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--directional-mapping-reverse vs. --rna-strandness on HISAT3N #432

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paulinarosales opened this issue Jun 17, 2024 · 0 comments
Open

--directional-mapping-reverse vs. --rna-strandness on HISAT3N #432

paulinarosales opened this issue Jun 17, 2024 · 0 comments

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@paulinarosales
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Hello,

I would like to ask about the use of --directional-mapping-reverse on HISAT3N, mainly if the spacification of --rna-strandness RF is needed as well, also if those options are equivalent or both need to be added for reverses stranded libraries?

Thanks!
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@paulinarosales