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I came across an issue in MuPeXI.
I have a mutation in 3:48605804 (GRCH38, see below for full vcf line), which occurs in the gene UQCRC1.
MuPeXI finds peptides in the ENST00000415995 transcript, which is actually a "Nonsense mediated decay" transcript, based on its ensembl annotation. Obviously, it should not be considered as a source for peptides.
All the best,
Gil
vcf line:
3 48605804 . C T 122.87 PASS . GT:AD:AF:ALT_F1R2:ALT_F2R1:FOXOG:QSS:REF_F1R2:REF_F2R1 0/1:179,62:0.257:.:.:.:.:.:. 0/0:121,0:0:.:.:.:.:.:.
one of the peptides in the mupexi file:
HLA-C06:02
.G.......
6.5456
0.025697
9511.3
3.6350
0.153380
MRQATFWSI
0.2283
0.408440
223.3
0.0797
0.500108
ENSG00000010256
ENST00000415995
G/R
0.257
1
2
3
48605804
43
M
UQCRC1
No
No
0.411875
9.998579e-01
1.346961e-10
0.4714048
12
The text was updated successfully, but these errors were encountered:
gilhornung
changed the title
MuPeXI find peptide in a transcript which is labeled as "Nonsense mediated decay"
MuPeXI finds peptide in a transcript which is labeled as "Nonsense mediated decay"
Jun 5, 2018
Dear Anne-Mette,
I came across an issue in MuPeXI.
I have a mutation in 3:48605804 (GRCH38, see below for full vcf line), which occurs in the gene UQCRC1.
MuPeXI finds peptides in the ENST00000415995 transcript, which is actually a "Nonsense mediated decay" transcript, based on its ensembl annotation. Obviously, it should not be considered as a source for peptides.
All the best,
Gil
vcf line:
one of the peptides in the mupexi file:
The text was updated successfully, but these errors were encountered: