val LENGTH = "Length" private val sequencedFragmentColumns = ScalaFuncs .classAccessors[SequencedFragment] diff --git a/src/main/scala/org/biodatageeks/sequila/utvf/FeatureCountsTemplate.scala b/src/main/scala/org/biodatageeks/sequila/utvf/FeatureCountsTemplate.scala new file mode 100644 index 00000000..9ca4d837 --- /dev/null +++ b/src/main/scala/org/biodatageeks/sequila/utvf/FeatureCountsTemplate.scala @@ -0,0 +1,32 @@ +package org.apache.spark.sql + +import org.apache.spark.sql.catalyst.analysis.MultiInstanceRelation +import org.apache.spark.sql.catalyst.expressions.Attribute +import org.apache.spark.sql.catalyst.plans.logical.LeafNode +import org.apache.spark.sql.types.{IntegerType, StringType, StructField, StructType} +import org.biodatageeks.sequila.utils.Columns + +case class FeatureCountsTemplate(reads: String, + genes: String, + output: Seq[Attribute]) + extends LeafNode with MultiInstanceRelation with Serializable { + override def newInstance(): FeatureCountsTemplate = copy(output = output.map(_.newInstance())) + + override def toString: String = { + s"FeatureCounts ($reads, $genes)" + } +} + +object FeatureCountsTemplate { + def apply(reads: String, genes: String): FeatureCountsTemplate = { + val output = StructType(Seq( + StructField(s"${Columns.SAMPLE}", StringType, nullable = false), + StructField(s"${Columns.CONTIG}", StringType, nullable = false), + StructField(s"${Columns.START}", IntegerType, nullable = false), + StructField(s"${Columns.END}", IntegerType, nullable = false), + StructField(s"${Columns.STRAND}", StringType, nullable = true), + StructField(s"${Columns.LENGTH}", IntegerType, nullable = false)) + ).toAttributes + new FeatureCountsTemplate(reads, genes, output) + } +} \ No newline at end of file diff --git a/src/main/scala/org/biodatageeks/sequila/utvf/ResolveTableValuedFunctionsSeq.scala b/src/main/scala/org/biodatageeks/sequila/utvf/ResolveTableValuedFunctionsSeq.scala index 062f6ca6..2748fc24 100644 --- a/src/main/scala/org/biodatageeks/sequila/utvf/ResolveTableValuedFunctionsSeq.scala +++ b/src/main/scala/org/biodatageeks/sequila/utvf/ResolveTableValuedFunctionsSeq.scala @@ -19,18 +19,15 @@ package org.apache.spark.sql */ -import java.util.Locale - -import org.apache.spark.sql.ResolveTableValuedFunctionsSeq.tvf import org.apache.spark.sql.catalyst.analysis.{MultiInstanceRelation, TypeCoercion, UnresolvedTableValuedFunction} import org.apache.spark.sql.catalyst.expressions.{Alias, Attribute, Expression} import org.apache.spark.sql.catalyst.plans.logical._ import org.apache.spark.sql.catalyst.rules._ -import org.apache.spark.sql.internal.SQLConf -import org.apache.spark.sql.types.{StructField, _} -import org.biodatageeks.sequila.pileup.conf.QualityConstants +import org.apache.spark.sql.types._ import org.biodatageeks.sequila.utils.Columns +import java.util.Locale + /** * Rule that resolves table-valued function references. @@ -108,13 +105,17 @@ object ResolveTableValuedFunctionsSeq extends Rule[LogicalPlan] { PileupTemplate(table.toString, sampleId.toString, refPath.toString, alts.toString.toBoolean, quals.toString.toBoolean, Some(binSize.toString.toInt)) } ), - "coverage" -> Map( /* coverage(tableName) */ tvf("table" -> StringType, "sampleId" -> StringType, "refPath" -> StringType) { case Seq(table: Any, sampleId: Any, refPath: Any) => PileupTemplate(table.toString, sampleId.toString, refPath.toString, false, false, None) }), + "feature_counts" -> Map( + tvf("reads" -> StringType, "genes" -> StringType) { + case Seq(reads: Any, genes: Any) => + FeatureCountsTemplate(reads.toString, genes.toString) + }), "range" -> Map( /* range(end) */ tvf("end" -> LongType) { case Seq(end: Long) => From 20d0ea715c29c2577a5945a509246bb2aea31e25 Mon Sep 17 00:00:00 2001 From: kkobylin Date: Mon, 27 Jun 2022 16:58:46 +0200 Subject: [PATCH 2/2] GroupBy in FeatureCounts function, add dataset API --- .../sequila/apps/FeatureCountsFunc.scala | 6 ++--- .../IntervalTree/FeatureCountsPlan.scala | 23 +++++++++++++++++-- .../sequila/utvf/FeatureCountsTemplate.scala | 10 +++++--- .../sequila/utvf/SequilaSession.scala | 15 ++++++++++-- 4 files changed, 44 insertions(+), 10 deletions(-) diff --git a/src/main/scala/org/biodatageeks/sequila/apps/FeatureCountsFunc.scala b/src/main/scala/org/biodatageeks/sequila/apps/FeatureCountsFunc.scala index 9c218321..40ca4139 100644 --- a/src/main/scala/org/biodatageeks/sequila/apps/FeatureCountsFunc.scala +++ b/src/main/scala/org/biodatageeks/sequila/apps/FeatureCountsFunc.scala @@ -33,11 +33,11 @@ object FeatureCountsFunc { val ss = SequilaSession(spark) - val query = s"Select fc.*, count(*) AS Counts " + - s" FROM feature_counts('${runConf.readsFile()}', '${runConf.annotations()}') fc " + - s" GROUP BY fc.sample_id, fc.contig, fc.pos_start, fc.pos_end, fc.strand, fc.length" + val query = s"Select fc.*" + + s" FROM feature_counts('${runConf.readsFile()}', '${runConf.annotations()}') fc " ss.sql(query) + .orderBy("sample_id") .coalesce(1) .show() } diff --git a/src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/FeatureCountsPlan.scala b/src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/FeatureCountsPlan.scala index 4f867028..e1148073 100644 --- a/src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/FeatureCountsPlan.scala +++ b/src/main/scala/org/biodatageeks/sequila/rangejoins/methods/IntervalTree/FeatureCountsPlan.scala @@ -3,7 +3,7 @@ package org.biodatageeks.sequila.rangejoins.methods.IntervalTree import org.apache.spark.rdd.RDD import org.apache.spark.sql.SequilaSession.logger import org.apache.spark.sql.catalyst.InternalRow -import org.apache.spark.sql.catalyst.expressions.Attribute +import org.apache.spark.sql.catalyst.expressions.{Attribute, UnsafeProjection} import org.apache.spark.sql.execution.SparkPlan import org.apache.spark.sql.{Row, SparkSession} import org.apache.spark.unsafe.types.UTF8String @@ -83,6 +83,13 @@ case class FeatureCountsPlan(spark: SparkSession, }) }) .flatMap(r => r) + .groupBy(x => x) + .mapValues(_.size) + .map(entry => { + entry._1.setInt(6, entry._2) + toUnsafeRow(entry._1) + }) + v3 } else { val genesRddWithIndex = genesRdd.zipWithIndex() @@ -118,10 +125,21 @@ case class FeatureCountsPlan(spark: SparkSession, val result = v3. join(intGenesRdd) .map(l => l._2._2) + .groupBy(x => x) + .mapValues(_.size) + .map(entry => { + entry._1.setInt(6, entry._2) + toUnsafeRow(entry._1) + }) result } } + private def toUnsafeRow(r: InternalRow): InternalRow = { + val proj = UnsafeProjection.create(schema) + proj.apply(r) + } + private def toInternalRow(r: Row): InternalRow = { InternalRow.fromSeq(Seq( UTF8String.fromString(r.getString(0)), //Sample @@ -129,7 +147,8 @@ case class FeatureCountsPlan(spark: SparkSession, r.getString(2).toInt, //Start r.getString(3).toInt, //End UTF8String.fromString(r.getString(4)), //Strand - r.getString(3).toInt - r.getString(2).toInt //Length + r.getString(3).toInt - r.getString(2).toInt, //Length + 0 //count )) } diff --git a/src/main/scala/org/biodatageeks/sequila/utvf/FeatureCountsTemplate.scala b/src/main/scala/org/biodatageeks/sequila/utvf/FeatureCountsTemplate.scala index 9ca4d837..559c738c 100644 --- a/src/main/scala/org/biodatageeks/sequila/utvf/FeatureCountsTemplate.scala +++ b/src/main/scala/org/biodatageeks/sequila/utvf/FeatureCountsTemplate.scala @@ -24,9 +24,13 @@ object FeatureCountsTemplate { StructField(s"${Columns.CONTIG}", StringType, nullable = false), StructField(s"${Columns.START}", IntegerType, nullable = false), StructField(s"${Columns.END}", IntegerType, nullable = false), - StructField(s"${Columns.STRAND}", StringType, nullable = true), - StructField(s"${Columns.LENGTH}", IntegerType, nullable = false)) + StructField(s"${Columns.STRAND}", StringType, nullable = false), + StructField(s"${Columns.LENGTH}", IntegerType, nullable = false), + StructField(s"${Columns.COUNT_REF}", IntegerType, nullable = false)) ).toAttributes new FeatureCountsTemplate(reads, genes, output) } -} \ No newline at end of file +} + +case class FeatureCounts(sample:String, contig:String, start:Int, end:Int, + strand:String, length:Int, countRef: Int) \ No newline at end of file diff --git a/src/main/scala/org/biodatageeks/sequila/utvf/SequilaSession.scala b/src/main/scala/org/biodatageeks/sequila/utvf/SequilaSession.scala index 931cb6c7..afb9879d 100644 --- a/src/main/scala/org/biodatageeks/sequila/utvf/SequilaSession.scala +++ b/src/main/scala/org/biodatageeks/sequila/utvf/SequilaSession.scala @@ -7,9 +7,8 @@ import org.apache.spark.sql.catalyst.analysis.{Analyzer, SeQuiLaAnalyzer} import org.apache.spark.sql.catalyst.plans.logical.LogicalPlan import org.apache.spark.sql.execution.QueryExecution import org.apache.spark.sql.execution.datasources.SequilaDataSourceStrategy -import org.apache.spark.sql.functions.{lit, typedLit} +import org.apache.spark.sql.functions.typedLit import org.apache.spark.sql.internal.SessionState -import org.apache.spark.sql.types.{ArrayType, ByteType, MapType, ShortType} import org.biodatageeks.sequila.pileup.PileupStrategy import org.biodatageeks.sequila.rangejoins.IntervalTree.IntervalTreeJoinStrategyOptim import org.biodatageeks.sequila.utils.{InternalParams, UDFRegister} @@ -91,6 +90,18 @@ case class SequilaSession(sparkSession: SparkSession) extends SparkSession(spark new Dataset(sparkSession, PileupTemplate(path, refPath, false, false), Encoders.kryo[Row]).as[Coverage] } + /** + * Calculate genes count number in reads + * + * @param reads BAM file + * @param genes BED file + * @return genes count as Dataset[FeatureCounts] + */ + def featureCounts(reads: String, genes: String) : Dataset[FeatureCounts] ={ + + new Dataset(sparkSession, FeatureCountsTemplate(reads, genes), Encoders.kryo[Row]).as[FeatureCounts] + } + /** * Calculate pileup in block/base format * +------+---------+-------+------------------+--------+--------+-----------+---------+--------------------+