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MuPeXI finds peptide in a transcript which is labeled as "Nonsense mediated decay" #18

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gilhornung opened this issue Jun 5, 2018 · 0 comments

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@gilhornung
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Dear Anne-Mette,

I came across an issue in MuPeXI.
I have a mutation in 3:48605804 (GRCH38, see below for full vcf line), which occurs in the gene UQCRC1.
MuPeXI finds peptides in the ENST00000415995 transcript, which is actually a "Nonsense mediated decay" transcript, based on its ensembl annotation. Obviously, it should not be considered as a source for peptides.

All the best,

Gil

vcf line:

3       48605804        .       C       T       122.87  PASS    .       GT:AD:AF:ALT_F1R2:ALT_F2R1:FOXOG:QSS:REF_F1R2:REF_F2R1  0/1:179,62:0.257:.:.:.:.:.:.     0/0:121,0:0:.:.:.:.:.:.

one of the peptides in the mupexi file:

HLA-C06:02 .G....... 6.5456 0.025697 9511.3 3.6350 0.153380 MRQATFWSI 0.2283 0.408440 223.3 0.0797 0.500108 ENSG00000010256 ENST00000415995 G/R 0.257 1 2 3 48605804 43 M UQCRC1 No No 0.411875 9.998579e-01 1.346961e-10 0.4714048 12
@gilhornung gilhornung changed the title MuPeXI find peptide in a transcript which is labeled as "Nonsense mediated decay" MuPeXI finds peptide in a transcript which is labeled as "Nonsense mediated decay" Jun 5, 2018
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