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I have exactly the same problem and I am using R 4.3.2 on windows.
But when I turm back to older R versions like 4.2.0, I can analyze multiple fcs files without any problems. Maybe this is caused by the changes in some dependent R packages in newer R versions.
I keep getting the above error message whenever I load more than 1 fcs file...
I have 12 fcs files.
Using R. 4.3.1 on Mac and Biocunductor 3.17.
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