From 8ba4a8c13e4b6675bf831f20b7ad67a370b1ca34 Mon Sep 17 00:00:00 2001 From: mikejiang Date: Tue, 3 Apr 2018 11:53:01 -0700 Subject: [PATCH] update README --- README.Rmd | 21 ++++++++ README.md | 49 +++++++++++++++--- .../figure-html/unnamed-chunk-4-1.png | Bin 8761 -> 14938 bytes .../figure-html/unnamed-chunk-4-2.png | Bin 9078 -> 16817 bytes .../figure-html/unnamed-chunk-4-3.png | Bin 8424 -> 15069 bytes .../figure-html/unnamed-chunk-4-4.png | Bin 9217 -> 18988 bytes .../figure-html/unnamed-chunk-4-5.png | Bin 8899 -> 16514 bytes .../figure-html/unnamed-chunk-4-6.png | Bin 9408 -> 16817 bytes .../figure-html/unnamed-chunk-4-7.png | Bin 7465 -> 13509 bytes .../figure-html/unnamed-chunk-5-1.png | Bin 1723 -> 7212 bytes .../figure-html/unnamed-chunk-5-2.png | Bin 0 -> 8675 bytes .../figure-html/unnamed-chunk-6-1.png | Bin 8128 -> 1550 bytes .../figure-html/unnamed-chunk-7-1.png | Bin 11401 -> 7302 bytes .../figure-html/unnamed-chunk-8-1.png | Bin 11808 -> 27806 bytes .../figure-html/unnamed-chunk-9-1.png | Bin 0 -> 29440 bytes 15 files changed, 64 insertions(+), 6 deletions(-) create mode 100644 README_files/figure-html/unnamed-chunk-5-2.png create mode 100644 README_files/figure-html/unnamed-chunk-9-1.png diff --git a/README.Rmd b/README.Rmd index 6886246..e67705d 100644 --- a/README.Rmd +++ b/README.Rmd @@ -72,6 +72,27 @@ p + geom_overlay("IgD-CD27-", alpha = 0.5, size = 0.1, color = "purple") fs <- getData(gs, "CD20") #extract the gated data as a flowSet autoplot(fs, "CD20", "CD19") #plot 2D ``` +```{r} +gs1 <- gs[1] +gs2 <- gs[2] +#construct the ggcyto object for gs1 +p <- ggcyto(gs1, aes(cd24, cd38)) + geom_hex(bins = 128) +p <- p + geom_gate("Transitional") #add gate +#customize the stats layer +p <- p + geom_stats(type = "count", size = 6, color = "red", fill = "green", adjust = 0.7) +#customize the layer +p <- p + labs_cyto("channel") +#customize the axis limits +p <- p + ggcyto_par_set(limits = "instrument") +#add another population as the overlay dots +p <- p + geom_overlay("IgD-CD27-", col = "black", size = 1.2, alpha = 0.4) +#hide the legend +p <- p + guides(fill=FALSE) +p + +#replace the data with gs2 and see the same visual effect +p %+% gs2 +``` ```{r fig.height = 1} autoplot(fs, "CD20") #1d density diff --git a/README.md b/README.md index d6de366..549df18 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,14 @@ -# ggcyto : Visualize `Cytometry` data with `ggplot`' - +--- +title: 'ggcyto : Visualize `Cytometry` data with `ggplot`' +output: + html_document: + fig_height: 1 + fig_width: 1 + keep_md: yes +vignette: > + %\VignetteEngine{knitr::rmarkdown} + %\VignetteIndexEntry{Feature summary of ggcyto} +--- `ggcyto` is a cytometry data visualization tool built around ggplot and the grammar of graphics paradigm. The software extends the popular ggplot2 framework, already familiar to many data scientists, enabling it to recog-nize the core Bioconductor flow cytometry data structures for gated and annotated cytometry data. It simplifies visualization and plotting of flow data for publication quality graphics. @@ -93,12 +102,40 @@ autoplot(fs, "CD20", "CD19") #plot 2D ![](README_files/figure-html/unnamed-chunk-4-7.png) +```r +gs1 <- gs[1] +gs2 <- gs[2] +#construct the ggcyto object for gs1 +p <- ggcyto(gs1, aes(cd24, cd38)) + geom_hex(bins = 128) +p <- p + geom_gate("Transitional") #add gate +#customize the stats layer +p <- p + geom_stats(type = "count", size = 6, color = "red", fill = "green", adjust = 0.7) +#customize the layer +p <- p + labs_cyto("channel") +#customize the axis limits +p <- p + ggcyto_par_set(limits = "instrument") +#add another population as the overlay dots +p <- p + geom_overlay("IgD-CD27-", col = "black", size = 1.2, alpha = 0.4) +#hide the legend +p <- p + guides(fill=FALSE) +p +``` + +![](README_files/figure-html/unnamed-chunk-5-1.png) + +```r +#replace the data with gs2 and see the same visual effect +p %+% gs2 +``` + +![](README_files/figure-html/unnamed-chunk-5-2.png) + ```r autoplot(fs, "CD20") #1d density ``` -![](README_files/figure-html/unnamed-chunk-5-1.png) +![](README_files/figure-html/unnamed-chunk-6-1.png) ```r #extract one sample as a flowFrame @@ -107,7 +144,7 @@ fr <- fs[[1]] autoplot(fr) ``` -![](README_files/figure-html/unnamed-chunk-6-1.png) +![](README_files/figure-html/unnamed-chunk-7-1.png) @@ -119,7 +156,7 @@ p <- autoplot(gh, nodes, bins = 64) p ``` -![](README_files/figure-html/unnamed-chunk-7-1.png) +![](README_files/figure-html/unnamed-chunk-8-1.png) ```r @@ -128,7 +165,7 @@ gt <- ggcyto_arrange(p, nrow = 1) plot(gt) ``` -![](README_files/figure-html/unnamed-chunk-8-1.png) +![](README_files/figure-html/unnamed-chunk-9-1.png) diff --git a/README_files/figure-html/unnamed-chunk-4-1.png b/README_files/figure-html/unnamed-chunk-4-1.png index 840ca0a1519fce472271bd42cc3ed9e8bbcb63de..2241eff6f30a09908aa2af6eb40929c08885ce38 100755 GIT binary patch literal 14938 zcma)j19T+a_ie|R*tTsu6FZq;!ijCWV@^0pCbm5jdt%$RZRhoT-|ykS9@eYXy;5DN zuCBWG+=G4gj!;&VLWIYI2LJ$wGSZ(_0RXT~(3=Dn9CTzypW6-e2hKtIt1|$A)bsZZ zHeg?30sxQzWIl_2@yIyK^vuvzPkr#O*VMdQpIhR4fd;n%k2e*$M~TZFA}K*J(#7FS z!|u+D-f83G<2$A~`RwR3#R`cD?UI8j28*LC5&$p^ixU}~mT}j-Seu*E8}a?%X&e`P z(3m@0sn626^AUd9bv_pa9df;J&Jwy4IN*&HSHVDm6h*n-VsM9n4I~L4jSHlL4UEH3 zodg|C5Q9%6ffZAg7Da~?m4>Ia2qb}(aZG82NZfJY0nkKt!&m6e6PXr4uIGAQpkQ{_1_Q05;;6ybxtg8=&WYeH=1W^@5_iPOowZa9`kZ}DJMK_hosCS$@b>NX@ zNzaEP56TjEHHyXMkfAO&&!jF={WLEu|avb^$ePIoi zNMv?eFysq|Zy1YP2Nq;fOswcd^?{%I@Mk;KhWgW-c+`a9^o3C^wZ`($R@$oF4}2HgkUU$mj5IRUYh-!9iyo&ikwF*Qg z+_C2e%rPm!Q680j&w`Fe#2?K$lWVZVi)Y6*pD&0Amz6li|G8h3agTwm)|8>!483cqpc2`fXZZxQ*dO9)=-I#A|0!vuf7y z8T|XA^W%56LZz>#lA<;5>0QI_q=h!_yP2ZtETso)ucJQ%?warZI217mb5Z*uLD?)l zpI!SrN<2$8tDuO|LHBvzeU6~*bA#{w5!WlGUAp_>aRh}**^|tEXa$7!SIHfaTLf=i z&Y?&9g@c9Z)fRk~gIgCp`C3&nxkGshzLWzONJ0@}_J1{DQYZx!NK)E@f6v3<5Jd?t z_>bLtinqI8tm~aKp?KE198DW-1O^v?(*$HJVyFGDi{Srtg_NbQTNS^@muro~wh-vU z4{ii3-_CKXQIRV-($ARG+B1_bOsA<{q>%`dzq7$d^wFDQq1{v~vx<>1x~We>r|wfX zSD0Wd5h!YRdUGLc>kC+NaR?oy)UW?gplyxQYC}W&6fvY(f7sadM-7^E2pu=Lx4;;agd)LM6UsJ*CY;~xlm*q| zL=&qzjCXWA65bn0gG!?zOFT(l+R0D$8dgSfOdnB9)s0SB)cgh2T;J5sH0y0^FWPol z!e{2iG9{~6jg7RHMw;!@(2ssGO1KDwFKK}dO!Aa3JwlW>Mp!nnhZ=EHN@olsu7aVf)eZPJRs*=M&P+1YOcdT+w(k6AVfBi zFc-*Sw`W3w@oT+smE{SGZ4j<9sKMzMq?3`sV6W)^G+_ySbSYP-H+7x9p7is!99=ZTO1jU)* zMMNN*L4;k<+`G2>dej-DpAvl$K3<=TV*ur~^ zz9vFLHEBiI?hNpi9CoD2`+GM#agHCN`+a4>yTK$C%0EOf9bHIYy5!9JA~DF=WKXMMJess&wNaB9EH=-2+ zukN&>FGSCvb4x)_Y*Y$V!COq@2ou-I=%0{({`yX^>X12OT(aOW+?WuSzR z>80Y$-WDu3oVM_gn{1Q7=Hby4wWIShEs9dNXDb5-0Z71=?Nm@dF2BwYatA8QM@YSiPG?*EwY^gn0gkt5BH zJ@>zE10-@(P|`ANR%t{%aOc4zkV~!Om<`TKPX2a(tDc1xeA@_6uM15pp5ZiM4d9vQXH>oyd5T z%5wHHMN`qS&y17Tk}xz$$qy$bdggk}A-_U{nxGd60e9vJep^V2$bjp7EatwSfM|i4*;^ zG@U?(OOj;Jb^a$TFNpGJs19G|xPU!*cL93^+1TMFyw)nTARH^v9yQU(qvq7r8O_hu zWwphU5;%rjp%B}~;(;W$Q$2E6#{uiCJ`~ZKwjdhiX>eMV2^Ux*pqn*3EB6)C#fyze zbqrE;Fk(b6b(yvY*@tzEtXR6y-rk;Bb>uUpsFT>w9VhHf11t&Si=$pdq2syt7q7U&w*^gTG* zO0Dl{X2dGJrlK33Pd}id)pNDL+k8~{=wXWU{)>a{dOcB zHIbs`MV#?#v=?L+(G5N0!rG{JqCzE2IF5(bP8~0$W&++^zW@5 zA8l7_^kh8IwnbyTIr_^#54rUxY>Oy(Wk9(%;GliITA>sipRyFyzYDP!Ov2D52HjL9 zT!`izA>O|FiH3dYrq(Z`!HA!gvW~ojaO!{rB6Io27s7|u44J!B1K+is&gPRuBl5f& zOmRg9rI11`uOx-M# z_m5HgKl|Ng;KCE|N1AAJ5Fl?u6M06#5akwnXX@7G zyx%^`GlnXK{hb4xg8FlAs#}T~7lQw}PgFMi+cheEN|GFZkI`^>>HP#tO9gTpCcVbx z^>`*fyM9k1a$zxH%e7jkI)8>V_|K9D@XP4_rJ<~l?*Oq2WN@4;EWqULI!QU6T{%Qo zMT>wy0B85;46YhMZI(ReOFXIPw3am@V55r%SeuOJ#&+Zc9zN?0_Ndly-LwGJ#g7HE zDNg|g85JJ1BW8vVZ0xX&s#&iLW8QH1|WEpFM#oQ}|oKP_?V-Mdm}S{N*oEDIFI~*@SC4 zf8HU|Jxl{4h;LdRgMd&xf$dy-d9{u)!_04xP`s>%7Cox)s3%TFP60GxwO-X)NiN5u zvFUz3_h0atPx}hxJvkruz-bOcvt^5O7*ly@K8KLNraEFdGsoO2TRDzpSiIMJO!L)! 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