Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

conflict between TopHat2.1.1 and HTSeq 0.9.1 #50

Open
germaximus opened this issue Dec 19, 2017 · 0 comments
Open

conflict between TopHat2.1.1 and HTSeq 0.9.1 #50

germaximus opened this issue Dec 19, 2017 · 0 comments

Comments

@germaximus
Copy link

Bam files generated by TopHat are incompatible with feature counting software such as HTSeq-count. The reason is likely due to the change to samtools. Tophat uses old samtools 0.1.18. Currently I have to convert bam files to sam and then back to bam with samtools 1.6 to make the suitable for HTSeq-count and subsequent differential gene expression analysis.

Example of the error that HTSeq-count gives on bam files from TopHat
Error occured when reading beginning of SAM/BAM file.
'csamtools.AlignedRead' object has no attribute 'reference_start'
[Exception type: AttributeError, raised in _HTSeq.pyx:1357]

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant