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Error locating program: samtools_0.1.18 #49

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Arctictern6 opened this issue Nov 21, 2017 · 2 comments
Open

Error locating program: samtools_0.1.18 #49

Arctictern6 opened this issue Nov 21, 2017 · 2 comments

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@Arctictern6
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Hello,
I'm trying to map the reads for each sample to the reference genome,and here is the code:

tophat -p 8 -G ./genome/database/GCA_000187875.1_V1.0_genomic.gtf -o ./quantity/DP_1_thout --no-novel-juncs ./genome/database/GCA_000187875.1_V1.0_genome raw_sequences/DP_1_1.fq raw_sequences/DP_1_2.fq

and here is an error:
[2017-11-21 21:08:58] Checking for Bowtie
Bowtie version: 2.2.6.0
Error locating program: samtools_0.1.18

I don't kown why?I have installed the samtools(version:1.5),I will appreciate it if you could help me solve the problem.

best
Lijuan

@kloetzl
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kloetzl commented Dec 7, 2017

I just fixed a similar problem in Debian. You might want to try these changes.

@zxp647
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zxp647 commented Mar 2, 2024

原因是无法调用tophat2文件夹里的samtools_0.1.18程序,可以试试创建软连接
1.提高权限
chmod +x /home/user/tophat2/samtools_0.1.18
2.创建软连接
sudo ln -s /home/user/tophat2/samtools_0.1.18 /usr/bin/samtools_0.1.18

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