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[e] No alignments found to concatenate #104

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jwkkang opened this issue Feb 24, 2023 · 1 comment
Open

[e] No alignments found to concatenate #104

jwkkang opened this issue Feb 24, 2023 · 1 comment
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@jwkkang
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jwkkang commented Feb 24, 2023

Hello,

I have run my code as below.

phylophlan -i ./spades_output/final_contigs_test --genome_extension .fasta \
-d phylophlan --databases_folder ./phylophlan_databases \
--diversity low \
--fast \
--verbose \
-f ./phylophlan_configs/supermatrix_aa.cfg \
-o ./phylophlan_output/fasta_contigs_phylophlan_test_slurm

But I got an error message. [e] No alignments found to concatenate.
Could you please let me know what is the issue here?

I have attached the log file.

Thank you,

slurm-894215.txt

@fasnicar fasnicar self-assigned this May 18, 2023
@fasnicar
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Hello, from the log it seems you only provided 3 input files, while the minimum number for building a phylogenetic tree is 4. Hence all markers are discarded because only 3/4 genomes are found and hence there is nothing to concatenate.

Many thanks,
Francesco

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