No CellPhoneDB interacions found in this input.

[ankurnaqib]

Hi. I was running the following command:
cellphonedb method statistical_analysis cellphonedb_meta.txt cellphonedb_countv2.txt --threads 30 --counts-data hgnc_symbol
and I was getting the "No CellPhoneDB interacions found in this input." error.
My data has around 82k cells divided into 6 sample groups, so I followed the suggestions on page Teichlab/cellphonedb#309 and modified my command to :

cellphonedb method statistical_analysis cellphonedb_meta.txt cellphonedb_countv2.txt --threads 30 --counts-data hgnc_symbol --subsampling --subsampling-log False --subsampling-n
um-cells 50 --iterations=10
but I am getting the same error. My data has HGNC gene symbols. Any help would be appreciated. Thank you.

[jsnagai]

Hi @ankurnaqib

82k cells divided into 6 sample groups...Do you mean 6 different phenotypes or 6 cell populations?

Best

[gabumon0]

Is there because of the species? not human, so the gene intersect is zero?

[seoyounglee6445]

i solved this problem. Because my dataset was not normalized dataset. recommended check your dataset.