Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Viral new File format? #280

Open
mwylerCH opened this issue Jun 24, 2024 · 4 comments
Open

Viral new File format? #280

mwylerCH opened this issue Jun 24, 2024 · 4 comments

Comments

@mwylerCH
Copy link

Dear dev team,
it's possible, that the viral file changed again? Centrifuge is not downloading anything for viral.
Here the files:
assembly_summary.txt
assembly_summary_filtered.txt

@mourisl
Copy link
Collaborator

mourisl commented Jun 24, 2024

Thank you for sharing the file. I'll look into this. The filtered file is empty, so the issue should be in the summary.txt file.

@mwylerCH
Copy link
Author

Yes, probably the columns changed.
Many thanks

@mourisl
Copy link
Collaborator

mourisl commented Jul 4, 2024

Sorry for the delayed reply. It seems the awk in centrifuge-download can find some rows in the assembly_summary.txt file. What was your running command? Thank you.

@mwylerCH
Copy link
Author

mwylerCH commented Jul 5, 2024

No problems. I usually use this:

mkdir DB_centrifuge
centrifuge-download -o DB_centrifuge/taxonomy taxonomy
centrifuge-download -P 100 -o DB_centrifuge/library -m -d "archaea,bacteria,viral,vertebrate_mammalian" refseq > DB_centrifuge/seqid2taxid.map

However, today I had to reproduce the error with ...

centrifuge-download -P 100 -o DB_centrifuge/library -m -d "viral" refseq > DB_centrifuge/seqid2taxid.map

... since today also the bacteria part gives me some challenge. The error log is here:
bacteriDownError.txt
Should I open a new issue for that?

Maybe some helpful informations:

  • installation happen with conda with:
git clone https://github.com/DaehwanKimLab/centrifuge
module load Anaconda3/2023.09-0
conda create -n centrifuge
source activate centrifuge
conda install bioconda::centrifuge
  • everything runs on a Rocky 9.3 Blue Onyx cluster with slurm scheduler
  • centrifuge is version 1.0.4
  • all commands are submitted as a wrap with sbatch

Let me knwo if you need further informations.

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants