diff --git a/ddtrace/profiling/collector/_lock.py b/ddtrace/profiling/collector/_lock.py
index ec62c5c0ee..ec0c438b2b 100644
--- a/ddtrace/profiling/collector/_lock.py
+++ b/ddtrace/profiling/collector/_lock.py
@@ -185,6 +185,7 @@ def _release(self, inner_func, *args, **kwargs):
             # _self_acquired_at is only set when the acquire was captured
             # if it's not set, we're not capturing the release
             start = self._self_acquired_at
+            del self._self_acquired_at
 
         try:
             return inner_func(*args, **kwargs)
diff --git a/releasenotes/notes/profiling-lock-acquired-at-remove-c8b5b96130a46ca8.yaml b/releasenotes/notes/profiling-lock-acquired-at-remove-c8b5b96130a46ca8.yaml
new file mode 100644
index 0000000000..fdb268dcd7
--- /dev/null
+++ b/releasenotes/notes/profiling-lock-acquired-at-remove-c8b5b96130a46ca8.yaml
@@ -0,0 +1,5 @@
+---
+fixes:
+  - |
+    profiling: resolves an issue where lock release would have been captured
+    with a wrong acquire timestamp