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Multi Mapping Loci Meaning #2236

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mattg478 opened this issue Nov 6, 2024 · 0 comments
Open

Multi Mapping Loci Meaning #2236

mattg478 opened this issue Nov 6, 2024 · 0 comments

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@mattg478
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mattg478 commented Nov 6, 2024

Hi Thanks for this super useful software!

I have some Parse Bioscience snRNA data that I have been trying to map.
I get lots of multimapping reads (>70%) and have been trying to work out what the issue is.

Would you be able to clarify what exactly counts as a loci for multi mapping?
Do reads mapping to two different exons across a splice site count as multiple loci?
Do reads mapping across an exon/intron boundary count multiple loci?
If there are two genomic features overlapping (eg a non-coding gene in the intron of another gene) would a read mapping here count multiple loci?

Thank you,
Matt

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