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TeamVITVelloreParts.html
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{{VIT_Vellore}}
{{VIT_Vellore/CSS}}
{{VIT_Vellore/JS}}
{{VIT_Vellore/JSold}}
{{VIT_Vellore/Popper}}
{{VIT_Vellore/JS2}}
<html lang="en">
<head>
<meta charset="UTF-8">
<meta name="viewport" content="width=device-width, initial-scale=1">
<link rel='shortcut icon' href='https://2019.igem.org/wiki/images/9/9d/T--VIT_Vellore--Projectlogo.png' type='image/x-icon'>
<link rel='icon' href='https://2019.igem.org/wiki/images/9/9d/T--VIT_Vellore--Projectlogo.png' type='image/x-icon'>
<link href="https://fonts.googleapis.com/css?family=Comfortaa&display=swap" rel="stylesheet">
<link href="https://fonts.googleapis.com/css?family=Amiri&display=swap" rel="stylesheet">
<link href="https://fonts.googleapis.com/css?family=Cardo&display=swap" rel="stylesheet">
<link href="https://fonts.googleapis.com/css?family=PT+Serif&display=swap" rel="stylesheet">
<link href="https://fonts.googleapis.com/css?family=Crimson+Pro&display=swap" rel="stylesheet">
<link href="https://fonts.googleapis.com/css?family=Quicksand|Raleway&display=swap" rel="stylesheet">
<title>Team VIT Vellore/Description</title>
<style>
::selection{background-color: maroon; color: white;}
#bodyContent { background: transparent;}
#content { background: transparent;}
#globalWrapper {
padding-bottom: 0px;
}
.sidenav {
height: 100%;
width: 0;
position: fixed;
z-index: 100;
top: 0;
left: 0;
background-color: #2b2b2b;
overflow-x: hidden;
transition: 0.5s;
box-shadow: 8px 0 10px -6px black;
}
.sidenav a {
padding: 2px 8px 2px 22px;
text-decoration: none;
font-size: 25px;
color: white;
display: block;
transition: 0.3s;
}
.sidenav a:hover {
color: #f1f1f1;
}
.dropdown-menu {
border-radius: 0;
}
li.dropdown .dropdown-menu {
padding: 0;
margin: 0;
}
li.dropdown .dropdown-menu .dropdown-item {
color: #566573;
font-size: 11px;
font-weight: bold;
padding-top: 15px;
padding-bottom: 15px;
transition: 0.25s;
text-decoration: none;
}
li.dropdown .dropdown-menu .dropdown-item:hover {
color: rgb(3,251,32);
padding-left: 28px;
}
.nav1_sidebar .dropdown-item, .nav2_sidebar .dropdown-item, .nav3_sidebar .dropdown-item, .nav4_sidebar .dropdown-item, .nav5_sidebar .dropdown-item, .nav6_sidebar .dropdown-item {
color: white;
padding-top: 0;
padding-bottom: 0;
transition: 0.25s;
margin-left: 3px;
text-decoration: none;
background: transparent;
}
.nav1_sidebar .dropdown-item:hover, .nav2_sidebar .dropdown-item:hover, .nav3_sidebar .dropdown-item:hover, .nav4_sidebar .dropdown-item:hover, .nav5_sidebar .dropdown-item:hover, .nav6_sidebar .dropdown-item:hover {
color: rgb(3,251,32);
margin-left: 28px;
background: transparent;
}
.mainbtn {
display: none;
background: transparent;
border: none;
font-size: 28px;
cursor: pointer;
-webkit-tap-highlight-color: transparent;
color: black;
}
.mainbtn1 {
font-size: 14px;
font-weight: bold;
border-radius: 0;
border: 2px solid silver;
transition: 0.25s;
background: transparent;
margin-top: 20px;
margin-bottom: 20px;
color: silver;
}
.mainbtn1:hover {
border: 2px solid crimson;
background-color: crimson;
color: white;
}
.main2 {
margin-left: 70px;
margin-top: 30px;
}
.main3 {
margin-top: 23vh;
position: relative;
}
.main4 {
background: rgba(210, 217, 221, 0.95);
top: 55px;
margin-left: -50px;
}
.main5e table {color: white; margin-top: 50px; border: 1.5px solid white;}
.main5e table thead th{border: none; letter-spacing: 1.2px;}
.main5e table td{border: none;}
.main5e td{
font-family: 'Quicksand', sans-serif;
font-size: 15px;
}
.main5e h2{
font-family: 'Quicksand', sans-serif;
}
.main5f th {
font-weight: bold;
font-size: 32px;
font-family: 'Raleway', sans-serif;
}
.main5f {font-family: 'Raleway', sans-serif;}
.main5f p{
font-size: 17px;
}
.overlay {
width: 100%;
position: relative;
top: 90px;
margin-top: 0px;
padding-top: 0;
z-index: 40;
background-color: #383838;
transition: 1.5s;
}
.overlay-content {
position: relative;
top: 25%;
width: 100%;
text-align: center;
margin-top: 30px;
}
.overlay a:focus {
color: black;
}
.fade {
margin: 0;
padding: 0;
transition: 1s;
-webkit-animation-name: fade;
-webkit-animation-duration: 5.5s;
animation-name: fade;
animation-duration: 5.5s;
}
.mainbtn div {
width: 25px;
height: 3px;
background-color: white;
color: white;
margin: 6px 0;
}
#logo1 {transition: 0.5s;}
.main5c:nth-child(odd) h2{text-align: right;}
.main5c:nth-child(odd) .main5d{text-align: right;}
.main5c{padding: 0; margin: 40px 0 40px 0; background-color: #107896; color: white;}
.main5c hr{color: white; background-color: white;}
.main5 img{background: black; border-radius: 0; height: 375px; width: 100%; border: 8px solid white; box-shadow: 0 4px 6px 0 rgba(0, 0, 0, 0.1), 0 6px 12px 0 rgba(0, 0, 0, 0.3); z-index: 70;}
.main3a {text-align: right; font-family: 'Raleway', sans-serif;}
.main5a{text-align: center;}
.main5b{position: relative; width: 100%; height: 84vh; margin: 0; padding: 0; background: linear-gradient(rgba(255,255,255,0.82),rgba(255,255,255,0.82)), url(https://2019.igem.org/wiki/images/7/75/T--VIT_Vellore--BackParts.jpeg); top: 0; background-repeat: repeat; background-position: center; cursor: pointer; transition: 0.5s; background-attachment: fixed;}
.main5b:hover {background: linear-gradient(rgba(255,255,255,0.82),rgba(255,255,255,0.82)), url(https://2019.igem.org/wiki/images/7/75/T--VIT_Vellore--BackParts.jpeg); background-repeat: repeat; background-position: right; background-attachment: fixed;}
.main5b:hover #logo1 {color: rgb(0,210,51); text-shadow: white 3px 2px 0;}
.mainhead1 {font-weight: bold; color: white; font-size: 70px; padding-right: 30px; padding-top: 65px;}
.mainimg{width:100%; margin: 0; padding: 0; transition: 1s;height: 380px; background-color: black;}
.mainimg1{width: 120px; height: 90px; padding: 10px 0 10px 0;}
.mainimg2{display: none;}
.mainimg4{width: 70px; height: auto; padding: 0 0 10px 0;}
.mainpara{color: white; font-weight: bold; font-size: 14px; padding-right: 30px;}
.mainpara1{color: white; font-weight: bold; padding-right: 30px; font-size: 14px;}
@-webkit-keyframes fade {
from {opacity: .8}
to {opacity: 1}
}
@keyframes fade {
from {opacity: .8}
to {opacity: 1}
}
#logo {
font-family: 'Raleway', sans-serif;
color: black;
text-decoration: none;
text-transform: uppercase;
font-size: 5vw;
font-weight: bold;
letter-spacing: -3px;
line-height: 1;
text-shadow: #CCCCCC 3px 2px 0;
position: relative;
transition: 0.5s;
}
@media screen and (max-height: 991px) {
.sidenav a {font-size: 18px;}
}
@media screen and (max-width: 1200px) {
.mainhead1 {font-size: 62px;}
}
@media screen and (max-width: 1024px) {
.mainimg {height: 320px;}
}
@media screen and (max-width: 991px) {
.mainbtn {display: block;}
.mainimg3{display: none;}
.mainimg2{display: block; position: absolute; left: 210px; padding-bottom: 13px;}
.main3 {margin-top: 100px;}
.main3a{text-align: left;}
.mainimg{height: 285px;}
.main5c{margin: 0;}
}
@media screen and (max-width: 992px) {
.main2 {margin-top: 8px;margin-left: 30px;}
.mainhead1 {padding-top: 24px; font-size: 58px;}
.main4 {top: 0;margin-left: 0;}
.mainbtn1{margin-top: 2px;}
.item-header {left: 14px;}
.main5c:nth-child(odd) h2{text-align: left;}
.main5c:nth-child(odd) .main5d{text-align: left;}
.overlay{top: 60px;}
#logo {font-size: 8vw;}
}
@media screen and (max-width: 425px) {
.mainhead1 {font-size: 50px;}
.main3 {margin-top: 120px;}
.mainimg{height: 283px;}
.mainimg2{left: 180px;}
.mainimg4{width: 70px;}
.mainpara, .mainpara1 {font-size: 10px;}
.mainbtn1 {font-size: 10px;}
}
@media screen and (max-width: 320px) {
.mainimg2{left: 103px;}
.mainimg4{width: 70px;}
.mainimg1{width: 80px;}
}
.focus-in-contract {
-webkit-animation: focus-in-contract 0.7s cubic-bezier(0.250, 0.460, 0.450, 0.940) 1s both;
animation: focus-in-contract 0.7s cubic-bezier(0.250, 0.460, 0.450, 0.940) 1s both;
}
@-webkit-keyframes focus-in-contract {
0% {
letter-spacing: 1em;
-webkit-filter: blur(12px);
filter: blur(12px);
opacity: 0;
}
100% {
-webkit-filter: blur(0px);
filter: blur(0px);
opacity: 1;
}
}
@keyframes focus-in-contract {
0% {
letter-spacing: 1em;
-webkit-filter: blur(12px);
filter: blur(12px);
opacity: 0;
}
100% {
-webkit-filter: blur(0px);
filter: blur(0px);
opacity: 1;
}
}
.bounce-in-left {
-webkit-animation: bounce-in-left 1.1s 1s both;
animation: bounce-in-left 1.1s 1s both;
}
@-webkit-keyframes bounce-in-left {
0% {
-webkit-transform: translateX(-600px);
transform: translateX(-600px);
-webkit-animation-timing-function: ease-in;
animation-timing-function: ease-in;
opacity: 0;
}
38% {
-webkit-transform: translateX(0);
transform: translateX(0);
-webkit-animation-timing-function: ease-out;
animation-timing-function: ease-out;
opacity: 1;
}
55% {
-webkit-transform: translateX(-68px);
transform: translateX(-68px);
-webkit-animation-timing-function: ease-in;
animation-timing-function: ease-in;
}
72% {
-webkit-transform: translateX(0);
transform: translateX(0);
-webkit-animation-timing-function: ease-out;
animation-timing-function: ease-out;
}
81% {
-webkit-transform: translateX(-28px);
transform: translateX(-28px);
-webkit-animation-timing-function: ease-in;
animation-timing-function: ease-in;
}
90% {
-webkit-transform: translateX(0);
transform: translateX(0);
-webkit-animation-timing-function: ease-out;
animation-timing-function: ease-out;
}
95% {
-webkit-transform: translateX(-8px);
transform: translateX(-8px);
-webkit-animation-timing-function: ease-in;
animation-timing-function: ease-in;
}
100% {
-webkit-transform: translateX(0);
transform: translateX(0);
-webkit-animation-timing-function: ease-out;
animation-timing-function: ease-out;
}
}
@keyframes bounce-in-left {
0% {
-webkit-transform: translateX(-600px);
transform: translateX(-600px);
-webkit-animation-timing-function: ease-in;
animation-timing-function: ease-in;
opacity: 0;
}
38% {
-webkit-transform: translateX(0);
transform: translateX(0);
-webkit-animation-timing-function: ease-out;
animation-timing-function: ease-out;
opacity: 1;
}
55% {
-webkit-transform: translateX(-68px);
transform: translateX(-68px);
-webkit-animation-timing-function: ease-in;
animation-timing-function: ease-in;
}
72% {
-webkit-transform: translateX(0);
transform: translateX(0);
-webkit-animation-timing-function: ease-out;
animation-timing-function: ease-out;
}
81% {
-webkit-transform: translateX(-28px);
transform: translateX(-28px);
-webkit-animation-timing-function: ease-in;
animation-timing-function: ease-in;
}
90% {
-webkit-transform: translateX(0);
transform: translateX(0);
-webkit-animation-timing-function: ease-out;
animation-timing-function: ease-out;
}
95% {
-webkit-transform: translateX(-8px);
transform: translateX(-8px);
-webkit-animation-timing-function: ease-in;
animation-timing-function: ease-in;
}
100% {
-webkit-transform: translateX(0);
transform: translateX(0);
-webkit-animation-timing-function: ease-out;
animation-timing-function: ease-out;
}
}
</style>
</head>
<body style="font-size:80%">
<div class="container row" style="width: 100%;">
<nav class="navbar navbar-expand-lg " style="position: fixed; top: 0; padding-top: 20px; transition: 0.3s; width: 100%; z-index: 70; padding-bottom: 0px; background: #2b2b2b; box-shadow: 0px 5px 10px #888888;">
<a class="navbar-brand" href="https://2019.igem.org/Team:VIT_Vellore" style=" text-decoration: none;"><img src="https://2019.igem.org/wiki/images/d/da/T--VIT_Vellore--Projectlogo1.png" class="mainimg4 img-fluid" /></a>
<div class="collapse navbar-collapse justify-content-end" id="navbarNavDropdown" style=" padding-right: 30px;">
<ul class="navbar-nav" style="font-family: 'Quicksand', sans-serif; margin-top: -20px; position: relative;">
<li class="nav-item dropdown nav1">
<a class="nav-link navh1 px-4" href="#" id="navbarDropdownMenuLink" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false" style=" color: white; padding-bottom: 9px; padding-top: 39px; transition: 0.25s; text-decoration: none; font-size: 15px; letter-spacing: 1.3px; text-align: center; font-weight: bold;">Project</a>
<div class="dropdown-menu d1" aria-labelledby="navbarDropdownMenuLink" style="font-size: 13px;">
<a class="dropdown-item" style="padding-top: 10px;" href="https://2019.igem.org/Team:VIT_Vellore/Description">Description</a>
<a class="dropdown-item" href="https://2019.igem.org/Team:VIT_Vellore/Parts">Parts</a>
<a class="dropdown-item" href="https://2019.igem.org/Team:VIT_Vellore/Design">Design</a>
<a class="dropdown-item" href="https://2019.igem.org/Team:VIT_Vellore/Model">Model</a>
<a class="dropdown-item" href="https://2019.igem.org/Team:VIT_Vellore/Experiments">Experiments</a>
<a class="dropdown-item pb-2" href="https://2019.igem.org/Team:VIT_Vellore/Results">Results</a>
</div>
</li>
<li class="nav-item dropdown nav2">
<a class="nav-link navh2 px-4" href="https://2019.igem.org/Team:VIT_Vellore/Safety" id="navbarDropdownMenuLink" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false" style=" color: white; padding-bottom: 9px; padding-top: 39px; transition: 0.25s; text-decoration: none; font-size: 15px; letter-spacing: 1.3px; font-weight: bold;">Safety</a>
</li>
<li class="nav-item dropdown nav4">
<a class="nav-link navh4 px-4" href="https://2019.igem.org/Team:VIT_Vellore/Notebook" id="navbarDropdownMenuLink" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false" style=" color: white; padding-bottom: 9px; padding-top: 39px; transition: 0.25s; text-decoration: none; font-size: 15px; letter-spacing: 1.3px; font-weight: bold;">Notebook</a>
</li>
<li class="nav-item dropdown nav3">
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<h2 class="display-4" style="font-weight: bold; padding: 70px 20px 70px 20px; font-size: 35px;"><span style="color: rgb(0,210,51);">Regulatory</span> and <span style="color: rgb(0,210,51);">coding</span> parts used by us</h2>
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<th>Part</th>
<th>Type</th>
<th>Source</th>
<th>Description</th>
<th>Function</th>
<th>Length</th>
</tr>
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<td>BBA_K3053001</td>
<td>Regulatory</td>
<td style="font-style: italic;">Escherichia coli</td>
<td>Ribosome Binding Site (RBS)</td>
<td>The antisense gene is flanked by an RBS sequence on the 5' end and a complementary RBS sequence on the 3' end. The RBS forms a duplex by binding to its complementary RBS sequence in the absence of antibiotic resistance gene & thus, inactivates the expression of the Cro gene. When the antibiotic resistant gene is present, the RBS binds to the complement RBS in the mRNA of the resistant bacterium & enables the expression of the Cro gene.</td>
<td>78bp</td>
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<td>BBA_0015</td>
<td>Regulatory</td>
<td style="font-style: italic;">Escherichia coli</td>
<td>Double Terminator</td>
<td>The double terminator terminates the transcription of the antisense construct mRNA.</td>
<td>129bp</td>
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<td>BBA_K3053000</td>
<td>Regulatory</td>
<td style="font-style: italic;">Lambda Phase</td>
<td>Constitutive Promoter</td>
<td>The constitutive promoter activates the transcription of all the genes in the construct.</td>
<td>49bp</td>
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<td>BBA_K3053002</td>
<td>Coding</td>
<td style="font-style: italic;">Lambda Phage</td>
<td>Cro Gene</td>
<td>Cro protein blocks promoter of cI protein. Expression of cI protein is responsible for maintaining the phage lysogenic cycle. Therefore, expression of Cro switches the phage from its lysogenic to lytic cycle.</td>
<td>201bp</td>
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<td>BBA_K3053069</td>
<td>Regulatory</td>
<td>None</td>
<td>Reverse Complement</td>
<td>Forms a dsRNA loop in absence of antibiotic resistance gene and inhibits expression of Cro gene.</td>
<td>133bp</td>
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